African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5145

Full Length Research Paper

Fungi extract in the inhibition of the egg hatching and larval development of the Haemonchus contortus

Thallyta Maria Vieira
  • Thallyta Maria Vieira
  • State University of Montes Claros, Brazil. 2Center for Biological Sciences and Health, Campus Universitário Professor Darcy Ribeiro - Vila Mauricéia - Montes Claros - Minas Gerais, Brasil.
  • Google Scholar
Viviane de Oliveira Vasconcelos
  • Viviane de Oliveira Vasconcelos
  • State University of Montes Claros, Brazil. 2Center for Biological Sciences and Health, Campus Universitário Professor Darcy Ribeiro - Vila Mauricéia - Montes Claros - Minas Gerais, Brasil.
  • Google Scholar
Leydiana Duarte Fonseca
  • Leydiana Duarte Fonseca
  • Federal of São Paulo, Brazil.
  • Google Scholar
Renata Cristina de Souza Cândido
  • Renata Cristina de Souza Cândido
  • Federal University of Minas Gerais, Brazil.
  • Google Scholar
Eduardo Robson Duarte
  • Eduardo Robson Duarte
  • Federal University of Minas Gerais, Brazil.
  • Google Scholar


  •  Received: 24 March 2016
  •  Accepted: 16 June 2016
  •  Published: 21 July 2016

Abstract

In vitro efficacy of aqueous and ethanolic extracts of Paecilomyces lilacinus and Trichoderma longibrachiatum was assessed in the egg hatching inhibition (EHI) and larval development inhibition (LDI) of Haemonchus contortus. For the EHI assessment, after 48 h of incubation with different concentrations of aqueous and ethanolic extracts, it was quantified blastomeres, embryonated eggs, and larvae of first stage. For the LDI assessment, a quantitative coculture adapted method was used. After seven days of feces incubation with different concentrations of aqueous extract of fungi, the infective larvae were collected and quantified, obtaining the number of developed larvae per feces gram. The aqueous extract of P. lilacinus at 1.96 mg mL-1and the ethanolic extract of T. longibrachiatum at 1.90 mg mL-1 completely inhibited the egg hatching. In the LDI, the aqueous extract of T. longibrachiatum at 1.90 mg mL-1 showed efficacy of 92.88%. These fungi extracts showed potential to inhibition of the H. contortus cycle. 

Key words: Paecilomyces lilacinus, Trichoderma longibrachiatum, alternative control, sheep breeding, gastrointestinal nematodes.