African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4935

Full Length Research Paper

Antimicrobial and anti-inflammatory potential of polysaccharide from Pleurotus pulmonarius LAU 09

Adebayo E. A.1*, Oloke J. K.1, Majolagbe O. N.1, Ajani R. A.2 and Bora T. C.3  
  1Department of Pure and Applied Biology, Ladoke Akintola University of Technology, P. M. B. 4000, Ogbomoso, Nigeria. 2Biochemistry Department, Ladoke Akintola University of Technology, P. M. B. 4000, Ogbomoso, Nigeria. 3North East Institute of Science and Technology, 785006 Jorhat, India.
Email: [email protected]

  •  Accepted: 20 March 2012
  •  Published: 09 April 2012

Abstract

The Pleurotus pulmonarius LAU 09 was characterized to the species level and registered at the GenBank database (accession number: JF736658). P. pulmonarius LAU 09 formed a phylogenetic cluster with P. pulmonarius complex with bootstrap value of 77%, which confirmed the close evolutionary lineage with previous characterized P. pulmonariuscomplex. The polysaccharide obtained from P. pulmonarius was structurally characterized and tested for antimicrobial and anti-inflammatory activities. The polysaccharide was active against all tested pathogens except Pseudomonas aeruginosawith percentage activity of 85.75%. The highest zone of inhibition was obtained againstStaphylococcus aureus (30 mm), while the lowest zone size obtained againstEscherichia coli (7 mm). The polysaccharide revealed the potent anti-inflammatory effects against carrageenan and formalin –induced paw edema in rats with higher inhibitory percentage in rats writhing; 83.33% and 92.37% inhibition were obtained in carrageenan and formalin-induced paw edema in rats treated with polysaccharide compared with 33.33 and 84.73% in rats treated with standard (dichlofanac) respectively. The percentage sperm motility were higher in both carrageenan and formalin- induced paw edema in rats treated with polysaccharide (76.25 and 67.95%) while its low in dichlofenac treated rats (47.35 and 63.20%) respectively. The sperm counts were increased considerably in the rats treated with polysaccharide (carrageenan: 44.75 × 106, formalin: 44.80 × 106) compared with the control, 24.70 × 10(carrageenan) and 42.00 × 10(formalin). The significant (p < 0.05) increased obtained in both carrageenan and formalin-induced paw edema in rats treated with polysaccharide in some blood parameters in comparison with dichlofenac treated rats was an evidence of anti-inflammatory potential of the polysaccharide.

 

Key words: Exopolysaccharide, anti-inflammatory, antimicrobial, carrageenan, formalin.

 

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