African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5241

Full Length Research Paper

Antibiotic resistance and molecular characterization of Staphylococcus species from mastitic milk

Marjory Xavier Rodrigues*
  • Marjory Xavier Rodrigues*
  • Departament of Agroindustry, Food and Nutrition, Luiz de Queiroz College of Agriculture, University of São Paulo, 11 Padua Dias Ave. – Piracicaba, São Paulo, 13418-900, Brazil.
  • Google Scholar
Nathalia Cristina Cirone Silva
  • Nathalia Cristina Cirone Silva
  • Departament of Agroindustry, Food and Nutrition, Luiz de Queiroz College of Agriculture, University of São Paulo, 11 Padua Dias Ave. – Piracicaba, São Paulo, 13418-900, Brazil.
  • Google Scholar
Julia Hellmeister Trevilin
  • Julia Hellmeister Trevilin
  • Departament of Agroindustry, Food and Nutrition, Luiz de Queiroz College of Agriculture, University of São Paulo, 11 Padua Dias Ave. – Piracicaba, São Paulo, 13418-900, Brazil.
  • Google Scholar
Melina Mary Bravo Cruzado
  • Melina Mary Bravo Cruzado
  • Departament of Agroindustry, Food and Nutrition, Luiz de Queiroz College of Agriculture, University of São Paulo, 11 Padua Dias Ave. – Piracicaba, São Paulo, 13418-900, Brazil.
  • Google Scholar
Tsai Siu Mui
  • Tsai Siu Mui
  • Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture, University of São Paulo, 303 Centenário Ave. - Piracicaba - São Paulo, 13400-970, Brazil.
  • Google Scholar
Fabio Rodrigo Sanches Duarte
  • Fabio Rodrigo Sanches Duarte
  • Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture, University of São Paulo, 303 Centenário Ave. - Piracicaba - São Paulo, 13400-970, Brazil.
  • Google Scholar
Carmen J. Contreras Castillo1
  • Carmen J. Contreras Castillo1
  • Departament of Agroindustry, Food and Nutrition, Luiz de Queiroz College of Agriculture, University of São Paulo, 11 Padua Dias Ave. – Piracicaba, São Paulo, 13418-900, Brazil.
  • Google Scholar
Solange Guidolin Canniatti-Brazaca
  • Solange Guidolin Canniatti-Brazaca
  • Departament of Agroindustry, Food and Nutrition, Luiz de Queiroz College of Agriculture, University of São Paulo, 11 Padua Dias Ave. – Piracicaba, São Paulo, 13418-900, Brazil.
  • Google Scholar
Ernani Porto
  • Ernani Porto
  • Departament of Agroindustry, Food and Nutrition, Luiz de Queiroz College of Agriculture, University of São Paulo, 11 Padua Dias Ave. – Piracicaba, São Paulo, 13418-900, Brazil.
  • Google Scholar


  •  Received: 23 October 2016
  •  Accepted: 30 November 2016
  •  Published: 21 January 2017

References

Aarestrup FM, Dangler CA, Sordillo LM (1995). Prevalence of coagulase gene polymorphism in Staphylococcus aureus isolates causing bovine mastitis. Can. J. Vet. Res. 59(2):124-128.

 

Aires-de-Sousa M, Parente CESR, Vieira-da-Motta O, Bonna ICF, Silva DA, Lencastre H (2007). Characterization of Staphylococcus aureus isolates from buffalo, bovine, ovine, and caprine milk samples collected in Rio de Janeiro State, Brazil. Appl. Environ. Microb. 73(12):3845-3849.
Crossref

 

Argudín MA, Mendonza MC, Rodicio MR (2010). Food poisoning and Staphylococcus aureus enterotoxins. Toxins (Basel) 2(7):1751-1773.
Crossref

 

Bhutia KO, Singh TS, Biswas S, Adhikari L (2012). Evaluation of phenotypic with genotypic methods for species identification and detection of methicillin resistant in Staphylococcus aureus. Int. J. Appl. Basic Med. Res. 2(2):84-91.
Crossref

 

Clinical Laboratory Standards Institute (2015). Performance Standards for Antimicrobial Susceptibility Testing. Wayne (PA): Clinical and Laboratory Standards Institute/NCCLS; 2015 [Twenty-fifth Informational Supplement, M100–S25].

