African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5056

Full Length Research Paper

Detection of Clostridium tetani by fluorescent amplification based specific hybridization

  Mohammad Saadatmandzadeh1, Hossein Eshghi 1*, Mohammad Rahimizadeh1, Mojtaba Sankian2 and Saeid Amel Jamehdar3
  1Department of Chemistry, School of Sciences, Ferdowsi University of Mashhad, Mashhad 91775-1436, Iran. 2Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran. 3Microbiology and Virology Research Center, Avicenna Research Institute, Department of bacteriology and Virology, Ghaem University Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Email: [email protected]

  •  Accepted: 20 October 2011
  •  Published: 16 December 2011

Abstract

 

The etiologic agent of tetanus, Clostridium tetani, is an obligate anaerobic and spore-forming bacillus; and remains an important public health problem in developing countries. Although, the diagnosis is based mainly on history and presentation of tetanus symptoms. Some recent reports indicate that a patient may not manifest any symptoms of localized or generalized tetanus. However, one of the new approaches for accurate and rapid detection of microbial pathogens is a polymerase chain reaction (PCR) followed by fluorescence detection. In this report, we have described a fluorescent amplification based specific hybridization (FLASH) based PCR assay for detection of C. tetani. The results of the present study indicated that the FLASH based PCR assay appear to be a suitable and rapid method for identification of cultured isolates of C. tetani.

 

Key words: Clostridium tetani, molecular diagnosis, fluorescent amplification based specific hybridization (FLASH) polymerase chain reaction (PCR), fluorescent probe.