African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5183

Full Length Research Paper

Bacteria associated with sugarcane in Northeastern Brazil

Michelangelo de Oliveira Silva
  • Michelangelo de Oliveira Silva
  • Federal Institute of Science, Education, and Technology of Alagoas, Campus Piranhas, Sergipe Avenue, 57460-000 Piranhas, Alagoas, Brazil.
  • Google Scholar
Fernando José Freire
  • Fernando José Freire
  • Federal Rural University of Pernambuco, Dom Manoel de Medeiros Avenue, University Campus of Dois Irmãos, 52171-900 Recife, Pernambuco, Brazil.
  • Google Scholar
Júlia Kuklinsky-Sobral
  • Júlia Kuklinsky-Sobral
  • Federal Rural University of Pernambuco, Academic Unit of Garanhuns, Bom Pastor Avenue, 55292-270 Garanhuns, Pernambuco, Brazil.
  • Google Scholar
Emídio Cantídio Almeida de Oliveira
  • Emídio Cantídio Almeida de Oliveira
  • Federal Rural University of Pernambuco, Dom Manoel de Medeiros Avenue, University Campus of Dois Irmãos, 52171-900 Recife, Pernambuco, Brazil.
  • Google Scholar
Maria Betânia Galvão dos Santos Freire
  • Maria Betânia Galvão dos Santos Freire
  • Federal Rural University of Pernambuco, Dom Manoel de Medeiros Avenue, University Campus of Dois Irmãos, 52171-900 Recife, Pernambuco, Brazil.
  • Google Scholar
Valéria Xavier de Oliveira Apolinário
  • Valéria Xavier de Oliveira Apolinário
  • Federal Rural University of Pernambuco, Dom Manoel de Medeiros Avenue, University Campus of Dois Irmãos, 52171-900 Recife, Pernambuco, Brazil.
  • Google Scholar


  •  Received: 29 July 2016
  •  Accepted: 27 September 2016
  •  Published: 07 October 2016

Abstract

Sugarcane has a high demand in nitrogen, increasing costs and causing damages to the environment. It is necessary to find alternatives to reduce nitrogen fertilizers use. Diazotrophic bacteria have capacity to promote growth in grass with potential to fix N2 and solubilize inorganic phosphate. This study aimed to evaluate bacterial community associated with different sugarcane varieties in Northeastern Brazil, select bacteria with plant growth-promoting characteristics, and identify endophytic and epiphytic bacterial lineages in sugarcane. Endophytic bacteria of leaves and roots and epiphytic bacteria of rhizoplane were isolated from three sugarcane commercial varieties and selected for their capacity to fix N2 and solubilize inorganic phosphate. Bacterial strains from different morphological groups were isolated and a sample of 27 strains with potential for the simultaneous development of these characteristics were selected and identified. The bacterial community that interacted with sugarcane was more associated with rhizoplane and roots regions than with leaves, and had a high potential to fix N2 and solubilize inorganic phosphate. Bacterial lineages were mainly from genera Pantoea sp. and Burkholderia sp., but there were also genera Enterobacter sp., Klebsiella sp. and Pseudomonas sp. and two lineages at the species level: Pantoea stewartii and Burkholderia cenocepacia.

Key words: Plant/bacteria interaction, plant growth-promoting bacteria, N2 fixing bacteria, bacteria identification.