 

Cremonesi P, Luzzana M, Brasca M, Morandi S, Lodi R, Agnellini D, Caramenti G, Moroni P, Castiglioni B (2005). Development of a multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products. Mol. Cell Probes 19(5):299-305.
Crossref

 

Cuny C, Layer F, Strommenger B, Witte W (2011). Rare Occurrence of Methicillin-Resistant Staphylococcus aureus CC130 with a Novel mecA Homologue in Humans in Germany. PLoS One 6:1-5.
Crossref

 

Dordet-Frisoni E, Dorchies G, de Araujo C, Talon R, Leroy S (2007). Genomic Diversity in Staphylococcus xylosus. Appl. Environ. Microbiol. 73(22):7199-7209.
Crossref

 

Fischer A, Francois P, Holtfreter S, Broeker B, Schrenzel J (2009). Development and evaluation of a rapid strategy to determine enterotoxin gene content in Staphylococcus aureus. J. Microbiol. Methods 77(2):184-190.
Crossref

 

Giannechini R, Concha C, Rivero R, Delucci I, López MJ (2002). Occurrence of clinical and sub-clinical mastitis in dairy herds in the west littoral region in Uruguay. Acta Vet. Scand. 43(4):221-230.
Crossref

 

Gómez-Sanz E, Torres C, Lozano C, Fernández-Pérez R, Aspiroz C, Ruiz-Larrea F, Zarazaga M (2010). Detection, molecular characterization, and clonal diversity of methicillin-resistant Staphylococcus aureus CC398 and CC97 in Spanish slaughter pigs of different age groups. Foodborne Pathog. Dis. 7(10):1269-1277.
Crossref

 

Hu DL, Nakane A (2014). Mechanisms of staphylococcal enterotoxins-induced emesis. Eur. J. Pharmacol. 722:95-107.
Crossref

 

Jarraud S, Mougel C, Thioulouse J, Lina G, Meugnier H, Forey F, Nesme X, Etienne J, Vandensesch F (2002). Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (alleles), and human disease. Infect. Immun. 70(2):631-641.
Crossref

 

Johnson WM, Tyler SD, Ewan EP, Ashton FE, Pollard DR, Rozee KR (1991). Detection of genes for enterotoxins, exfoliative toxins, and toxic shock syndrome toxin 1 in Staphylococcus aureus by the polymerase chain reaction. J. Clin. Microbiol. 29(3):426-430.

 

Jorgensen HJ, Mathisen T, Lovseth A, Omoe K, Qvale KS, Loncarevic S (2005). An outbreak of Staphylococcus food poisoning cause by enterotoxin H in mashed potato made with raw milk. FEMS Microbiol. Lett. 252(2):267-272.
Crossref

 

Kondo Y, Ito T, Ma XX, Watanabe S, Kreiswirth BN, Etienne J, Hiramatsu K (2007). Combination of multiplex PCRs for Staphylococcal Cassette Chromosome mec type assignment: rapid identification system for mec, ccr, and major differences in junkyard regions. Antimicrob. Agents Chemother. 51(1):264-274.
Crossref

 

Lange CC, Brito MAVP, Reis DRL, Machado MA, Guimarães AS, Azevedo AL, Salles ÉB, Alvim MC, Silva FS, Meurer IR (2015). Species-level identification of staphylococci isolated from bovine mastitis in Brazil using partial 16S rRNA sequencing. Vet. Microbiol. 176(3-4):382-388.
Crossref

 

Lina G, Piémont Y, Godail-Gamot F, Bes M, Peter MO, Gauduchon V, Vandenesch F, Etienne J (1999). Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin. Infect. Dis. 29(5):1128-1132.
Crossref

 

Mehrotra M, Wang G, Johnson WM (2000). Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance. J. Clin. Microbiol. 38(3):1032-1035.

 

Moon JS, Lee AR, Kang HM, Lee ES, Kim MN, Paik YH, Joo YS, Koo HC (2007). Phenotypic and genetic antibiogram of methicillin-resistant staphylococci isolated from bovine mastitis in Korea. J. Dairy Sci. 90(3):1176-1185.
Crossref

 

Morandi S, Brasca M, Andrighetto C, Lombardi A, Lodi R (2009). Phenotypic and genotypic characterization of Staphylococcus aureus strains from Italian dairy product. Int. J. Food Microbiol. 2009:1-7.
Crossref

 

Nashev D, Toshkova K, Salasia SI, Hassan AA, Lämmler C, Zschöck M (2004). Distribution of virulence genes of Staphylococcus aureus isolated from stable nasal carriers. FEMS Microbiol. Lett. 233(1):45-52.
Crossref

 

