African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5166

Full Length Research Paper

Molecular characterization of high-risk infection vaginal bacteria isolated from pregnant women in CHU-MEL of Cotonou (Benin)

Haziz Sina
  • Haziz Sina
  • Department of Biochemistry and Cellular Biology, Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Sciences and Techniques, University of Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
  • Google Scholar
Christine N’tcha
  • Christine N’tcha
  • Department of Biochemistry and Cellular Biology, Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Sciences and Techniques, University of Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
  • Google Scholar
Durand Dah-Nouvlessounon
  • Durand Dah-Nouvlessounon
  • Department of Biochemistry and Cellular Biology, Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Sciences and Techniques, University of Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
  • Google Scholar
Gnimdou Gnama-Tchao
  • Gnimdou Gnama-Tchao
  • Department of Biochemistry and Cellular Biology, Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Sciences and Techniques, University of Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
  • Google Scholar
Bawa Boya
  • Bawa Boya
  • Department of Biochemistry and Cellular Biology, Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Sciences and Techniques, University of Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
  • Google Scholar
Akim Socohou
  • Akim Socohou
  • Department of Biochemistry and Cellular Biology, Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Sciences and Techniques, University of Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
  • Google Scholar
Abdou Ramanou Alao Sanni
  • Abdou Ramanou Alao Sanni
  • Bacteriology Laboratory, Lagune Mother and Child Hospital and University Center, Cotonou, Benin.
  • Google Scholar
Farid Baba-Moussa
  • Farid Baba-Moussa
  • Laboratory of Microbiology and Food Technology, Department of Plant Biology, Cotonou Benin, University of Abomey-Calavi, Benin.
  • Google Scholar
Adolphe Adjanohoun
  • Adolphe Adjanohoun
  • National Institute of Agronomic Research of Benin (INRAB), Cotonou, Benin.
  • Google Scholar
Lamine Baba-Moussa
  • Lamine Baba-Moussa
  • Department of Biochemistry and Cellular Biology, Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Sciences and Techniques, University of Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
  • Google Scholar


  •  Received: 17 October 2021
  •  Accepted: 29 November 2021
  •  Published: 31 December 2021

References

Agossou J, Hounnou-d'Almeida M, Noudamadjo A, Adédémy JD, Nékoua WS, Ayivi B (2016). Neonatal Bacterial Infections in Parakou in 2013. Open Journal of Pediatrics 6(1):100-108.
Crossref

 

Akbarian RZ, Haghshenas MM, Esmaeilzadeh S, Firouzjahi A, Laegh M, Khafri S, Naeimi Rad M (2016) Colonization of rectovaginal Escherichia coli and group B streptococci in mothers and on infants' body surface and their related risk factors. Caspian Journal of Pediatrics 2(2):148-152.

 

Alkeskas A, Ogrodzki P, Saad M, Masood N, Rhoma NR, Moore K, Farbos A, Paszkiewicz K, Forsythe S (2015). The molecular characterization of Escherichia coli K1 isolated from neonatal nasogastric feeding tubes." BMC infectious diseases 15(1):1-14.
Crossref

 

Anago E, Ayi-Fanou L, Akpovi CD, Hounkpe WB, Tchibozo MAD, Bankole HS, Sanni A (2015). Antibiotic resistance and genotype of beta-lactamase producing Escherichia coli in nosocomial infections in Cotonou, Benin. Annals of clinical microbiology and antimicrobials 14(1):1-6.
Crossref

 

Aranishi F, Okimoto T, Ohkubo M (2006). A short-cut DNA extraction from cod caviar. Journal of the Science of Food and Agriculture 86(3):425-428.
Crossref

 

Arora N, Sadovsky Y, Dermody TS, Coyne CB (2017). Microbial Vertical Transmission during Human Pregnancy. Cell host & microbe 21(5):561-567.
Crossref

 

Balaka B, Agbere A, Dagnra A, Baeta S, Kessie K, Assimadi K (2005). Genital bacterial carriage during the last trimester of pregnancy and early-onset neonatal sepsis. organe officiel de la Societe francaise de pediatrie 12(5):514-519.
Crossref

 

