African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5090

Full Length Research Paper

Comparative evaluation of the antimicrobial profile of Moringa leaf and seed oil extracts against resistant strains of wound pathogens in orthopedic hospitals

Anthony C Mgbeahuruike
  • Anthony C Mgbeahuruike
  • Department of Microbiology, Faculty of Biological Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria.
  • Google Scholar
Gloria Edeh
  • Gloria Edeh
  • Department of Microbiology, Faculty of Biological Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria.
  • Google Scholar
Chuma S Eze
  • Chuma S Eze
  • Deparment of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria.
  • Google Scholar
Joshua Parker
  • Joshua Parker
  • Department of Applied Biology and Biotechnology, Enugu State University of Science and Technology, Enugu, Enugu State, Nigeria.
  • Google Scholar
Samuel Okezie Ekere
  • Samuel Okezie Ekere
  • Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria.
  • Google Scholar
Onyinye O Kanu
  • Onyinye O Kanu
  • Department of Veterinary Obstetrics and Gynecology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria.
  • Google Scholar
Esther Dibua
  • Esther Dibua
  • Department of Microbiology, Faculty of Biological Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria.
  • Google Scholar


  •  Received: 27 February 2016
  •  Accepted: 09 May 2016
  •  Published: 21 October 2017

Abstract

The antimicrobial profile of oil extracts from Moringa oleifera leaves and seeds on orthopaedic wound pathogens was tested and compared with the antimicrobial activity of some antibiotics. The pathogens were characterized using biochemical and morphological tests. Antimicrobial susceptibility test was done on the pathogens using paper discs diffusion method. Plasmid curing was done on the isolates that showed resistance to antibiotics and the Moringa extracts. Time of kill assay was done with modified plating technique. Staphylococcus aureus accounted for 43% of the pathogens followed by Proteus spp. (16%), Klebsiella spp. (15%), Citrobacter spp. (11%), Escherichia coli (8%) and Pseudomonas aeruginosa (6%). Over 80% of the organisms were resistant to the tested antibiotics and their resistance were of plasmid origin. The methanolic leaf oil extract showed highest antimicrobial activity. The activity of the aqueous seed oil extract was significantly higher than the methanolic and ethanolic seed oil extracts (P > 0.05). Viable cell counts of S. aureus and Klebsiella spp. were reduced by the methanolic leaf and aqueous seed oil extracts. The antimicrobial activity of the methanolic leaf and aqueous seed oil extracts compared favourably with the reference antibiotics and can be used as alternatives for treatment of orthopaedic wound infections.

Key words: Orthopeadic, antibiotics, plant extracts, plasmid, wound, infection, antimicrobial, Moringa oleifera.