African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5173

Full Length Research Paper

Structural characterization and antioxidant activity of purified polysaccharide from cultured Cordyceps militaris

Fengyao Wu1, Hui Yan1, Xiaoning Ma1, Junqing Jia1,2, Guozheng Zhang1,2, Xijie Guo1,2 and Zhongzheng Gui1,2*
1Jiangsu University of Science and Technology, Zhenjiang 212018, China. 2Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
Email: [email protected]

  •  Accepted: 15 July 2011
  •  Published: 16 September 2011

Abstract

The polysaccharide fraction, CM-hs-CPS2, was isolated from fruiting bodies of culturedCordyceps militaris grown on solid rice medium by hot water extraction and ethanol precipitation, and purified by ion exchange column (DEAE-cellulose-52) and gel filtration column (Sephadex G-100) chromatography. Its structural characterizations were analyzed by gas chromatograph (GC) and fourier transform infrared spectroscopy (FT-IR). Results indicated that CM-hs-CPS2 was a kind of sulfating acidic polysaccharide containing acetamido group. The monosaccharide composition analysis showed that CM-hs-CPS2 was composed mainly of mannose, glucose and galactose in a molar ration of 1.35: 8.34: 1.00, and linked by α-glycosidic linkage. The studies on antioxidant activities of CM-hs-CPS2 were evaluated by various methods in vitro. Results showed that DPPH· scavenging activity, reducing power and ferrous ion chelating activity of CM-hs-CPS2 (8 mg/ml) were 89%, 1.188 and 85%, respectively.

 

Key words: Cordyceps militaris, polysaccharide, structure, bioactivity.