African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5124

Full Length Research Paper

Biofilm, protease and lipase properties and antibiotic resistance profiles of staphylococci isolated from various foods

  Neslihan Gundogan* and Ozlem Ataol
  Department of Biology, Faculty of Science, Gazi University, Teknikokullar, Ankara 06500, Turkey.  
Email: [email protected]

  •  Accepted: 05 July 2013
  •  Published: 12 July 2013

Abstract

 

In this study, a total of 209 isolates were isolated from raw calf meat (minced), chicken drumsticks, raw milk, ice cream and white cheese samples. One hundred and seventy-five (83.7 %) coagulase-negative Staphylococci (CNS) and 34 (16.3%) coagulase-positive staphylococci (CPS) were isolated from these samples. The majority of Staphylococcusisolates showed biofilm formation (75.1%) and slime formation (68.4%). The frequency of positive protease and lipase production for Staphylococcus aureus isolates were 23.5 and 11.8%, respectively. Proteolytic and lipolytic activitiy were not found in the otherStaphylococcus species. The overall antimicrobial susceptibility profiles revealed that the highest percentage of resistance was detected for ampicillin (33.8%), tetracycline (26.3%), erythromycin (20.6%), methicillin (17.2%) and gentamicin (12.4%). The susceptibility to vancomycin, chloramphenicol, amikacin and clindamycin were 100% for all isolates.

 

Key words: Coagulase positive, coagulase negative Staphylococcus, biofilm, slime, lipase, protease, antibiotic resistance, foods