Omoe K, Hu DL, Takahashi-Omoe H, Nakane A, Shinagawa K (2005). Comprehensive analysis of classical and newly described staphylococcal superantigenic toxin genes in Staphylococcus aureus isolates. FEMS Microbiol. Lett. 246(2):191-198.
Crossref

 

Omoe K, Ishikama M, Shimoda Y, Hu DL, Ueda S, Shinagawa K (2002). Detection of seg, seh and sei genes in Staphylococcus aureus isolates and determination of the enterotoxin productivities of S. aureus isolate harboring seg, seh or sei genes. J. Clin. Microbiol. 40(3):857-862.
Crossref

 

Ono HK, Omoe K, Imanishi K, Iwakabe Y, Hu DL, Kato H, Saito N, Nakane A, Uchiyama T, Shinagawa K (2008). Identification and characterization of two novel staphylococcal enterotoxins, types S and T. Infect. Immun. 76(11):4999-5005.
Crossref

 

Ote I, Taminiau B, Duprez JN, Dizier I, Mainil JG (2011). Genotypic characterization by polymerase chain reaction of Staphylococcus aureus isolates associated with bovine mastitis. Vet. Microbiol. 153(3-4):285-292.
Crossref

 

Park JY, Fox LK, Seo KS, McGuire MA, Park YH, Rurangirwa FR, Sischo WM, Bohach GA (2011). Detection of classical and newly described staphylococcal superantigen genes in coagulase-negative staphylococci isolated from bovine intramammary infections. Vet. Microbiol. 147(1-2):149-154.
Crossref

 

Rajic-Savic N, Katic V, Velebit B, Colovic S (2015). Characteristics of Enterotoxigenic Coagulase Positive Staphylococci isolated from bovine milk in cases of subclinical mastitis. Procedia Food Sci. 5:250-253.
Crossref

 

Sasaki T, Tsubakishita S, Tanaka Y, Sakusabe A, Ohtsuka M, Hirotaki S, Kawakami T, Fukata T, Hiramatsu K (2010). Multiplex-PCR method species identification of coagulase-positive staphylococci. J. Clin. Microbiol. 48(3):765-769.
Crossref

 

Shopsin B, Mathema B, Alcabes P, Said-Salim B, Lina G, Matsuka, Martinez J, Kreiswirth BN (2003). Prevalence of agr specificity groups among Staphylococcus aureus strains colonizing children and their guardians. J. Clin. Microbiol. 41(1):456-459.
Crossref

 

Silva NCC, Guimarães FF, Manzi MP, Budri PE, Gómez-Sanz E, Benito D, Langoni H, Rall VL, Torres C (2013). Molecular characterization and clonal diversity of methicillin susceptible Staphylococcus aureus in milk of cows with mastitis in Brazil. J. Dairy Sci. 96(11):6856-6862.
Crossref

 

Silva NCC, Guimarães FF, Manzi MP, Gómez-Sanz E, Gómez P, Araújo-Júnior JP, Langoni H, Rall VL, Torres C (2014). Characterization of methicillin-resistant coagulase-negative staphylococci in milk from cows with mastitis in Brazil. Antonie van Leeuwenhoek 106(2):2270-233.
Crossref

 

Thomas D, Dauwalder O, Brun V, Badiou C, Ferry T, Etienne J, Vandenesch F, Lina G (2009). Staphylococcus aureus superantigens elicit redundant and extensive human Vbeta patterns. Infect. Immun. 77(5):2043-2050.
Crossref

 

van de Klundert, JAM, Vliegenthart JS (1993). PCR detection of genes for aminoglycoside-modifying enzymes. In. Persing, DH, Smith, TF, Tenover, FC, White, TJ. Diagnostic molecular microbiology. Washington. Am. Soc. Microbiol. 1993:547-552.

 

Wilson GJ, Seo KS, Cartwright RA, Connelley T, Chuang-Smith ON, Merriman JA, Guinane CM, Park JY, Bohach GA, Schlievert PM, Morrison WI, Fitzgerald JR (2011). A novel core genome-encoded superantigen contributes to lethality of community-associated MRSA necrotizing pneumonia. PLoS Pathogens 7:1-16.
Crossref

 

Yamaguchi T, Nishifuji K, Sasaki M, Fudaba Y, Aepfelbacher M, Takata T, Ohara M, Komatsuzawa H, Amagai M, Sugai M (2002). Identification of the Staphylococcus aureus etd pathogenicity island which encodes a novel exfoliative toxin, ETD, and EDIN-B. Infect. Immun. 70(10):5835-5845.
Crossref