African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5236

Full Length Research Paper

Selection of high organic chromium yielding yeast strain after space piggyback experiment and its characteristic

Liu Lu1, Lv Jia-ping2* and Gao Yan-hong1,2
1Institute of Agro-Food Science and Technology, Chinese Academy of Agricultural Science (CAAS),  100193 Beijing PR China. 2College of Food Science and Engineering, Inner Mongolia Agricultural University, 010018 Huhhot PR China.
Email: [email protected]

  •  Accepted: 18 November 2011
  •  Published: 09 January 2012

Abstract

A high yield yeast strain with glucose tolerance factor (GTF) was screened from seven original yeast strains and seven strains mutated by spaceflight-induced mutagenesis through gradient concentration of chromium (Cr) plate cultivation and liquid fermentation cultivation with biomass of 40 g/L. By extracting glucose tolerance factor with ammonia and determining the content of Cr with flame atomic absorption spectrometry, the content of organic Cr was found to be 1296 µg/g and that of total Cr was 1926 µg/g. The dynamic relationship between other parameters and the amount of organic chromium absorbed by the yeast was also determined during fermentation.

Key words: Spaceflight-induced mutagenesis, glucose tolerance factor, glucose tolerance factor (GTF), organic chromium.