Bingen E, Picard B, Brahimi N, Mathy S, Desjardins P, Elion J, Denamur E (1998). Phylogenetic analysis of Escherichia coli strains causing neonatal meningitis suggests horizontal gene transfer from a predominant pool of highly virulent B2 group strains. The Journal of infectious diseases 177(3):642-650.
Crossref

 

Bolukaoto JY, Monyama CM, Chukwu MO, Lekala SM, Nchabeleng M, Maloba MRB, Mavenyengwa RT, Lebelo SL, Monokoane ST, Tshepuwane C, Moyo SR (2015). Antibiotic resistance of Streptococcus agalactiae isolated from pregnant women in Garankuwa, South Africa. BMC research notes 8(1):364.
Crossref

 

Brochet M, Couvé E, Bercion R, Sire JM, Glaser P (2009). Population structure of human isolates of Streptococcus agalactiae from Dakar and Bangui. Journal of clinical microbiology 47(3):800-803.
Crossref

 

CASFM (2020). "Comité de l'antibiogramme de la Société Française de Microbiologie : Recommandations 2020 V.1.1," 181 p, 2020.

View

 

Da Cunha V, Davies MR, Douarre PE, Rosinski-Chupin I, Margarit I, Spinali S, Perkins T, Lechat P, Dmytruk N, Sauvage E (2014). Streptococcus agalactiae clones infecting humans were selected and fixed through the extensive use of tetracycline. Nature communications 5:4544.
Crossref

 

Denis F, Cattoir V, Martin C, Ploy MC, Poyart C (2016). Bactériologie Médicale?: Techniques Usuelles. Elsevier Masson.

 

Edmond T, Malick ZF, Yehouenou LC, Bankolé HS, Wilfried BK, Marius EA, Ndiaye HD, Kane CT (2017). Bacterial distribution and antibiotic susceptibility pattern of Group B Streptococcus β hemolytic (GBS) in vaginal infections at Cotonou in Benin." American Journal of Infectious Diseases and Microbiology 5(3):109:114.
Crossref

 

Germon P, Chen YH, He L, Blanco JE, Bree A, Schouler C, Huang SH, Moulin-Schouleur M (2005). IbeA, a virulence factor of avian pathogenic Escherichia coli. Microbiology 151(4):1179-1186.
Crossref

 

Ghanbari F, Ghajavand H, Havaei R, Jami MS, Khademi F, Heydari L, Shahin M, Havaei A (2016). Distribution of erm genes among Staphylococcus aureus isolates with inducible resistance to clindamycin in Isfahan, Iran. Advanced biomedical research 22(5):62.
Crossref

 

Gizachew M, Tiruneh M, Moges F, Tessema B (2019). Streptococcus agalactiae maternal colonization, antibiotic resistance and serotype profiles in Africa: A meta-analysis. Annals of clinical microbiology and antimicrobials 18(1):14.
Crossref

 

Guo D, Xi Y, Wang S, Wang S (2019). Is a positive Christie-Atkinson-Munch-Peterson (CAMP) test sensitive enough for the identification of Streptococcus agalactiae? BMC infectious diseases 19(1):1-5.
Crossref

 

Gygax SE, Schuyler JA, Kimmel LE, Trama JP, Mordechai E, Adelson ME (2006). Erythromycin and clindamycin resistance in group B streptococcal clinical isolates. Antimicrobial agents and chemotherapy 50(5):1875-1877.
Crossref

 

Hays C, Louis M, Plainvert C, Dmytruk N, Touak G, Trieu-Cuot P, Poyart C, Tazi A (2016). Changing epidemiology of group B Streptococcus susceptibility to fluoroquinolones and aminoglycosides in France. Antimicrobial agents and chemotherapy 60(12):7424-7430.
Crossref

 

Huang SH, Chen YH, Kong G, Chen SH, Besemer J, Borodovsky M, Jong A (2001). A novel genetic island of meningitic Escherichia coli K1 containing the ibeA invasion gene (GimA): Functional annotation and carbon-source-regulated invasion of human brain microvascular endothelial cells. Functional & integrative genomics 1(5):312?322.
Crossref

 

Huang SH, Jong AY (2001). Cellular mechanisms of microbial proteins contributing to invasion of the blood-brain barrier: Micro Review. Cellular microbiology 3(5):277-287.
Crossref

 

Iregbu KC, Zubair KO, Modibbo IF, Aigbe AI, Sonibare SA, Ayoola OM (2013). Neonatal infections caused by Escherichia coli at the National Hospital, Abuja: A three-year retrospective study. African Journal of Clinical and Experimental Microbiology 14(2):95-100.
Crossref

 

Isaacs D (2005). Neonatal sepsis: The antibiotic crisis. Indian Pediatrics 42(1):9.

