African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5045

Full Length Research Paper

Preparation, characterization and in vitro antimicrobial activity of compound sustained-release periodontal suppository of ornidazole and pefloxacin mesylate

Rui Liu, Yan Jiang, Yan-hua Duan, Nan Li, Guo-dong Zhang, Xin Nie and Lu-chuan Liu*
Department of Stomatology, Research Institute of Surgery and Daping Hospital, the Third Military Medical University, Chongqing 400042, China.
Email: [email protected]

  •  Accepted: 26 August 2011
  •  Published: 30 December 2011

Abstract

 

Local delivery of sustained-release drugs in periodontal pocket is an effective approach for the treatment of periodontitis. The present study aimed to optimize and characterize the sustained-release periodontal suppository of compound ornidazole and pefloxacin mesylate (O&P) in vitro. Before suppository was prepared, the combined effect of O&P against dominant anaerobe and facultative anaerobe in periodontitis was investigated and then the optimal ratio of each drug was determined. After the compound suppository of O&P was prepared by cold compression, the optimal content of the suppository was determined through evaluating the bacteriostatic effect and then the sustained-release level of optimized suppository was investigated. There was no incompatibility between ornidazole and pefloxacin mesylate. When being applied in combination, they could exert synergistic bacteriostatic effect against ‘porphyromonas gingivalid’ and ‘fusobacterium nucleatum’, and additive effect against ‘prevotella intermedia’ and ‘peptostreptococcus spp’ and inhibit anaerobe growth at a low concentration. The optimal ratio of O&P is 2:3 and the optimal content of suppository 20% in vitro; this suppository could release both drugs in a sustained manner for 12 h. The compound suppository of O&P can serve as one of the potential candidates for the treatment of periodontitis and has favorable sustained-release ability which can meet the requirement of clinical treatment of periodontitis.

 

Key words: Ornidazole, pefloxacin mesylate, periodontitis, sustained-release delivery system.