African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4889

Full Length Research Paper

Detection of Escherichia coli and total microbial population in River Siran water of Pakistan using Emb and Tpc agar

Sahibzada Waheed Abdullah
  • Sahibzada Waheed Abdullah
  • State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (LVRI, CAAS), Xujiaping I, Lanzhou, Gansu, 730046, The People’s Republic of China.
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Sahibzadi Aalia Gul
  • Sahibzadi Aalia Gul
  • Department of Microbiology, Hazara University, Mansehra, Khyber Pakhtunkhwa, Pakistan.
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Sana Farheen
  • Sana Farheen
  • Department of Microbiology, Hazara University, Mansehra, Khyber Pakhtunkhwa, Pakistan.
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Tayba Kibrea
  • Tayba Kibrea
  • Department of Microbiology, Hazara University, Mansehra, Khyber Pakhtunkhwa, Pakistan.
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Adila Saleem
  • Adila Saleem
  • Department of Microbiology, Hazara University, Mansehra, Khyber Pakhtunkhwa, Pakistan.
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Sahibzadi Sana Gul
  • Sahibzadi Sana Gul
  • Department of Zoology, Hazara University, Mansehra, Khyber Pakhtunkhwa, Pakistan.
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Umair Ahmad
  • Umair Ahmad
  • Department of Microbiology, Hazara University, Mansehra, Khyber Pakhtunkhwa, Pakistan.
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  •  Received: 04 August 2018
  •  Accepted: 11 October 2018
  •  Published: 14 October 2018

Abstract

There is an increased ratio of gastrointestinal diseases in the area of Mansehra, Pakistan, which depends on the Siran River for drinking. These diseases drew our attention to analyze the microbial population. For detection of Escherichia coli and other microbial population, samples were collected from different regions of Mansehra, Pakistan. Two samples were collected from each site for proper detection of microorganisms and repeated the process 5 times. The microbial counts were performed using total plate count agar (TPC) and eosin methylene blue (EMB). The samples were cultured on EMB and TPC agar at 370C for 24 h. The findings revealed that Baffa and Bajna had high microbial count confirmed by EMB and TPC. To this end, we confirmed that Siran River has high microbial count. The E. coli detection in water indicates the fecal contamination and the presence of E. coli in water makes the water unfit for drinking.

 

Key words: Escherichia coli, eosin methylene blue, total plate count, drinking water.