African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5183

Full Length Research Paper

Effect of temperature, pH and substrate composition on production of lipopeptides by Bacillus amyloliquefaciens 629

Fernando Pereira Monteiro
  • Fernando Pereira Monteiro
  • UNIVAG Centro Universitário, Av. Dom Orlando Chaves, no. 2.655, Bairro Cristo Rei - Várzea Grande, Mato Grosso, CEP 78118-900, Brasil.
  • Google Scholar
Flavio Henrique Vasconcelos de Medeiros
  • Flavio Henrique Vasconcelos de Medeiros
  • Universidade Federal de Lavras (UFLA), Campus Universitário, Caixa Postal 3037, CEP 37200-000, Lavras, Minas Gerais, Brazil.
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Marc Ongena
  • Marc Ongena
  • Université de Liège - Gembloux AgroBiotech, Passage des Déportés, 2, 5030, Gembloux, Belgium.
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Laurent Franzil
  • Laurent Franzil
  • Université de Liège - Gembloux AgroBiotech, Passage des Déportés, 2, 5030, Gembloux, Belgium.
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Paulo Estevao de Souza
  • Paulo Estevao de Souza
  • Universidade Federal de Lavras (UFLA), Campus Universitário, Caixa Postal 3037, CEP 37200-000, Lavras, Minas Gerais, Brazil.
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Jorge Teodoro de Souza
  • Jorge Teodoro de Souza
  • Universidade Federal de Lavras (UFLA), Campus Universitário, Caixa Postal 3037, CEP 37200-000, Lavras, Minas Gerais, Brazil.
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  •  Received: 20 July 2016
  •  Accepted: 07 September 2016
  •  Published: 28 September 2016

Abstract

The lipopeptides surfactin, fengycin and iturin produced by Bacillus species have diverse ecological roles, including antimicrobial activity, induced systemic resistance in plants against pathogens and plant colonization by the producing strain. The conditions that govern both lipopeptide production and plant colonization are not fully understood. The present study investigated the role of growth media, temperature and pH on the production of the lipopeptides surfactin, fengycin and iturin by Bacillus amyloliquefaciens 629 and its production in bean plants colonized epiphytically and endophytically by this isolate. Surfactin was produced at higher amounts when isolate 629 was grown at 15 than at 25 and 30ºC, whereas fengycin remained approximately constant across different temperatures. Iturin was detected on bean stem sap, root exudates and in potato dextrose broth, indicating that plant-derived nutrients play an important role in its production by isolate 629. B. amyloliquefaciens 629 colonized plants with more efficacy at 28 than at 20°C. None of the lipopeptides was detected in plants colonized by isolate 629, despite the number of attempts performed with ultra-performance liquid chromatography (UPLC) analysis.

 

Key words: Bioaccumulation, fengycin, iturin, surfactin.