African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5182

Full Length Research Paper

Survival of Salmonella Enteritidis and Escherichia coli in cactus cladodes under domestic marketing conditions in Mexico

Jose Luis Zarate-Castrejon
  • Jose Luis Zarate-Castrejon
  • Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Campo Experimental Bajío. Km. 6.5 Carretera Celaya-San Miguel de Allende, C.P. 38110, Celaya, Guanajuato, México
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Talina Olivia Martínez-Martínez*
  • Talina Olivia Martínez-Martínez*
  • Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Campo Experimental Bajío. Km. 6.5 Carretera Celaya-San Miguel de Allende, C.P. 38110, Celaya, Guanajuato, México
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Juan Gabriel Angeles-Nunez
  • Juan Gabriel Angeles-Nunez
  • Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Campo Experimental Bajío. Km. 6.5 Carretera Celaya-San Miguel de Allende, C.P. 38110, Celaya, Guanajuato, México
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Fanny Guadalupe Concha-Valdez
  • Fanny Guadalupe Concha-Valdez
  • Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán. Mérida, Yucatán Avenida Itzáes # 490 x Calle 59, Colonia Centro C.P. 97000 Mérida, Yucatán, Mexico.
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Jose Ramon Verde-Calvo
  • Jose Ramon Verde-Calvo
  • Universidad Autónoma Metropolitana. Depto. de Biotecnología, Laboratorio de Enología y Alimentos Fermentados. México, D.F. México. Av. San Rafael Atlixco No. 186, Col. Vicentina, Del. Iztapalapa. C.P. 09340. México, D.F. México.
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Martha Elva Ramirez-Guzman
  • Martha Elva Ramirez-Guzman
  • Colegio de Postgraduados, Campus Montecillo, Colegio de Postgraduados, Carretera México-Texcoco, Km. 36.5, Texcoco, México.
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  •  Received: 07 July 2016
  •  Accepted: 01 December 2016
  •  Published: 21 December 2016

Abstract

In Mexico, during domestic marketing of cactus cladodes, called “nopalitos”, there is a tendency not to provide refrigerated storage, and sometimes dealers do not take care of the hygienic conditions of the product during commercialization. Therefore, the objective of this study was to evaluate the survival of Salmonella Enteritidis and Escherichia coli on cladodes of Opuntia ficus-indica Var. Atlixco under conditions associated with domestic marketing in Mexico. Some phenolic compounds present in cladodes, which could influence the survival of these pathogens in the vegetable were analyzed at the same time. For the survival experiment, a 2x2 factorial design was used; treatments included two presentations of cactus cladodes (without spines and with spines) and two storage temperatures. Viable cells were counted during 16 days of storage using specific culture media. Phenolic compounds were determined using HPLC. E. coli did not survive on cactus without spines during 16 days at 4°C, while S. Enteritidis was able to survive until 16 days in all the treatments. The analytic results obtained indicated higher contents of caffeic and protocatechuic acid. The results showed the importance of refrigerated storage of nopalitos during their commercialization to reduce the risk of presence of foodborne illness and provide good practices during marketing.

Key words: Opuntia ficus-indica, human pathogens, handling, storage temperature.