Proteolysis, lipolysis, as well as, emulsification play important roles for the biological treatment of poultry processing wastewater. Three isolates identified by 16S rRNA gene sequence analysis as belonging to Bacillus subtilis, Bacillus thuringiensis, andLysinibacillus fusiformis, were then selected. The three combined strains provided a 71.2± 0.20% reduction of the chemical oxygen demand (COD) and produced a high biosurfactant 1250 ± 10 mg/L. Moreover, all three bacteria were able to form good biofilm. The combined bacteria strains performed well in terms of COD reduction, protease, and lipase and biosurfactant production at an initial pH of 7.0 and over a wide range (1000 to 5000 mg/L) of organic loads.
Key words: Proteolytic, lipolytic, poultry, wastewater, treatment, biosurfactant.
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