African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Pathogenicity of recent field isolate of Avian Adenovirus Serotype-IV of hydropericardium syndrome (Angara disease)

Shahzad Akbar Khan1*, Tarique hussain2, Om Parkash3 and Sarfaraz Ali Fazlani4      
1Department of Pre-Clinical Studies, Faculty of Veterinary and Animal Sciences, Rawalakot, University of Azad Jammu and Kashmir, Pakistan. 2National Institute of Agriculture and Biology, Faisalabad, Pakistan. 3Department of Animal Husbandry, Government of Sindh, Pakistan. 4Faculty of Veterinary Sciences, Lasbella university of Agriculture, water and Marine Sciences, Uthal, Pakistan.
Email: [email protected]

  •  Accepted: 27 January 2012
  •  Published: 30 May 2012


The pathogenicity of recent field isolate of Avian Adenovirus Serotype IV (AAS-IV) was determined during the present study. During the present investigations, Hydropericardium Hepatitis Syndrome (HHS) showed reduced pathogenicity as compared to the isolates studied on the inception of the syndrome in 1987-1988. Pathogenicity of the virus was determined through LD50 and challenge protection test. The LD50 titre of the 10% viral suspension prepared from liver filtrate was found to be 10-2.5 per ml in 26 days old broiler chicks. None of the chick showed mortality however, Hydropericardium syndrome of Angara disease was observed on postmortem of the challenged chicks and observations grossly observed in the form of lesions on liver, hydronephrosis and water around heart (yellow straw coloured). These findings proved that the injected virus suspension was biologically active.  Formalinized vaccines for HHS including Aqua Base Liver Organ (ABLO) vaccine and Oil Base Tissue Culture (OBTC) vaccine are effective for the control of the syndrome. The vaccination against HHS has positive effect on the immune response of the chicks against HHS. Due to extensive vaccination in the field there is a reduction in the virulence of the virus.


Key words: Pathogenicity, adenovirus, Angara disease, LD50.