 

Kaczmarek A, Budzy?ska A, Gospodarek E (2012). Prevalence of genes encoding virulence factors among Escherichia coli with K1 antigen and non-K1 E. coli strains. Journal of medical microbiology 61(10):1360-1365.
Crossref

 

Kardos S, Tóthpál A, Laub K, Kristóf K, Ostorházi E, Rozgonyi F, Dobay O (2019). High prevalence of group B streptococcus ST17 hypervirulent clone among non-pregnant patients from a Hungarian venereology clinic. BMC infectious diseases 19(1):1-10.
Crossref

 

Kassebaum NJ, Bertozzi-Villa A, Coggeshall MS, Shackelford KA, Steiner C, Heuton KR, Gonzalez-Medina D, Barber R, Huynh C, Dicker D, Templin T, Wolock TM, Ozgoren AA, Abd-Allah F, Abera SF, Abubakar I, Achoki T, Adelekan A, Ademi Z, Adou AK, Adsuar JC, Agardh EE, Akena D, Alasfoor D, Alemu ZA, Alfonso-Cristancho R, Alhabib S, Ali R, Al Kahbouri MJ, Alla F, et al (2013). Global, regional, and national levels and causes of maternal mortality during 1990-2013: a systematic analysis for the Global Burden of Disease Study 2013. The Lancet 384(9947):980-1004.

 

Kataja J, Huovinen P, Seppälä H (2000). The Macrolide Resistance Study Group, Erythromycin resistance genes in group A streptococci of different geographical origins." Journal of Antimicrobial Chemotherapy 46(5):789?792.
Crossref

 

Kim KS, Itabashi H, Gemski P, Sadoff J, Warren RL, Cross AS (1992). The K1 capsule is the critical determinant in the development of Escherichia coli meningitis in the rat. The Journal of clinical investigation 90(3):897-905.
Crossref

 

Kiwanuka J, Bazira J, Mwanga J, Tumusiime D, Nyesigire E, Lwanga N, Warf BC, Kapur V, Poss M, Schiff SJ (2013). The Microbial Spectrum of Neonatal Sepsis in Uganda: Recovery of Culturable Bacteria in Mother-Infant Pairs. PlosOne 8(8):e72775.
Crossref

 

Leclercq R (2002). Mechanisms of resistance to macrolides and lincosamides: Nature of the resistance elements and their clinical implications. Clinical infectious diseases 34(4):482-492.
Crossref

 

Lekala LM, Mavenyengwa RT, Moyo SR, Lebelo SL, Bolukaoto JY, Chukwu MO, Monyama CM, Nchabeleng M, Tshepuwane TC, Monokoane S (2015). Risk factors associated with group B streptococcus colonization and their effect on pregnancy outcome." Journal of Gynecology and Obstetrics 3(6):121-128.

 

Madrid L, Maculuve SA, Vilajeliu A, Sáez E, Massora S, Cossa A, Varo R, Sitoe A, Mosqueda N, Anselmo R, Munguambe K, Soto SM, Moraleda C, Macete E, Menéndez C, Bassat Q (2018). Maternal Carriage of Group B Streptococcus and Escherichia coli in a District Hospital in Mozambique. The Pediatric infectious disease journal 37(11):1145-1153.
Crossref

 

Madrid L, Seale AC, Kohli-Lynch M (2017). Infant GBS Disease Investigator Group. Infant group B streptococcal disease incidence and serotypes worldwide: Systematic review and meta-analyses. Clinical infectious diseases 65(2): S160-S172.
Crossref

 

