African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5090

Full Length Research Paper

Variability in the genotypes of rotavirus detected in Côte d'Ivoire from 2010-2016

Boni-Cisse Catherine
  • Boni-Cisse Catherine
  • Département de Microbiologie, Université Félix Houphouët Boigny, UFR des Sciences Médicales, Côte d’Ivoire.
  • Google Scholar
Meite syndou
  • Meite syndou
  • Département de Microbiologie, Université Félix Houphouët Boigny, UFR des Sciences Médicales, Côte d’Ivoire.
  • Google Scholar
Britoh Mlan Alice
  • Britoh Mlan Alice
  • Département de Microbiologie, Université Félix Houphouët Boigny, UFR des Sciences Médicales, Côte d’Ivoire.
  • Google Scholar
Zaba Flore
  • Zaba Flore
  • Laboratory of Sentinel Site Surveillance of Paediatric Bacterial Meningitis and Rotavirus Diarrhoea, CHU, Yopougon, Abidjan, Côte d’Ivoire.
  • Google Scholar
N’Guessan Rebecca
  • N’Guessan Rebecca
  • Paediatric Service, CHU, Yopougon, Abidjan, Côte d’Ivoire.
  • Google Scholar
Aka Lepri Nicaise
  • Aka Lepri Nicaise
  • Expanded Program on Immunization, Côte d'Ivoire.
  • Google Scholar
Lartey Belinda
  • Lartey Belinda
  • NMIMR West African Regional Rotavirus Reference Laboratory, Accra, Ghana.
  • Google Scholar


  •  Received: 27 September 2017
  •  Accepted: 13 November 2017
  •  Published: 14 December 2017

Abstract

Group A rotaviruses are the major viral agent of acute gastroenteritis and severe diarrhea in children <5 years old. The World Health Organization (WHO) recommends surveillance of circulating strains before and after introduction of vaccination in countries. However, the diversity of circulating strains in developing countries is a major challenge to the vaccination programs. This study, carried out in furtherance of the sentinel surveillance, aims to identify the different genotypes circulating before the introduction of the Rotavirus vaccine. All children with acute gastroenteritis aged 0 to 5 years, admitted in one of the sentinel surveillance collection sites were included in the study. The study period was from January 2010 to December 2016. Rotavirus was detected in stool specimens by enzyme-linked immunosorbent assay (ELISA). Rotavirus G and P types were determined by real-time polymerase chain reaction (RT-PCR). A total of 1472 stool samples were collected during this period. 31.8% of the stools were rotavirus positive by ELISA test. G1 was predominant with 39.6% followed by G12 (27%). P [8] was 50.4%. The predominant genotype combinations were G1P [8] with 26.1%; G12P [8], 15%; G1P [6], 11.3% and G12P [6], 10.8%. Genotyping of circulating rotavirus strains is important in monitoring strains before and after the introduction of the vaccine. With previous observations, these findings will contribute to baseline data to further monitor the impact of rotavirus immunization in Côte d’Ivoire. 
 
Key words: Rotavirus, Côte d’Ivoire, diarrhea, vaccination, acute gastroenteritis.