African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5024

Full Length Research Paper

Genetic diversity analysis of rep-PCR genomic fingerprinting of Lysobacter spp.

FU Li-Na
  • FU Li-Na
  • Key Laboratory of Agriculture Biodiversity for Plant Disease Management under the Ministry of Education, Yunnan Agricultural University, Kunming 650201, P. R. China.
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ZHANG Hai-Yan
  • ZHANG Hai-Yan
  • Key Laboratory of Agriculture Biodiversity for Plant Disease Management under the Ministry of Education, Yunnan Agricultural University, Kunming 650201, P. R. China.
  • Google Scholar
ZHANG Xiao-Fang
  • ZHANG Xiao-Fang
  • Key Laboratory of Agriculture Biodiversity for Plant Disease Management under the Ministry of Education, Yunnan Agricultural University, Kunming 650201, P. R. China.
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WEI Lan-Fang
  • WEI Lan-Fang
  • Key Laboratory of Agriculture Biodiversity for Plant Disease Management under the Ministry of Education, Yunnan Agricultural University, Kunming 650201, P. R. China.
  • Google Scholar
ZHANG Jin-Hao
  • ZHANG Jin-Hao
  • Key Laboratory of Agriculture Biodiversity for Plant Disease Management under the Ministry of Education, Yunnan Agricultural University, Kunming 650201, P. R. China.
  • Google Scholar
JI Guang-Hai
  • JI Guang-Hai
  • Key Laboratory of Agriculture Biodiversity for Plant Disease Management under the Ministry of Education, Yunnan Agricultural University, Kunming 650201, P. R. China.
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  •  Received: 07 July 2016
  •  Accepted: 24 August 2016
  •  Published: 14 September 2016

Abstract

Lysobacter. spp. are considered as important biocontrol bacteria, due to their antagonistic activity against many pathogenic fungi, bacteria and nematodes. Rep-PCR was used to analyze the genetic diversity of 12 Lysobacter strains. These strains included Lysobacter antibioticus (HY, 13-1, 13-6, 6-B-1, 13-B-1, 6-T'-4, LJ6-3, LJ6-4 and LR9-3), Lysobacter enzymogenes (DH3, 1-T-1-4) and Lysobacter capsici (LG18) isolate from different regions in Yunnan province of China. Rep-PCR was performed using DNA amplification with primers based on short bacterial repetitive elements (ERIC, BOX, IS1113 and J3). The genetic diversity was analyzed through rep-PCR, molecular fingerprint clustering analysis and UPGMA to construct phylogenetic tree. The results show that when the genetic distance was 0.59, IS1113-PCR could cluster the Lysobacter strains as 3 species: L. antibioticus, L. enzymogenes and L. capsici. The 3 species had obvious differences between each other and the rep-PCR technique could be used to detect genetic variation between different Lysobacter strains, identification and strain classification. 

 

Key words: Lysobacter, Rep-PCR, genetic diversity.