African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4875

Review

Value of matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry in clinical microbiology and infectious diseases in Africa and tropical areas

Christophe Flaudrops
  • Christophe Flaudrops
  • Aix-Marseille Université, Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, UM63, CNRS 7278, IRD 198, INSERM 1095, Marseille, France.
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Ngor Faye
  • Ngor Faye
  • Laboratory of General Parasitology, Cheikh Anta Diop University, Dakar, Senegal.
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Oleg Mediannikov
  • Oleg Mediannikov
  • Aix-Marseille Université, Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, UM63, CNRS 7278, IRD 198, INSERM 1095, Marseille, France.
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Cheikh Sokhna
  • Cheikh Sokhna
  • Aix-Marseille Université, Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, UM63, CNRS 7278, IRD 198, INSERM 1095, Marseille, France.
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Cheikh Ibrahima Lo
  • Cheikh Ibrahima Lo
  • Aix-Marseille Université, Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, UM63, CNRS 7278, IRD 198, INSERM 1095, Marseille, France.
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Bécaye Fall
  • Bécaye Fall
  • Hôpital Principal de Dakar, Senegal.
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Bissoume Sambe-Ba
  • Bissoume Sambe-Ba
  • Hôpital Principal de Dakar, Senegal.
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Boubacar Wade
  • Boubacar Wade
  • Hôpital Principal de Dakar, Senegal.
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Didier Raoult
  • Didier Raoult
  • Aix-Marseille Université, Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, UM63, CNRS 7278, IRD 198, INSERM 1095, Marseille, France.
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Florence Fenollar
  • Florence Fenollar
  • Aix-Marseille Université, Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, UM63, CNRS 7278, IRD 198, INSERM 1095, Marseille, France.
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  •  Received: 24 June 2017
  •  Accepted: 11 September 2017
  •  Published: 21 September 2017

Abstract

Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MALDI-TOF MS) is a revolutionary technique with multiple applications. Its use in clinical microbiology is now becoming widespread as the method is an easy, rapid, effective, accurate, and cheap way to identify cultured bacteria and fungi. It is, therefore, an ideal tool to replace conventional methods still used in Africa and tropical areas for routine microbiological diagnosis. The recent installation of a MALDI-TOF MS for diagnostic purposes in a hospital in Senegal has confirmed that this tool is not only valuable but also robust in tropical Africa, providing further evidence that this technique should be widely distributed there. However, despite its value for clinical microbiology in Africa, the acquisition and installation of MALDI-TOF MS is subject to several constraints. This review provides general information on aspects of MALDI-TOF MS. The specific aspects and constraints observed in Africa and tropical countries are also addressed with suggestions for appropriate solutions.

Key words: Microorganism, infectious diseases, quick identification, matrix-assisted laser desorption-ionization time-of-flight, matrix assisted laser desorption ionization-time of flight (MALDI-TOF).