African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4989

Full Length Research Paper

Detection of mycobacterial skin infections by polymerase chain reaction (PCR) amplification of deoxyribonucleic acid (DNA) isolated from paraffin-embeded tissue

Kasra Behrouznasab1*, Mohammad Reza Razavi2, Hassan Seirafi3, Taher Nejadsattari4, Kumarss Amini5 and Kumarss Amini5      
1Department of microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran. 2Department of parasitology, Pasteur Institute, Tehran, Iran. 3Department of Dermatology, Tehran Medical Sciences University, Tehran, Iran. 4Department of Biology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran. 5Department of Microbiology, Saveh Branch, Islamic Azad University, Saveh, Iran.
Email: [email protected]

  •  Accepted: 18 November 2011
  •  Published: 16 January 2012

Abstract

Atypic mycobacterial granulomatous skin infections are often caused by Mycobacterium marinum, Mycobacterium ulcerans, Mycobacterium fortuitum, Mycobacterium chelonae, and rarely Mycobacterium avium. The lesions appear as papules, nodules in hands, plaque blisters, wart ulcers and markers transmission (sporotrichosis) in the path of lymph glands and lesions; and display a granulomatous accumulation of giant cells. Infection is limited to the skin, and in other cases it could lead to immunosuppression. To determine if mycobacteria were present in granulomatose skin lesion, a total of 58 paraffine tissue blocks were obtained and deoxyribonucleic acid (DNA) isolated the polymerase chain reaction (PCR) that was used to amplify the 16S rRNA gene. PCR amplification demonstrated the presence of Mycobacterium spp. in 18 blocks (31%). Among these 18 blocks, 8 (44%) positive for M. marinum, 33 (17%) for M. ulcerans, 5 isolates (27%) M. fortuitum and M. chelonae, 2 (12%) M. avium. We conclude that mycobacteria ought to be considered in the treatment of skin granulomas in Iran.

Key words: Mycobacterial, granuloma skin infection, polymerase chain reaction (PCR).

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