African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4891

Full Length Research Paper

Development of an enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) to detect and genotype enterotoxigenic Escherichia coli of calf origin

  Xiu-Yan LANG, Yang-Long ZHANG, Hui-Ting JIANG, Jiao LIU and Hong-Bo NI*
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, P.R. China.
Email: [email protected]

  •  Accepted: 03 June 2013
  •  Published: 02 August 2013

Abstract

 

We develop an enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) DNA fingerprinting technique for genotyping enterotoxigenic Escherichia coli(ETEC) in calves. Using the ERIC-PCR, we detected 66 ETEC strains in calves isolated from 11 farms. The ETEC strains could be grouped into 17 types, with type D predominant. The ERIC-PCR system is an efficient method for identification, typing and tracking of ETEC. Serotyping using a specific O antigen revealed 15 serotypes, with serotype O111 most prevalent (21.2%). We were unable to serotype 21.5% of the ETEC strains we had previously identified by ERIC-PCR. Our results indicate that some ETEC strains had identical genotypes, however their serotype might differ and vice versa. The ERIC-PCR method is rapid, sensitive, repeatable and reliable, and we believe it should be applied for the genotyping of ETEC in calves.

 

Key Words: Calf, enterotoxigenic Escherichia coli, ERIC-PCR, genotyping, serotyping.

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