African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5045

Full Length Research Paper

The effects of bifidobacterium lactis and galactooligosaccharide (GOS) on ileum and distal colon motility: In vitro study

Nevcihan Gursoy
Department of Food Engineering, Faculty of Engineering, Cumhuriyet University, Sivas, TR-58140, Turkey.
Email: [email protected], [email protected]

  •  Accepted: 13 September 2011
  •  Published: 30 December 2011

Abstract

 

Twenty one male Wistar albino rats each weighing approximately 280 g were used in this study. Animals were divided into three groups. The first group (n = 7) consisted of sham controls, in the second (n = 7), rats were administrated 0.1 g/1 ml/ galactooligosaccharide by by oral gavage for 4 weeks. In the third group (n = 7), rats were administrated 109CFU/1 ml/day Bifidobacterium lactis by oral gavage for 4 weeks. After 4 weeks, rats were sacrified; ileum and proximal colon segments were removed. The spontaneous contractions of ileum and proximal colon were evaluated by using organ bath. It has been detected that both prebiotics and probiotics increased intestinal motility. While probiotics have effects on both ileum and proximal colon, prebiotics seem to be effective in colon. All data are expressed as mean ± SEM (standard error of mean). Statistical comparisons between groups were performed using general linear models of analysis of variance (ANOVA) followed by the Turkey test.

 

Key words: Bifidobacterium lactis, galactooligosaccharide, ileum, rat, distal colon, in vitro

Abbreviation

GOS, galactooligosaccharide; KBS, Krebsbicarbonate solution; CFU,colony forming units; NDO, non-digestible oligosaccharides; NaH2PO4, sodiumphosphate; NaCl, sodium chloride; KCl, potassium  chloride; CaCl2, calcium chloride;MgCl2, magnesium chloride; NaHCO3, sodium bicarbonate; SEM, standart error of mean; ANOVA, analysis of variance.