African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5182

Full Length Research Paper

Anti-biofilm activity of ibuprofen and diclofenac against some biofilm producing Escherichia coli and Klebsiella pneumoniae uropathogens

Rosa Baldiris
  • Rosa Baldiris
  • Grupo de Microbiología Clínica y Ambiental. Universidad de Cartagena. Facultad de Ciencias Exactas y Naturales. Programa de Biología. Campus San Pablo. Cartagena, Colombia.
  • Google Scholar
Victor Teherán
  • Victor Teherán
  • Grupo de Microbiología Clínica y Ambiental. Universidad de Cartagena. Facultad de Ciencias Exactas y Naturales. Programa de Biología. Campus San Pablo. Cartagena, Colombia.
  • Google Scholar
Ricardo Vivas-Reyes
  • Ricardo Vivas-Reyes
  • Grupo de Química Cuántica y Teórica, Universidad de Cartagena, Facultad de Ciencias Exactas y Naturales, Campus San Pablo, Cartagena, Colombia.
  • Google Scholar
Alfredo Montes
  • Alfredo Montes
  • Grupo de Microbiología Clínica y Ambiental. Universidad de Cartagena. Facultad de Ciencias Exactas y Naturales. Programa de Biología. Campus San Pablo. Cartagena, Colombia.
  • Google Scholar
Octavio Arzuza
  • Octavio Arzuza
  • Grupo de Microbiología Clínica y Ambiental. Universidad de Cartagena. Facultad de Ciencias Exactas y Naturales. Programa de Biología. Campus San Pablo. Cartagena, Colombia.
  • Google Scholar


  •  Received: 01 April 2016
  •  Accepted: 20 September 2016
  •  Published: 28 October 2016

Abstract

Ibuprofen (IBU) and Diclofenac (DIC) are non-steroidal anti-inflammatory drug which have shown to have broad antimicrobial activity and limit biofilm formation. Aim of this study was to evaluate the effects of IBU and DIC against biofilm formation strong strains Escherichia coli and Klebsiella pneumoniae associated with urinary tract infections (UTIs). Additionally some virulence factors, antibiotic resistance, ESBL production were estimated to find correlation with the biofilm production and acil homoserine lactone (AHL). Our results suggest that IBU and DIC, could be useful in the treatment of urinary tract infections caused by E. coli and K. pneumoniae. IBU with CMIB 8, 30 and 125 mg/L, caused dramatic reductions in some cases and inhibited virulence factors which changes in the morphotypes expressed on congo red agar. DIC with CMIB 30 and 50 mg/L caused reductions in biofilm formation 50.1%. In this study, were evidenced the relationship between virulence factors, production of AHL and multi drugs resistance with biofilm production.

 

Key words: Diclofenac, ibuprofen, biofilm, acil homoserine lactone (AHL), Escherichia coli, Klebsiella pneumoniae.