African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5173

Full Length Research Paper

β-Fructofuranosidase production by Aspergillus versicolor isolated from Atlantic forest and grown on apple pomace

Taiomara Butkze Dapper
  • Taiomara Butkze Dapper
  • Centro de Ciências Biológicas e da Saúde – Universidade Estadual do Oeste do Paraná - Rua: Universitária 2069, CEP:85814-110, Cascavel, Paraná, Brazil.
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Vanessa Cristina Arfelli
  • Vanessa Cristina Arfelli
  • Centro de Ciências Biológicas e da Saúde – Universidade Estadual do Oeste do Paraná - Rua: Universitária 2069, CEP:85814-110, Cascavel, Paraná, Brazil.
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Caroline Henn
  • Caroline Henn
  • Divisão de Reservatório – MARR.CD, Itaipu. Av. Tancredo Neves 6731, CEP: 85866-900, Foz do Iguaçu, Paraná, Brazil.
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Márcia Regina Simões
  • Márcia Regina Simões
  • Centro de Engenharias e Ciências Exatas – UNIOESTE – Rua da Faculdade, 645, CEP: 85903-000, Toledo, Paraná, Brazil.
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Marise Fonseca dos Santos
  • Marise Fonseca dos Santos
  • Departamento de Biociências, Setor Palotina, Universidade Federal do Paraná – Rua Pioneiro, 2153, CEP: 85950-000, Palotina, Paraná, Brazil.
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Carla Lieko Della Torre
  • Carla Lieko Della Torre
  • Centro de Ciências Médicas e Farmacêuticas – Universidade Estadual do Oeste do Paraná - Rua Universitária, 2069, CEP: 85814-110, Cascavel, Paraná, Brazil.
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Jose Luis da Conceição Silva
  • Jose Luis da Conceição Silva
  • Centro de Ciências Médicas e Farmacêuticas – Universidade Estadual do Oeste do Paraná - Rua Universitária, 2069, CEP: 85814-110, Cascavel, Paraná, Brazil.
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Rita de Cássia Garcia Simão
  • Rita de Cássia Garcia Simão
  • Centro de Ciências Médicas e Farmacêuticas – Universidade Estadual do Oeste do Paraná - Rua Universitária, 2069, CEP: 85814-110, Cascavel, Paraná, Brazil.
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Marina Kimiko Kadowaki*
  • Marina Kimiko Kadowaki*
  • Centro de Ciências Médicas e Farmacêuticas – Universidade Estadual do Oeste do Paraná - Rua Universitária, 2069, CEP: 85814-110, Cascavel, Paraná, Brazil.
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  •  Received: 31 March 2016
  •  Accepted: 01 June 2016
  •  Published: 07 July 2016

Abstract

This study explores the production and characterization of an extracellular β-fructofuranosidase (FFase-I) by Aspergillus versicolor newly isolated from Atlantic Forest-Brazil. The β-fructofuranosidase production by fungus, after the optimization process using central composite design and response surface methodology, showed that 3% (w/v) apple pomace, an initial pH 7.5, and 12 days of cultivation provided the best conditions. The β-fructofuranosidase (FFase-I) was purified from the crude extract by 75% (NH4)2SO4 precipitation followed by DEAE-Sephadex, and the molecular mass of the FFase-I was estimated to be 75 kDa by SDS-PAGE. Furthermore, the enzyme exhibited unusual tolerance to Cu2+, sodium dodecyl sulfate (SDS), Tween 80, ethanol and acetone. The purified enzyme had an optimum pH of 6.0 and was stable over an acidic pH range of 3.0–6.0. The optimum temperature of the FFase-I was 55°C but was stable at 60°C for 7 h. Thus, the β-fructofuranosidase A. versicolor which has thermal stability and activity under acidic conditions would have potential application in sugar cane molasses hydrolysis for subsequent ethanol production.

Key words: Aspergillus, factorial design, fruit wastes, invertase.