African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5238

Full Length Research Paper

Study on rapid detection of seven common foodborne pathogens by gene chip

Jiawang Feng*
  • Jiawang Feng*
  • Zhuhai Entry-Exit Inspection and Quarantine Bureau, Zhuhai 519015, P. R. China.
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Xiaoyan Hu
  • Xiaoyan Hu
  • The Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai 519000, P. R. China.
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Xiaojie Huang
  • Xiaojie Huang
  • South China Agriculture University, Guangzhou 510642, P. R. China.
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Xiaoyu Wang
  • Xiaoyu Wang
  • Zhuhai Entry-Exit Inspection and Quarantine Bureau, Zhuhai 519015, P. R. China.
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Shiming Tang
  • Shiming Tang
  • Zhuhai Entry-Exit Inspection and Quarantine Bureau, Zhuhai 519015, P. R. China.
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Xiaoshan Kuang
  • Xiaoshan Kuang
  • Zhuhai Entry-Exit Inspection and Quarantine Bureau, Zhuhai 519015, P. R. China.
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Danlin Li
  • Danlin Li
  • Zhuhai Entry-Exit Inspection and Quarantine Bureau, Zhuhai 519015, P. R. China.
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Xiaowei Cheng
  • Xiaowei Cheng
  • Zhuhai Entry-Exit Inspection and Quarantine Bureau, Zhuhai 519015, P. R. China.
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  •  Received: 24 March 2015
  •  Accepted: 20 July 2015
  •  Published: 07 March 2016

Abstract

To develop a rapid, effective, specific, and sensitive method to detect foodborne pathogens, 13 sets of primers were designed to amplify the conservative and specific genes of rfbE, fliC, invA, hilA, ipaH, femA, nuc, hlyA, prfA, tuf, speB, tlh and tdh, respectively. Establishment of foodborne pathogens detection chips was conducted by spotting the target genes on the chips by Nano-PlotterTM NP 1.2 printing system. The DNA of 7 standard pathogenic strains and 147 strains extracts from food samples was amplified and labeled for hybridization. The results demonstrated that enterhemorrhagic Escherichia coli O157:H7, Salmonella enteritidis, Shigella flexner, Staphylococcus aureus, Listeria monocytogenes, β-hemolytic streptococcus, and Vibrio parahaemolyticus could correctly be identified by the designed gene chip at an optimal temperature of 58°C and were proved as a potential method with good stability and sensitivity (5 pg/μl of template DNA).

Key words: Gene chip, food-borne pathogen, virulence gene, detection.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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