African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4983

Full Length Research Paper

Detection of multidrug-resistant enterobacteria simultaneously producing extended-spectrum β-lactamases of the PER and GES types isolated at Saint Camille Hospital Center, Ouagadougou, Burkina Faso

Mètuor Dabiré Amana
  • Mètuor Dabiré Amana
  • Laboratory of Biology and Genetics Molecular (LABIOGENE), University Ouaga 1 Pr Joseph KI-ZERBO (UOI-PJKZ), B.P: 03 B.P. 7021 Ouagadougou 03, Burkina Faso.
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Tiemtoré Rahimatou Yasmine Wend-Kuni
  • Tiemtoré Rahimatou Yasmine Wend-Kuni
  • Laboratory of Biology and Genetics Molecular (LABIOGENE), University Ouaga 1 Pr Joseph KI-ZERBO (UOI-PJKZ), B.P: 03 B.P. 7021 Ouagadougou 03, Burkina Faso.
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Bangré Yasmine Aminata
  • Bangré Yasmine Aminata
  • Laboratory of Applied Biochemistry and Chemistry (LABIOCA), UFR-SVT, University Ouaga 1 Pr Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Zohoncon Théodora Mahoukédé
  • Zohoncon Théodora Mahoukédé
  • Laboratory of Biology and Genetics Molecular (LABIOGENE), University Ouaga 1 Pr Joseph KI-ZERBO (UOI-PJKZ), B.P: 03 B.P. 7021 Ouagadougou 03, Burkina Faso.
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Sougué Serge
  • Sougué Serge
  • Laboratory of Biology and Genetics Molecular (LABIOGENE), University Ouaga 1 Pr Joseph KI-ZERBO (UOI-PJKZ), B.P: 03 B.P. 7021 Ouagadougou 03, Burkina Faso.
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Zongo Jacob Koudbi
  • Zongo Jacob Koudbi
  • Laboratory of Biology and Genetics Molecular (LABIOGENE), University Ouaga 1 Pr Joseph KI-ZERBO (UOI-PJKZ), B.P: 03 B.P. 7021 Ouagadougou 03, Burkina Faso.
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Simporé Jacques
  • Simporé Jacques
  • Laboratory of Biology and Genetics Molecular (LABIOGENE), University Ouaga 1 Pr Joseph KI-ZERBO (UOI-PJKZ), B.P: 03 B.P. 7021 Ouagadougou 03, Burkina Faso.
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  •  Received: 16 May 2019
  •  Accepted: 22 July 2019
  •  Published: 31 August 2019

Abstract

Resistance to a wide variety of common antibiotics is observed among clinical strains designated as extended-spectrum β-lactamase (ESBL) producers. These produce enzymatic proteins that effectively inactivate cephalosporins and aztreonam and are a serious global health problem that complicates treatment strategies. Many studies report a high prevalence of ESBL producers among Gram-negative bacilli. The purpose of this work was to identify resistance genes in enterobacterial strains. Gram-negative bacilli resistant to at least one third-generation cephalosporin, aztreonam or showing a synergy image between amoxicillin + clavulanic acid and a third generation cephalosporin were isolated during an antibiogram. Antibiotic resistance was detected for the following antibiotics: Ceftriaxone, Cefotaxime, Ceftazidime and Aztreonam. Classical polymerase chain reaction (PCR) analyzes of Pseudomonas extended resistance (PER) and Guiana extended-spectrum (GES) β-lactamase genes were performed using specific primers in 60 ESBL-producing isolates. Among 250 strains of Gram negative bacilli collected, 60 strains  (24%) showed resistance to antibiotics used. Stool samples are a major source of ESBL producers. The highest prevalence of resistant strains (35%) was observed in Escherichia coli. The GES and PER genes were simultaneously detected at a proportion of 13.33%. This study represents the first detection of PER and GES genes in multidrug-resistant enterobacteria in Burkina Faso.

 

Key words: Broad-spectrum beta-lactamases (ESBLs), Pseudomonas extended resistance (PER) gene, Guiana extended-spectrum (GES) gene, polymerase chain reaction (PCR).