African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5056

Full Length Research Paper

Molecular characterization of fowl adenoviruses associated with hydro-pericardium syndrome in broilers

Shah, M. S.1,2* ,Ashraf, A.3, Khan, M. I.1*, Rahman, M.2, Habib, M.4, Babapoor, S.1, Ghaffar, A.2, Malik, I. R.2, Khannum, S. A.4 and  Qureshi, J. A.2,5
  1Department of Pathobiology and Veterinary Sciences, University of Connecticut, Storrs, USA. 2Health Biotechnology Division, National Institute of Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan. 3Department of Bioinformatics and Biotechnology, G.C University, Faisalabad, Pakistan. 4Animal Sciences Division, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, Pakistan. 5Department of Clinical Biochemistry, College of Medicine, King Khalid University, ABHA, Kingdom of Saudi-Arabia.
Email: [email protected], [email protected]

  •  Accepted: 16 November 2011
  •  Published: 16 December 2011

Abstract

 

Hydropericardium syndrome (HPS) is a viral disease of poultry, which is common in hot and humid season during summer in Pakistan. Fowl adenovirus serotype 4 (FAdV-4) classified into the species Fowl adenovirus C and the genus Aviadenoviruswhich were identified as the causative agent of the disease during field outbreaks.  The virus was obtained from suspected broiler birds showing typical signs and postmortem lesions that is ascites, hydropericardium, multifocal hepatic necrosis and hepatitis. The virions were partially purified from the livers of infected birds, by sucrose density gradient ultracentrifugation. A variable part of the hexon gene (L1 and part of P1, 730 bp) was amplified by polymerase chain reaction (PCR) and its nucleotide sequence was determined. During nucleotide sequence analysis, the viruses were identified as FAdV- 4. The presence of FAdV-4 was also supported by scanning electron microscopy. Phylogenetic analysis of the partial hexon gene of the viruses determined their close relation with previously sequenced FAdV-4 isolates from India.

 

Key words: Fowl adenovirus serotype 4 (FAdV-4), hydro-pericardium syndrome, hexon, electron microscopy, phylogenetic analysis, taxonomy.