African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5090

Full Length Research Paper

Cholesterol reduction in vitro by novel probiotic lactic acid bacterial strains of Enterococcus isolated from healthy infants’ stool

Akram A. Aboseidah
  • Akram A. Aboseidah
  • Department of Botany and Microbiology, Faculty of Science, Suez University, Suez, Egypt.
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Abdel-Hamied M. Rasmey
  • Abdel-Hamied M. Rasmey
  • Department of Botany and Microbiology, Faculty of Science, Suez University, Suez, Egypt.
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Magdy M. Osman
  • Magdy M. Osman
  • Department of Dairy, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt.
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Salha G. Desouky
  • Salha G. Desouky
  • Department of Botany and Microbiology, Faculty of Science, Suez University, Suez, Egypt.
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Nehal Kamal
  • Nehal Kamal
  • Department of Botany and Microbiology, Faculty of Science, Suez University, Suez, Egypt.
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  •  Received: 04 May 2017
  •  Accepted: 07 August 2017
  •  Published: 14 October 2017

Abstract

The present study aimed to isolate and evaluate the potentiality of some probiotic lactic acid bacteria from infants stool to degrade and reduce cholesterol level in fermentation medium. Out of 50 lactic acid bacterial isolates recovered from healthy infants (3 to 18 months old) stool, two bacterial isolates W7 and Y1 showed highest cholesterol reduction percentage (68.38 and 71.6%, respectively). The cholesterol reduction potentiality by the two isolates W7 and Y1 were optimized to 94 and 98%, respectively, by studying the different cultural conditions; inoculum size, inoculum age, pH, incubation temperature, incubation period and cholesterol concentration. The gas chromatography analysis of the fermentation extract revealed that the resulted end product of cholesterol was 5á-cholestane-3à,25-diol (C27H48O2) of molecular weight 404. The selected bacterial isolate Y1 was identified phenotypically and genotypically as Enterococcus faecium and deposited in GenBank under the accession number KY788356.

Key words: Cholesterol, Enterococcus, optimization, probiotic, gas chromatography.