African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5173

Full Length Research Paper

Resistance profile of Staphylococcus strains and detection of the Mec A, Van A and Van B genes in private hospitals in Benin

Prosper Adzavon
  • Prosper Adzavon
  • Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Benin.
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Tamegnon Victorien Dougnon
  • Tamegnon Victorien Dougnon
  • Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Benin.
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Hornel Koudokpon
  • Hornel Koudokpon
  • Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Benin.
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Boris Legba
  • Boris Legba
  • Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Benin.
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Alidehou Jerrold Agbankpe
  • Alidehou Jerrold Agbankpe
  • Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Benin.
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Kafayath Fabiyi
  • Kafayath Fabiyi
  • Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Benin.
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Lamine Baba-Moussa
  • Lamine Baba-Moussa
  • Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Benin.
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  •  Received: 27 April 2021
  •  Accepted: 03 June 2021
  •  Published: 31 May 2022

Abstract

The present study aims to identify the emergence of Methicillin-resistant Staphylococcus aureus, S. aureus resistant to vancomycin and to investigate the presence of Mec A, Van A and Van B genes among Staphylococcus strains isolated from hospital environment. For each type of sample, surface of beds, recyclable material, boxes of rodar, floor and door slats, 53 samples were taken. So, a total of 265 samples were collected by swabbing (except boxes of rodar) in a private clinic in southern Benin. Bacteriological analysis was performed using the conventional method followed by DNA extraction with the Quiagen kit. The resistance genes Mec A, Van A and Van B were sought using specific primers. 215 samples were culture positive with 155 strains (62%) of coagulase negative (CNS) staphylococci and 95 strains (38%) of S. aureus. The majority of strains were resistant to gentamycin and clindamycin. These 155 strains were carried the Mec A gene and 10 strains carried the Van A and Van B gene. The study reveals the presence of resistant Staphylococci carrying the Mec A gene, which could be responsible for nosocomial infections in patients. Hygiene must be improved to limit the spread of these germs and protect patients.

 

Key words: Nosocomial infections, Staphylococcus, resistance antimicrobial Mec A, Van A and Van B gene.