Molecular characterization of carbapenem resistant Gram-negative rods in Neonatal Intensive Care Unit of Mansoura University Childrens Hospital

Heba Elsayed Eldegla; Islam Nour; Nehad Nasef; Basma Shouman; Hesham Abdel-Hady; Mayada Zeid; Nawal S. Gouda; Manal M. Nomir; Abd Elazeez Shabaan; Noha Mostafa Mahmoud

doi:10.5897/AJMR2021.9497

African Journal of
Microbiology Research

Abbreviation: Afr. J. Microbiol. Res.
Language: English
ISSN: 1996-0808
DOI: 10.5897/AJMR
Start Year: 2007
Published Articles: 5166

Full Length Research Paper

Molecular characterization of carbapenem resistant Gram-negative rods in Neonatal Intensive Care Unit of Mansoura University Children's Hospital

Heba Elsayed Eldegla

Heba Elsayed Eldegla
Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Search for this author on:
Google Scholar

Islam Nour

Islam Nour
Neonatal Intensive Care Unit, Mansoura University Children’s Hospital, Mansoura, Egypt.
Search for this author on:
Google Scholar

Nehad Nasef

Nehad Nasef
Neonatal Intensive Care Unit, Mansoura University Children’s Hospital, Mansoura, Egypt.
Search for this author on:
Google Scholar

Basma Shouman

Basma Shouman
Neonatal Intensive Care Unit, Mansoura University Children’s Hospital, Mansoura, Egypt.
Search for this author on:
Google Scholar

Hesham Abdel-Hady

Hesham Abdel-Hady
Neonatal Intensive Care Unit, Mansoura University Children’s Hospital, Mansoura, Egypt.
Search for this author on:
Google Scholar

Mayada Zeid

Mayada Zeid
Departement of Pediatrics, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Search for this author on:
Google Scholar

Nawal S. Gouda

Nawal S. Gouda
Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Search for this author on:
Google Scholar

Manal M. Nomir

Manal M. Nomir
Clinical Pathology, Mansoura University Student Hospital, Mansoura, Egypt.
Search for this author on:
Google Scholar

Abd Elazeez Shabaan

Abd Elazeez Shabaan
Neonatal Intensive Care Unit, Mansoura University Children’s Hospital, Mansoura, Egypt.
Search for this author on:
Google Scholar

Noha Mostafa Mahmoud

Noha Mostafa Mahmoud
Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Search for this author on:
Google Scholar

Article Number - 53DF9E666937
Vol.15(6), pp. 286-294 , June 2021
https://doi.org/10.5897/AJMR2021.9497

Received: 07 February 2021
Accepted: 13 May 2021
Published: 30 June 2021

References

Akova M, Daikos GL, Tzouvelekis L, Carmeli Y (2012). Interventional strategies and current clinical experience with carbapenemase-producing Gram-negative bacteria. Clinical Microbiology and Infection 18(5):439-448. Crossref

Al Naiemi N, Cohen Stuart J, Leverstein van Hall M (2012). NVMM Guideline Laboratory detection of highly resistant microorganisms (HRMO), version 2.0. 2012.

Albiger B, Glasner C, Struelens MJ, Grundmann H, Monnet DL (2015). European Survey of Carbapenemase-Producing Enterobacteriaceae Working Group. Carbapenemase-producing Enterobacteriaceae in Europe: assessment by national experts from 38 countries. Europe's Journal on Infectious Disease Surveillance, Epidemiology, Prevention and Control 20(45). Crossref

Al-Zahrani IA (2018). Routine detection of carbapenem-resistant Gram-negative bacilli in clinical laboratories: A review of current challenges. Saudi Medical Journal 39(9):861-872. Crossref

Baran I, Aksu N (2016). Phenotypic and genotypic characteristics of carbapenem-resistant Enterobacteriaceae in a tertiary-level reference hospital in Turkey. Annals Clinical Microbiology Antimicrobials 15:20. Crossref

Centers for Disease Control and Prevention (2014). Guidance for control of carbapenem-resistant Enterobacteriaceae. Centers for Disease Control and Prevention, Atlanta, GA. View.

Centers for Disease Control and Prevention (2013). Vital signs: carbapenem-resistant Enterobacteriaceae. Morbidity and Mortality Weekly Report (MMWR) 62:165-170.

Choudhury DD, Singh NP, Rai S, Batra P, Manchanda V (2018). Carbapenem resistant Enterobacteriaceae neonatal gut colonization: A future concern in healthcare settings. Indian Journal Microbiology Research 5(3):348-354. Crossref

Clinical and Laboratory Standards Institute, CLSI (2017). Performance standards for antimicrobial susceptibility testing: Twenty-seven informational supplements; document (M100- S27). Wayne, PA: CLSI.

Elbadawi HS, Elhag KM, Mahgoub E, Altayb HN, Ntoumi F, Elton L, McHugh TD, Tembo J, Ippolito G, Osman AY, Zumla A, Abdel Hamid MM (2021). Detection and characterization of carbapenem resistant Gram?negative bacilli isolates recovered from hospitalized patients at Soba University Hospital, Sudan. BMC Microbiology 21(1):1-9. Crossref

Endimiani A, Perez F, Bajaksouzian S, Windau AR, Good CE, Choudhary Y, Hujer AM, Bethel CR, Bonomo RA, Jacobs MR (2010). Evaluation of updated interpretative criteria for categorizing Klebsiella pneumoniae with reduced carbapenem susceptibility. Journal of Clinical Microbiology 48:4417-4425. Crossref

Fattouh M, Nasr El-din A, Omar MA (2015). Detection of Klebsiella pneumoniae Carbapenemase (KPC) Producing Gram Negative Superbugs: An Emerging Cause of Multidrug-Resistant Infections in General Surgery Department of Sohag University Hospital, Egypt. International Journal of Current Microbiology and Applied Science 4(5):1-15.