Megli CJ, Coyne CB (2021). Infections at the maternal-fetal interface: an overview of pathogenesis and defence. Nature Reviews Microbiology 1-16.
Crossref

 

Mengist A, Kannan H, Abdissa A (2016). Prevalence and antimicrobial susceptibility pattern of anorectal and vaginal group B Streptococci isolates among pregnant women in Jimma, Ethiopia. BMC research notes 9(1):351.
Crossref

 

Metcalf BJ, Chochua S, Gertz JRE, Hawkins PA, Ricaldi J, Li Z, Walker H, Tran T, Rivers J, Mathis S (2017). Short-read whole genome sequencing for determination of antimicrobial resistance mechanisms and capsular serotypes of current invasive Streptococcus agalactiae recovered in the USA. Clinical Microbiology and Infection 23(8):574-e7.
Crossref

 

Mohammed M, Asrat D, Woldeamanuel Y, Demissie A (2012). Prevalence of group B Streptococcus colonization among pregnant women attending antenatal clinic of Hawassa Health Center, Hawassa, Ethiopia. Ethiopian Journal of Health Development 26(1):36-42.

 

Moosavian M, Shoja S, Rostami S, Torabipour M, Farshadzadeh Z (2014). Inducible clindamycin resistance in clinical isolates of Staphylococcus aureus due to erm genes, Iran. Iranian journal of microbiology 6(6):421.

 

Moulin-Schouleur M, Schouler C, Tailliez P, Kao MR, Brée A, Germon P, Oswald E, Mainil J, Blanco M, Blanco J (2006). Common virulence factors and genetic relationships between O18: K1: H7 Escherichia coli isolates of human and avian origin. Journal of clinical microbiology 44(10):3484-3492.
Crossref

 

Moussé W, Sina H, Mama-Sirou IA, Anago E, Dah-Nouvlessounon D, N'Tcha C, Bade F, Anagonou S, Adjanohoun A, Baba-Moussa L (2019). Antibiotic Resistance and Production of Extended Spectrum β-Lactamases by Clinical Gram-negative Bacteria in Benin. Journal of Advances in Microbiology pp. 1-13.
Crossref

 

Mugalu J, Nakakeeto MK, Kiguli S, Kaddu-Mulindwa DH (2006). Etiology, risk factors and immediate outcome of bacteriologically confirmed neonatal septicemia in Mulago hospital, Uganda. African health sciences 6(2):120-126.

 

Ngoulou BT, Ahombo G, Nguimbi E, Ampa RR, Moyen R (2019). Caractérisation moléculaire et distribution des gènes codant la résistance aux macrolides, lincosamides et streptogramines b chez staphylococcus communautaires et cliniques à Brazzaville, Congo. Afrique Science 15(5):352-363.

 

Ogunlesi TA, Ogunfowora OB, Osinupebi O, Olanrewaju DM (2011). Changing trends in newborn sepsis in Sagamu, Nigeria: Bacterial etiology, risk factors and antibiotic susceptibility. Journal of paediatrics and child health 47(1?2):5-11.
Crossref

 

Okinda N, Mulwa E, Revathi G (2014). a five-year review of API20e bacteria identification system's performance at a teaching hospital. East African medical Journal 91(3):73-76.

 

Okomo U, Akpalu ENK, Le Doare K, Roca A, Cousens S, Jarde A, Sharland M, Kampmann B, Lawn JE (2019). Etiology of invasive bacterial infection and antimicrobial resistance in neonates in sub-Saharan Africa: A systematic review and meta-analysis in line with the STROBE-NI reporting guidelines." The Lancet Infectious diseases 19(1):1219?1234.
Crossref

 

Ott M, Hacker J, Schmoll T, Jarchau T, Korhonen TK, Goebel W (1986). Analysis of the genetic determinants coding for the S-fimbrial adhesin (sfa) in different Escherichia coli strains causing meningitis or urinary tract infections. Infection and immunity 54(3):646-653.
Crossref

 

Poyart C, Réglier-Poupet H, Tazi A, Billoët A, Dmytruk N, Bidet P, Bingen E, Raymond J, Trieu-Cuot P (2008). Invasive group B streptococcal infections in infants, France. Emerging infectious diseases 14(10):1647.
Crossref

