African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5093

Full Length Research Paper

Assessment of the prevalence of extended-spectrum β-lactamase producing Gram-negative bacilli at the Charles De Gaulle Paediatric University Hospital (CDG-PUH), Ouagadougou, Burkina Faso

Mahamoudou Sanou
  • Mahamoudou Sanou
  • Training and Research Unit in Health Sciences (UFR-SDS), University of Ouagadougou, Ouagadougou, Burkina Faso.
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Absetou Ky/Ba
  • Absetou Ky/Ba
  • Training and Research Unit in Health Sciences (UFR-SDS), University of Ouagadougou, Ouagadougou, Burkina Faso.
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Pricille Coulibali
  • Pricille Coulibali
  • Training and Research Unit in Health Sciences (UFR-SDS), University of Ouagadougou, Ouagadougou, Burkina Faso.
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Marius Nagalo
  • Marius Nagalo
  • Pietro Annigoni biomolecular Research Centre (CERBA)/LABIOGENE, University of Ouagadougou, Burkina Faso.
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Abdoul Salam Ouedraogo
  • Abdoul Salam Ouedraogo
  • Souro Sanou University Hospital (CHU-SS), Bobo-Dioulasso, Burkina Faso.
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Mamadou Tamboura
  • Mamadou Tamboura
  • Charles-de-Gaulle Paediatric University Hospital (CHUP-CDG), Ouagadougou, Burkina Faso.
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Dinanibe Kambire
  • Dinanibe Kambire
  • Charles-de-Gaulle Paediatric University Hospital (CHUP-CDG), Ouagadougou, Burkina Faso.
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Cyrille Bisseye
  • Cyrille Bisseye
  • Pietro Annigoni biomolecular Research Centre (CERBA)/LABIOGENE, University of Ouagadougou, Burkina Faso.
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Fidele Bakiono
  • Fidele Bakiono
  • Armed Forces Health Service, Ouagadougou, Burkina Faso.
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Jacques Simpore
  • Jacques Simpore
  • Pietro Annigoni biomolecular Research Centre (CERBA)/LABIOGENE, University of Ouagadougou, Burkina Faso.
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Ramata Ouedraogo
  • Ramata Ouedraogo
  • Training and Research Unit in Health Sciences (UFR-SDS), University of Ouagadougou, Ouagadougou, Burkina Faso.
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  •  Received: 03 December 2017
  •  Accepted: 22 March 2018
  •  Published: 07 April 2018

Abstract

Bacterial resistance to antibiotics is a serious concern in developing countries due to precarious hygiene conditions, inappropriate prescription as well as self-medication and free sale of antibiotics. This study was aimed to assess the prevalence of strains producing extended-spectrum beta- lactamase in the Gram negative bacteria isolated in the laboratory. The study was conducted in the Charles De Gaulle Paediatric University Hospital (Ouagadougou) and the Arnaud de Villeneuve Regional University Hospital (Montpellier). Out of the 889 pathological substances (pus, urine and blood) analysed, 175 germs were isolated among which 110 were Gram negative bacteria (62.8%). 48.2% of the Gram negative bacteria were positive to extended-spectrum beta-lactamase. Among the 110 Gram negative bacteria isolated, 101 were Enterobacteria and 9 other Gram negative bacteria. No extended-spectrum beta-lactamase was found in the other Gram negative bacteria and 52.5% of Enterobacteria were extended-spectrum beta-lactamase positive. As for the extended-spectrum beta-lactamase phenotype, 60.4% were Escherichia coli and 32% were Klebsiella pneumoniae. 50.9% of extended-spectrum beta-lactamase came from pus and 41.5% from urine. In addition, 64.6% of hospitalised patients had an extended-spectrum beta-lactamase phenotype compared to 24.5% for out-patients. The results show the importance of the phenomenon and should help to better take care of this scourge because antibiotics despite everything, always save millions of lives.
 
Key words: Extended-spectrum beta-lactamase (ESBL), enterobacteria, gram-negative bacilli, inpatients, out-patients.