African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5188

Full Length Research Paper

Comparative assessment of antibacterial activity of uvaria chamae parts

B. B. Oluremi, M. O. Osungunna and O. O. Omafuma
Department of Pharmaceutical Microbiology, Olabisi Onabanjo University, Sagamu, Ogun State, Nigeria.
Email: [email protected]

  •  Published: 04 July 2010

Abstract

 

Methanolic extracts of the root, stem and leaf of Uvaria chamae were evaluated for their antibacterial activity against Methicillin-Resistant Staphylococcus aureus(MRSA), Staphylococcus aureus, Escherichia coliKlebsiella spp, Proteus spp, and typed strains E. coli ATCC 25922, S. aureus ATCC 25923 and Pseudomonas aeruginosa ATCC27853 using standard agar diffusion method at 50, 100, 150, 200 and 250 mg/ml. Preliminary phytochemical screening revealed the presence of alkaloids, tannins, flavonoids, cardiac glycosides and cyanogenic glycosides. In general, the stem bark extract inhibited the growth of all the tested organisms with leaf extract showing the least antibacterial activity. The MIC of methanolic stem extract on E. coli, MRSA, Klebsiella spp, Proteus spp, E. coli ATCC 25922, S. aureusATCC 25923 and P. aeruginosa ATCC 27853 were 5.62, 12.59, 200, 35.48, 28.18, 5.62 and 19.95 mg/ml respectively while that of root extract with the exclusion ofKlebsiella spp were 31.62, 10.0, 3.55 2.82, 12.59 and 39.81 mg/ml respectively. Methanolic leaf extract was found to have MIC 200, 19.95, 250, 250, 250 and 200 mg/ml respectively with no MIC value against P. aeruginosa

 

Key words: Methicillin-resistant, phytochemical screening, alkaloids, cyanogenic, flavonoids.