African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5145

Full Length Research Paper

Isolation, screening and statistical optimizing of L-methioninase production by Chaetomium globosum

Shimaa R. Hamed
  • Shimaa R. Hamed
  • Microbial Biotechnology Department, National Research Centre, Dokki, 12622, Cairo, Egypt.
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Mostafa M. Abo Elsoud
  • Mostafa M. Abo Elsoud
  • Microbial Biotechnology Department, National Research Centre, Dokki, 12622, Cairo, Egypt.
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Manal G. Mahmoud
  • Manal G. Mahmoud
  • Microbial Biotechnology Department, National Research Centre, Dokki, 12622, Cairo, Egypt.
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Mohsen M. S. Asker
  • Mohsen M. S. Asker
  • Microbial Biotechnology Department, National Research Centre, Dokki, 12622, Cairo, Egypt.
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  •  Received: 28 May 2016
  •  Accepted: 16 August 2016
  •  Published: 28 September 2016

Abstract

Egyptian soil sample was screened for isolation methioninlytic fungi by a rapid plate assay procedure. Eighteen strains of different isolated fungi were screened quantitavely for their L-methioninase activity. Chaetomium globosum was the most efficacious isolate and a dematiaceous filamentous fungi, it was identified at the molecular level by ribotyping 18S rRNA along with the biochemical characterization, and lastly completed by BLAST analysis by structure of a phylogenetic tree. Results showed that, the optimum levels of the incubation period, temperature, pH, methionine, sucrose and sodium nitrate concentrations were 3 days, 30°C, 7, 0%, 30 g/l and 1g/l, respectively.  According to the produced model, at these levels, C. globosum produce L-methioninase with predicted specific activity of (≈2225 U/mg); so L-methioninase could be a good source for clinical therapeutic application. The rRNA sequence of C. globosum was deposited to gene bank under accession number KXO24450

 

Key words: L-methioninase, Chaetomium globosum, 18S rRNA, enzyme optimization, soil.