Joji RM, Al-Rashed N, Saeed NK, Bindayna KM (2019). Detection of VIM and NDM-1 metallo-beta-lactamase genes in carbapenem-resistant Pseudomonas aeruginosa clinical strains in Bahrain. Journal of Laboratory Physicians 11(2):138-143. Crossref

Karuniawati A, Saharman YR, Lestari DC (2013). Detection of carbapenemase encoding genes in Enterobacteriacea, Pseudomonas aeruginosa and Acinetobacter baumanii isolated from patients at Intensive Care Unit Cipto Mangunkusumo Hospital in 2011. Acta Medica Indonesiana 45:101-106.

Liao K, Chen Y, Huang H, Guo P, Chen D, Liu M, Zeng Y (2014). Molecular characteristics of carbapenem-resistant Enterobacteriaceae isolates from a Chinese Tertiary hospital in Guangdong. Journal of Microbiology and Infectious Diseases 4(4):145-151. Crossref

Mahon CR, Lehman DC, Manuselis G (2007/2008). Textbook of Diagnostic Microbiology 2007. 2008. 3rd ed. St. Louis, Mo: Saunders Elsevier.

Makharita RR, El-Kholy I, Hetta HF, Abdelaziz MH, Hagagy FI, Ahmed AA, Algammal AM (2020). Antibiogram and Genetic Characterization of Carbapenem-Resistant Gram-Negative Pathogens Incriminated in Healthcare-Associated Infections. Infection and Drug Resistance 13:3991-4002. Crossref

Metwally WS, Elnagar WM (2019). Multidrug Resistant Uropathogens among Egyptian Pregnant Women. American Journal of Infectious Diseases 15(4):115-122. Crossref

Moemen D, Masallat DT (2017). Prevalence and characterization of carbapenem-resistant Klebsiella pneumoniae isolated from intensive care units of Mansoura University hospitals. Egyptian Journal of Basic and Applied Science 4(1)37-41. Crossref

Mohamed T, Yousef LM, Darweesh EI, Khalil AK, Meghezel EM (2018). Detection and Characterization of Carbapenem Resistant Enterobacteriacea in Sohag University Hospitals. Egyptian Journal of Medical Microbiology 27(4):61-69.

Mzimela BW, Nkwanyana NM, Singh R (2021). Clinical outcome of neonates with Carbapenem-resistant Enterobacteriacea infections at the King Edward VII Hospital's neonatal unit, Durban, South Africa. Southern African Journal of Infectious Diseases 36(1):a223. Crossref

Netikul T, Kiratisin P (2015). Genetic Characterization of Carbapenem-Resistant Enterobacteriaceae and the Spread of Carbapenem Resistant Klebsiella pneumonia ST340 at a University Hospital in Thailand. PLOS ONE 10(9):e0139116. Crossref

Nordmann P, Gniadkowski M, Giske CG, Poirel L, Woodford N, Miriagou V (2012). Identification and screening of carbapenemase producing Enterobacteriaceae. Clinical Microbiology and Infection Journal 18(5):432-438. Crossref

Okoche D, Asiimwe BB, Katabazi FA, Kato L, Najjuka CF (2015). Prevalence and Characterization of Carbapenem-Resistant Enterobacteriaceae Isolated from Mulago National Referral Hospital, Uganda. PLOS ONE 10(8):e0135745. Crossref

Poirel L, Walsh TR, Cuvillier V, Nordmann P (2011). Multiplex PCR for detection of acquired carbapenemase genes. Diagnostic Microbiology and Infectious Disease Journal 70(1):119-123. Crossref

Pollett S, Miller S, Hindler J, Uslan D, Carvalho M, Humphries RM (2014). Phenotypic and molecular characteristics of carbapenem-resistant Enterobacteriaceae in a health care system in Los Angeles, California, from 2011 to 2013. Journal of Clinical Microbiology 52(11):4003-4009. Crossref

Tamma PD, Simner PJ (2018). Phenotypic Detection of Carbapenemase-Producing Organisms from Clinical Isolates. Journal of Clinical Microbiology 56(11):e01140-18. Crossref

Vading M, Samuelsen O, Haldorsen B, Sundsfjord AS, Giske CG (2011). Comparison of disk diffusion, Etest and VITEK2 for detection of carbapenemase-producing Klebsiella pneumoniae with the EUCAST and CLSI breakpoint systems. Clinical Microbiology and Infection Journal 17(5):668-674. Crossref

Viau RA, Hujer AM, Marshall SH, Perez F, Hujer KM, Briceno DF, Dul M, Jacobs MR, Grossberg R, Toltzis P, Bonomo RA (2012). "Silent" dissemination of Klebsiella pneumoniae isolates bearing K. pneumoniae carbapenemase in a long-term care facility for children and young adults in Northeast Ohio. Clinical Infectious Disease Journal 54(9):1314-1321. Crossref

Woodford N, Tierno PM , Young K, Tysall L, Palepou MFI, Ward E, Painter RE, Suber DF, Shungu D, Silver LL, Inglima K, Kornblum J, Livermore DM (2004). Outbreak of Klebsiella pneumoniae producing a new carbapenem-hydrolyzing class A b-lactamase, KPC-3, in a New York Medical Center. Antimicrobial Agents Chemotherapy 48(12):4793-4799. Crossref

Yang Y, Chen J, Lin D, Xu X, Cheng J, Sun C (2018). Prevalence and drug resistance characteristics of carbapenem resistant Enterobacteriaceae in Hangzhou, China. Frontiers of Medicine 12(2):182-188. Crossref