African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5145

Full Length Research Paper

In vitro antimicrobial activity of maggot excretions/secretions of Sarcophaga (Liopygia) argyrostoma (Robineau-Desvoidy)

Ghada M. El-Bassiony
  • Ghada M. El-Bassiony
  • Department of Entomology, Faculty of Science, Cairo University, Giza, 12613, Egypt.
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John G. Stoffolano, Jr.
  • John G. Stoffolano, Jr.
  • Stockbridge School of Agriculture, College of Natural Sciences, 120 Stockbridge Rd., Fernald Hall, 204A, University of Massachusetts, Amherst, MA 01003, USA.
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  •  Received: 10 May 2016
  •  Accepted: 26 May 2016
  •  Published: 21 July 2016

Abstract

The excessive usage of conventional antibiotics leads to the emergence of multidrug-resistant bacterial strains which threaten public health and stimulates searching for new sources of bio-therapeutic drugs. The aim of this study was to investigate the antimicrobial activity of maggot excretions/secretions from larvae of Sarcophaga argyrostoma, a common species of the family Sarcophagidae in Egypt. The excretions/secretions (ES) produced by third instar larvae were sterile filtered and tested against selected pathogenic strains of Gram positive (Gram+ve) bacteria, Staphylococcus aureus and Bacillus subtilis; Gram negative (Gram-ve) bacteria, Escherichia coli and Pseudomonous aeruginosa; and the filamentous fungus Aspergillus flavus. The ES product produced by third instar maggots proved to be more effective against Gram-ve bacteria. Larval ES, at 0.125 mg/ml concentration, were significantly potent towards P. aeruginosa, E. coli and S. aureus in a descending sequence. The minimum inhibitory concentrations of S. argyrostoma ES were 0.125 mg/ml for P. aeruginosa and E. coli, using the turbidimetric assay method. Twice and four times this concentration were required to inhibit growth of S. aureus (0.25 mg/ml) and B. subtilis (0.5 mg/ml), respectively. The antibacterial properties of S. argyrostoma ES were not affected by heating or freeze-thaw cycles when tested against E. coli.

Key words: Sarcophaga argyrostoma, antimicrobial activity, larval excretions, larval secretions, minimum inhibitory concentration.