African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5037

Full Length Research Paper

Molecular characterization, biofilm production and sensitivity to the natural extract of Staphylococcus spp. from subclinical mastitis in goats

Rodolfo de Moraes Peixoto
  • Rodolfo de Moraes Peixoto
  • Campus Petrolina Zona Rural, Instituto Federal de Educação, Ciência e Tecnologia do Sertão Pernambucano, PE 647, Km 22, PISNC N - 4, Zona Rural, Cx. Postal 277, CEP 56.302-970, Petrolina, PE, Brazil.
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Renata de Moraes Peixoto Araújo
  • Renata de Moraes Peixoto Araújo
  • Campus de Patos, Universidade Federal de Campina Grande (UFCG), Avenida Universitária, s/n - Santa Cecilia, CEP 58708-110, Patos, PB, Brazil.
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Werônica de Souza Rocha
  • Werônica de Souza Rocha
  • Campus Ciências Agrárias, Universidade Federal do Vale do São Francisco (UNIVASF), Rodov. BR 407 Km 12, Lote 543, Projeto de Irrigação Senador Nilo Coelho s/n, CEP 56300-000, Petrolina, PE, Brazil.
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Luciana Jatobá e Silva Peixoto
  • Luciana Jatobá e Silva Peixoto
  • Campus Petrolina Zona Rural, Instituto Federal de Educação, Ciência e Tecnologia do Sertão Pernambucano, PE 647, Km 22, PISNC N - 4, Zona Rural, Cx. Postal 277, CEP 56.302-970, Petrolina, PE, Brazil.
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Gisele Veneroni Gouveia
  • Gisele Veneroni Gouveia
  • Campus Ciências Agrárias, Universidade Federal do Vale do São Francisco (UNIVASF), Rodov. BR 407 Km 12, Lote 543, Projeto de Irrigação Senador Nilo Coelho s/n, CEP 56300-000, Petrolina, PE, Brazil.
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Jackson Roberto Guedes da Silva Almeida
  • Jackson Roberto Guedes da Silva Almeida
  • Campus Sede, Universidade Federal do Vale do São Francisco (UNIVASF), Av. José de Sá Maniçoba, s/n, Centro, CEP, 56304-917, Petrolina, PE, , Brazil.
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Helinando Pequeno de Oliveira
  • Helinando Pequeno de Oliveira
  • Campus Juazeiro, Universidade Federal do Vale do São Francisco (UNIVASF), Avenida Antonio Carlos Magalhães, 510 - Santo Antônio, CEP 48902-300, Juazeiro, BA, Brazil.
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Mateus Matiuzzi da Costa
  • Mateus Matiuzzi da Costa
  • Campus Ciências Agrárias, Universidade Federal do Vale do São Francisco (UNIVASF), Rodov. BR 407 Km 12, Lote 543, Projeto de Irrigação Senador Nilo Coelho s/n, CEP 56300-000, Petrolina, PE, Brazil.
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  •  Received: 28 March 2020
  •  Accepted: 13 May 2020
  •  Published: 31 July 2020

Abstract

Mastitis is one of the most important diseases in dairy milk systems. Molecular studies of the principal agents that are involved, as well as tests of bacterial sensitivity to plant extracts, are currently the most common forms of research related to this disease. The aim of the present study was to molecularly characterize isolates from cases of subclinical mastitis and to investigate the production of biofilm associated with extracts from Hymenaea martiana. The phytochemical screening of the crude ethanolic extract (CEE) confirmed the presence of phenolic substances, flavonoids, steroids and terpenoids. The antimicrobial susceptibility profile of the isolates that were assessed exhibited variations, with a notably high sensitivity to gentamicin. After the addition of the extract, 77.3, 81.8 and 86.3% of the isolates exhibited a reduction in biofilm production in the ethyl acetate, chloroform and hexane fractions, respectively. An extensive production of biofilm can be observed in the isolates that were not in contact with the natural extract. However, when in contact with the plant extract, there was a reduction in biofilm production, thereby demonstrating the therapeutic potential of the natural extract of H. martiana in mastitis caused by Staphylococcus spp.

 

Key words: Mastitis, pathogens, resistance genes, sensitivity tests.