African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5092

Full Length Research Paper

Salmonella Heidelberg reduces nitrergic neurons in the myenteric plexus of the duodenum of broilers

Patricia Franco Goncalves Previato do Amaral
  • Patricia Franco Goncalves Previato do Amaral
  • Academics of the Postgraduate Program in Animal Science with Emphasis on Bioactive Products, Universidade Paranaense - UNIPAR, Umuarama, Paraná, Brazil.
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Gabriela Rocha Santos
  • Gabriela Rocha Santos
  • Academic of the Veterinary Medicine, Universidade Paranaense - UNIPAR, Umuarama, Paraná, Brazil.
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Tatiana Kaori Urano
  • Tatiana Kaori Urano
  • Academic of the Veterinary Medicine, Universidade Paranaense - UNIPAR, Umuarama, Paraná, Brazil.
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Karen da Silva Diniz
  • Karen da Silva Diniz
  • Academic of the Pharmacy, Universidade Paranaense - UNIPAR, Paranavaí, Paraná, Brazil.
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Tatiane dos Santos Aparecido Goncalves
  • Tatiane dos Santos Aparecido Goncalves
  • Academics of the Postgraduate Program in Animal Science with Emphasis on Bioactive Products, Universidade Paranaense - UNIPAR, Umuarama, Paraná, Brazil.
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Grazielli de Fatima Serenini
  • Grazielli de Fatima Serenini
  • Academics of the Postgraduate Program in Animal Science with Emphasis on Bioactive Products, Universidade Paranaense - UNIPAR, Umuarama, Paraná, Brazil.
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Luciana Kazue Otutumi
  • Luciana Kazue Otutumi
  • Professor of the Postgraduate Program in Animal Science with Emphasis on Bioactive Products, Universidade Paranaense - UNIPAR, Umuarama, Paraná, Brazil.
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Ricardo de Melo Germano
  • Ricardo de Melo Germano
  • Professor of the Postgraduate Program in Animal Science with Emphasis on Bioactive Products, Universidade Paranaense - UNIPAR, Umuarama, Paraná, Brazil.
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  •  Received: 16 May 2017
  •  Accepted: 14 July 2017
  •  Published: 07 September 2017

Abstract

The present study aimed to characterize the effects of Salmonella Heidelberg challenge, as well as its changes in the inhibitory neuron subpopulation in the myenteric plexus of broilers. At the age of 21 days, after euthanasia of birds, duodenum fragments were collected from 5 male chicken broilers Cobb 500 linage from the challenged group and the control group for the quantification from the subpopulation of myenteric plexus inhibitory neurons by the histochemical method of NADPH-diaphorase. The membrane preparations obtained after neuronal marking were used for quantifying the neurons from micrographs obtained under light microscope, corresponding to 60 microscope fields per animal. Regarding the location of the neurons, they were grouped into those forming ganglia and those isolated between the fibers connecting the neuron groupings. The average number of neurons obtained was 24.38 neurons per mm2 in the control group, and 9.57 neurons per mm2 in the challenged group, with such values presenting statistical variation. This reduction in the number of neurons is perhaps a sign of the deleterious effects of Salmonella Heidelberg on positive NADPH-diaphorase neurons. Such reduction may promote changes in the functional characteristics of the digestive tube, reducing or slowing down the processes of digestion and absorption of nutrients, thereby limiting the animals’ productive performance.

Key words: Gallus gallus, NADPH-diaphorase, inhibitory neurons, enteric nervous system, Salmonella sp.