African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5045

Full Length Research Paper

Diversity of nifH gene sequences in the sediments of South China Sea

Lixian Wu1,2#, Yanhua Cui3# and Sanfeng Chen1*
  1State Key Laboratory of Agrobiotechnology and College of Biological Sciences, China Agricultural University, Beijing 100193, China. 2Department of Pathogen Biology, Hainan Medical University, Haikou 571101, China. 3School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China.
Email: [email protected]

  •  Accepted: 21 October 2011
  •  Published: 30 December 2011

Abstract

 

In order to contribute to knowledge about structure of marine diazotrophic communities in the sediments of South China Sea, the molecular diversity of the nifH gene, which encodes the Fe protein of the nitrogenase complex, was assessed by polymerase chain reaction (PCR) amplification using PolF/R primers, followed by cloning and sequencing. Sequences of nifH genes were amplified from environmental deoxyribonucleic acid (DNA) samples collected during three stations including shallow sea (75 m, station L10), shelf (450 m, station L2) and deep sea (1000 m, station L21), and covering an area between 17 to 19°N and 111 to 119°E. Samples from shallow sea contained β-, and δ- proteobacteria; the shelf contained α-, β-, δ- proteobacteria; the deep sea contained α-, δ-, γ-proteobacteria, firmicutes, and green nonsulfur (GNS) bacterium. These results suggested that diazotroph was significant component potentially contributing to nitrogen fixation in South China Sea. 

 

Key wordsnifH, diversity, sediment, the South China Sea.