African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5182

Full Length Research Paper

Cyclic depsipeptide producing fluorescent pseudomonads exerts antifungal activity against fungal pathogens of maize (Zea mays)

Radhajeyalakshmi Raju
  • Radhajeyalakshmi Raju
  • Maize Research Station, Vagarai-624613, Palani, Tamil Nadu Agricultural University, Tamil Nadu, India.
  • Google Scholar
Sethuraman Kandhasamy
  • Sethuraman Kandhasamy
  • Maize Research Station, Vagarai-624613, Palani, Tamil Nadu Agricultural University, Tamil Nadu, India.
  • Google Scholar
Ganesan Kalipatty Nalliappan
  • Ganesan Kalipatty Nalliappan
  • Agricultural Research Station, Bhavanisagar-638451, Tamil Nadu Agricultural University, Tamil Nadu, India.
  • Google Scholar
Kumari Vinodhana Natarajan
  • Kumari Vinodhana Natarajan
  • Maize Research Station, Vagarai-624613, Palani, Tamil Nadu Agricultural University, Tamil Nadu, India.
  • Google Scholar
Karthikeyan Gandhi
  • Karthikeyan Gandhi
  • Department of Plant Pathology, Agricultural College and Research Institute, Madurai-625104, Tamil Nadu Agricultural University Tamil Nadu, India.
  • Google Scholar
Bharathi Chandrasekaran
  • Bharathi Chandrasekaran
  • Department of Soil Science and Agricultural Chemistry, Directorate of Natural Resource Management, Tamil Nadu Agricultural University, Coimbatore - 641 003, Tamil Nadu, India.
  • Google Scholar


  •  Received: 09 August 2016
  •  Accepted: 10 October 2016
  •  Published: 14 November 2016

Abstract

Cyclic lipopeptides (CLPs) with antibiotic, biosurfactant producing fluorescent pseudomonads were isolated from sugar beet-maize intercropped in sandy loam soils at Maize Research Station, Vagarai, TNAU. Approximately 20 fluorescent pseudomonads from sandy loam soils were isolated by using two different growth media. The strains were distinguished based on their growth, CFU/g, fluorescence, and pigment production. Growth inhibition of maize pathogens by CLP producing fluorescent pseudomonads strains were studied by dual culture experiments. The impact of CLP producing flurorescent pseudomonads strain on the zoospores of Downy mildew pathogen of maize was studied by direct microscopy and encysted zoospores were observed. Invitro, biochemical experiments confirmed the presence of Viscosinamide producing strain among the fluorescent pseudomonads isolates in terms of utilization of C and N sources. The particular strain was tested for its growth promoting activity by treating the maize seeds for their germination, and seedling vigour performance. Fluorescent pseudomonads can be affiliated to group under CLP producing biotypes/biovars. Purification of CLP (Viscosinamide) and characterization by HPLC analysis was carried out. Pot culture experiments were conducted to test the performance of CLP producing Pf strains in maize crop for testing their disease resistance. These biovars with antibiotic properties are the potential targets for the disease management in maize. CLPs in general receive considerable attention as potent antimicrobial drugs.

 

Key words: Cyclic lipopeptides, fluorescent pseudomonads, viscosinamide, zoospores, antifungal.