African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4881

Full Length Research Paper

Biofertilising, plant-stimulating and biocontrol potentials of maize plant growth promoting rhizobacteria isolated in central and northern Benin

Nadège Adoukè Agbodjato
  • Nadège Adoukè Agbodjato
  • Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 05 BP 1604 Cotonou, Bénin.
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Olaréwadjou Amogou
  • Olaréwadjou Amogou
  • Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 05 BP 1604 Cotonou, Bénin.
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Pacôme Agossou Noumavo
  • Pacôme Agossou Noumavo
  • Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 05 BP 1604 Cotonou, Bénin.
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Gustave Dagbénonbakin
  • Gustave Dagbénonbakin
  • Centre de Recherches Agricoles d’Agonkanmey, Institut National des Recherches Agricoles du Bénin, Bénin.
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Hafiz Adio Salami
  • Hafiz Adio Salami
  • Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 05 BP 1604 Cotonou, Bénin.
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Rachidath Karimou
  • Rachidath Karimou
  • Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 05 BP 1604 Cotonou, Bénin.
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Abdel-Madjid Alladé
  • Abdel-Madjid Alladé
  • Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 05 BP 1604 Cotonou, Bénin.
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Oyedele Adedayo
  • Oyedele Adedayo
  • Institute of Agricultural Research and Training, Moor plantation, Ibadan, Nigeria.
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Farid Baba-Moussa
  • Farid Baba-Moussa
  • Laboratoire de Microbiologie et des Technologies Alimentaires, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 04 BP 1107 Cotonou, Bénin.
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Adolphe Adjanohoun
  • Adolphe Adjanohoun
  • Centre de Recherches Agricoles Sud, Institut National des Recherches Agricoles du Bénin, BP 03 Attogon, Bénin.
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Lamine Saïd Baba-Moussa
  • Lamine Saïd Baba-Moussa
  • Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 05 BP 1604 Cotonou, Bénin.
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  •  Received: 12 June 2018
  •  Accepted: 19 July 2018
  •  Published: 28 July 2018

Abstract

Plants constantly interact with a multitude of microorganisms that they select among other things through their roots. Some bacteria, known as plant growth promoting rhizobacteria (PGPR), are able to stimulate growth and control plant diseases, thanks to the expression of a wide range of beneficial properties to the plant. The aim of this work was to search for biofertilizing, plant-stimulating and biocontrol potentials in PGPR in central and northern Benin. To achieve this goal, the metabolic properties, especially phosphate solubilization, the production of indole acetic acid, hydrogen cyanide, ammonia, exopolysaccharides, certain enzymes and antifungal activity were investigated on nine rhizobacteria strains: Bacillus polymysa, Bacillus anthracis, Bacillus circulans, Bacillus thuringiensis, Bacillus panthothenicus, Pseudomonas cichorii, Pseudomonas putida, Pseudomonas syringae and Serratia marcescens. The results reveal that the three genera of rhizobacteria were producers of hydrogen cyanide, indole acetic acid, catalase and solubilized phosphate. All Pseudomonas and Serratia isolates were producers of exopolysaccharides, protease and lipase while 80% of Bacillus strains were lipase producers and 60% produced exopolysaccharides and protease. As regards the production of ammonia by rhizobacteria, 100% by S. marcescens, 78% of Pseudomonas strains and 80% of Bacillus strains produce them. These results show the possibility of using these rhizobacteria as biological fertilizers to stimulate growth, control fungal diseases and improve crop productivity in Benin.

 

Key words: Rhizobacteria, Bacillus, Pseudomonas, Serratia, enzyme production, P-solubilizing bacteria, indole acetic acid (IAA).