 

Quincampoix JC, Mainardi JL (2001). Mécanismes de résistance des cocci à Gram positif. Resuscitation 10(3):267-275.
Crossref

 

Rani V, Mehra R, Gupta V, Huria A, Chander J (2014). Vaginal flora in preterm premature rupture of membranes and their sensitivity to commonly used antibiotics. Asian Journal of Medical Sciences 5(4):58-60.
Crossref

 

Saderi H, Emadi B, Owlia P (2011). Phenotypic and genotypic study of macrolide, lincosamide and streptogramin B (MLSB) resistance in clinical isolates of Staphylococcus aureus in Tehran, Iran. Medical science monitor: international medical journal of experimental and clinical research 17(2):48-53.
Crossref

 

Saizonou J, Ouédraogo L, Paraiso M, Ayélo P, Kpozèhouen A, Daraté R, Traoré E (2014). Epidemiology and management of intrapartum infections in the maternity ward of Ouémé-Plateau County hospital in Benin. The Pan African Medical Journal 17:89-89.
Crossref

 

Salou M, Lack F, Adama-Hondegla A, Dossim S, Tsogbalé N, Gbadoé A, Akpadza K, Prince-David M (2015). Premature Rupture of the Membranes at the Sylvanus Olympio University Hospital of Lomé, Togo: Microbiological Findings and Outcomes. American Journal of Infectious Diseases and Microbiology 3(6):152-156.

 

Seppälä H, Skurnik M, Soini H, Roberts MC, Huovinen P (1998). A novel Erythromycin resistance methylase gene (ermTR) in Streptococcus pyogenes. Antimicrobial agents and chemotherapy 42(2):257-262.
Crossref

 

Shields P, Cathcart L (2010). Oxidase test protocol. American Society for Microbiology.

View

 

Simonsen KA, Anderson-Berry AL, Delair SF, Davies HD (2014). Early-onset neonatal sepsis. Clinical microbiology reviews 27(1):21-47.
Crossref

 

Six A, Joubrel C, Tazi A, Poyart C (2014). Infections materno-foetales à Streptococcus agalactiae. Presse Médicale 43(6):706-714.
Crossref

 

Sperber WH, Tatini SR (1975). Interpretation of the Tube Coagulase Test for Identification of Staphylococcus aureus. Applied microbiology 29(4):502?505.
Crossref

 

Srinivasan V, Metcalf BJ, Knipe KM, Ouattara M, McGee L, Shewmaker PL, Glennen A, Nichols M, Harris C, Brimmage M (2014). VanG element insertions within a conserved chromosomal site conferring vancomycin resistance to. Streptococcus agalactiae." mBio 5(4):e01386-14.
Crossref

 

Teatero S, Ramoutar E, McGeer A, Li A, Melano RG, Wasserscheid J, Dewar K, Fittipaldi N (2016). Clonal Complex 17 Group B Streptococcus strains causing invasive disease in neonates and adults originate from the same genetic pool. Scientific reports 6:20047. 
Crossref

 

Wang Y, Kim KS (2002). Role of OmpA and IbeB in Escherichia coli K1 invasion of brain microvascular endothelial cells in vitro and in vivo. Pediatric research 51(5):559-563.
Crossref

 

World Health Organization (WHO) (2019). "Liste OMS des antibiotiques d'importance critique pour la médecine humaine" (WHO/NMH/FOS/FZD/19.1) Article WHO/NMH/FOS/FZD/19.1. 2019. 

View

 

World Health Organization (WHO) (2016)."WHO recommendations on antenatal care for a positive pregnancy experience." World Health Organization, 172 p, 2016.

View

 

Zeng X, Kong F, Wang H, Darbar A, Gilbert GL (2006). Simultaneous detection of nine antibiotic resistance-related genes in Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization assay. Antimicrobial agents and chemotherapy 50(1):204-209.
Crossref

 

Zmantar T, Kouidhi B, Miladi H, Bakhrouf A (2011). Detection of macrolide and disinfectant resistance genes in clinical Staphylococcus aureus and coagulase-negative staphylococci." BMC research notes 4(1):453.
Crossref