African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4881

Full Length Research Paper

Metagenomic analysis of enteric bacterial pathogens affecting the performance of dairy cows in smallholder productions systems

Vincent Habimana
  • Vincent Habimana
  • Department of Animal Production, Faculty of Veterinary Medicine, University of Nairobi, Kenya.
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Rawlynce Cheruiyot Bett
  • Rawlynce Cheruiyot Bett
  • Department of Animal Production, Faculty of Veterinary Medicine, University of Nairobi, Kenya.
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Joshua Oluoch Amimo
  • Joshua Oluoch Amimo
  • Department of Animal Production, Faculty of Veterinary Medicine, University of Nairobi, Kenya.
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Felix Matura Kibegwa
  • Felix Matura Kibegwa
  • Department of Animal Production, Faculty of Veterinary Medicine, University of Nairobi, Kenya.
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Dedan Githae
  • Dedan Githae
  • Biosciences of East and Central Africa-International Livestock Research Institute (BecA-ILRI) Hub, Nairobi, Kenya.
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Joseph Owino Jung’a
  • Joseph Owino Jung’a
  • Department of Animal Production, Faculty of Veterinary Medicine, University of Nairobi, Kenya.
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  •  Received: 16 March 2018
  •  Accepted: 29 April 2018
  •  Published: 07 May 2018

Abstract

There is little information about the diversity of bacterial pathogens present in the rumen and feces of healthy cow and the subsequent effects on the performance of the host animal. The objectives of the present study were to genetically characterize the enteric bacterial pathogens found in the rumen fluid and cow feces and to identify the resistant genes responsible for antimicrobial resistance in the detected pathogens. The cow feces and rumen fluid samples (6 rumen fluid and 42 feces) were collected from lactating dairy cows. Using next generation sequencing, the enteric bacterial pathogens detected were screened for antimicrobial resistance genes using ResFinder-2.1 database in the center of Abricate. The characterized enteric bacterial pathogens include Escherichia coli, Salmonella enterica, Streptococcus agalactiae, Streptococcus pyogenes, Campylobacter coli, and Campylobacter fetus among others. Those enteric bacterial pathogens were also drug resistant bacteria except Campylobacter coli. The Campylobacter fetus fetus was identified as the only multidrug resistant bacterial pathogen detected in the cow feces. However, the abundant resistant genes detected confer resistance to tetracycline (17 genes from 209 contigs), beta-lactam (21 genes from 67 contigs), streptomycin (6 genes from 153 contigs), and sulfamethoxazole (2 genes from 72 contigs). This is the first study to identify the diversity of enteric bacterial pathogens from the station based and smallholder dairy cows in Kenya and Tanzania, respectively.

Key words: Antimicrobial resistant genes, enteric bacterial pathogens, dairy cows, next generation sequencing.