Comparative in vitro antimicrobial effect of Sarcocephalus latifolius (Sm.) E. A. Bruce leaves and roots on foodborne pathogens

Carlos C. Ahoyo; Pauline M. Deguenon; Durand Dah-Nouvlessounon; Haziz Sina; Thierry D. Houehanou; Alain S. Yaoitcha; Lamine Baba-Moussa; Marcel R. B. Houinato

doi:10.5897/AJMR2019.9062

African Journal of
Microbiology Research

Abbreviation: Afr. J. Microbiol. Res.
Language: English
ISSN: 1996-0808
DOI: 10.5897/AJMR
Start Year: 2007
Published Articles: 5249

Full Length Research Paper

Comparative in vitro antimicrobial effect of Sarcocephalus latifolius (Sm.) E. A. Bruce leaves and roots on foodborne pathogens

Carlos C. Ahoyo

Carlos C. Ahoyo
Laboratory of Applied Ecology, Faculty of Agronomic Sciences, University of Abomey-Calavi, 01 P.O. Box 526 Cotonou, Republic of Benin.
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Pauline M. Deguenon

Pauline M. Deguenon
Laboratory of Applied Ecology, Faculty of Agronomic Sciences, University of Abomey-Calavi, 01 P.O. Box 526 Cotonou, Republic of Benin.
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Durand Dah-Nouvlessounon

Durand Dah-Nouvlessounon
Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Sciences and Technology, University of Abomey-Calavi, 05 PO Box 1604, Abomey-Calavi, Republic of Benin.
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Haziz Sina

Haziz Sina
Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Sciences and Technology, University of Abomey-Calavi, 05 PO Box 1604, Abomey-Calavi, Republic of Benin.
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Thierry D. Houehanou

Thierry D. Houehanou
Laboratory of Applied Ecology, Faculty of Agronomic Sciences, University of Abomey-Calavi, 01 P.O. Box 526 Cotonou, Republic of Benin.
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Alain S. Yaoitcha

Alain S. Yaoitcha
Laboratory of Applied Ecology, Faculty of Agronomic Sciences, University of Abomey-Calavi, 01 P.O. Box 526 Cotonou, Republic of Benin.
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Lamine Baba-Moussa

Lamine Baba-Moussa
Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Sciences and Technology, University of Abomey-Calavi, 05 PO Box 1604, Abomey-Calavi, Republic of Benin.
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Marcel R. B. Houinato

Marcel R. B. Houinato
Laboratory of Applied Ecology, Faculty of Agronomic Sciences, University of Abomey-Calavi, 01 P.O. Box 526 Cotonou, Republic of Benin.
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Article Number - BAB871C61303
Vol.13(22), pp. 357-368 , June 2019
https://doi.org/10.5897/AJMR2019.9062

Received: 29 January 2019
Accepted: 29 April 2019
Published: 14 June 2019

Abstract

Sarcocephalus latifolius (Sm.) E. A. Bruce is a plant used traditionally to treat a wide range of infectious diseases, including foodborne ones. This study aimed to compare its roots and leaves extract antibacterial effects in vitro. Thus, these organs were collected, dried and powdered for the extractions and phytochemical screening. Four extracts (water, ethyl-acetate, methanol and ethanol) were tested on ten references strains (RS) and nine non references strains of Staphylococcus isolated from meat strains (MS) using disk method. Minimal inhibitory (MIC) and bactericidal concentrations (MBC) were determined respectively by macrodilution method and by solid culture medium. The results showed that the MS seem more susceptible than RS. The lowest activity was shown by roots hydro-ethanolic extract on Micrococcus luteus, whereas the highest was obtained with leaves ethanolic extract on Staphylococcus cohini. The MIC ranges from 0.312 to 25 mg/ml for the MS and from 6.25 to 12.50 mg/ml for the RS. About MBC, they vary from 12.50 to 50 mg/ml for all susceptible tested microorganisms. S. latifolius extracts have an inhibitory effect on both strains such as Staphylococcus xylosus, Escherichia coli O157, Staphylococcus sciuri, Staphylococcus aureus, M. luteus, Candida albicans, S. cohinii, Proteus mirabilis and bactericidal effect on the three last ones. These results support the traditional use of S. latifolius in infectious diseases control; furthermore, its leaves seem more effective than the roots. These findings may serve as a starting point for the development of a new drug for the control of this kind of diseases and could sustain the species use.