African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5176

Full Length Research Paper

Isolation of bacterial diversity present in medical waste and health care settings in hospitals in Kenya

Susan Muthoni Maina
  • Susan Muthoni Maina
  • Department of Medical Microbiology, Jomo Kenyatta University of Agriculture and Technology (JKUAT), Kenya.
  • Google Scholar
Andrew K. Nyerere
  • Andrew K. Nyerere
  • Department of Medical Microbiology, Jomo Kenyatta University of Agriculture and Technology (JKUAT), Kenya.
  • Google Scholar
Caroline Wangari Ngugi
  • Caroline Wangari Ngugi
  • Department of Medical Microbiology, Jomo Kenyatta University of Agriculture and Technology (JKUAT), Kenya.
  • Google Scholar


  •  Received: 02 May 2018
  •  Accepted: 04 June 2018
  •  Published: 14 July 2018

References

Allegranzi B, Pittet, D. (2009). Role of Hand Hygiene in Healthcare-Associated Infection Prevention. The Journal of Hospital Infection 73(4):305-315.
Crossref

 

Anitha J, Jayraaj IA (2012). Isolation and identification of bacteria from bio-hospital waste (BMW). International Journal of Pharmacy and Pharmaceutical Sciences 4(5):1-3.

 
 

Ashfaq K.M.A, Pijush S, Majharul I.M, Kant O.R, Chandra B.G (2013). Screening of antibiotic resistant gram negative bacteria and plasmid profiling of multi-drug resistant isolates present in sewage associated with health care centers. International Journal of Medical Research and Health Sciences 2(4): 923-930.
Crossref

 
 

Aziz RJ, Al-Zubaidy FS, Al-Mathkhury HJ, Resul B, Musenga J. (2014) Antibiogram of Escherichia coli isolated from different hospitals wastewater in Erbil City, Iraq. Iraqi Journal of Science 55(2):341-351.

 
 

Bakkali MEL, Hmid K, Kari KE, Zouhdi M, Mzibri MEL (2015). Characterization of bacterial strains and their resistance status in hospital environment. Journal of Tropical diseases 4:180.doi 10.4172/2329-891X.1000180.

 
 

Baron J, Finglod S (1996). Methods for identification of etiological agents of infectious diseases. In: St Louis, USA editors. Bally &Scotts diagnostic microbiology, 10th edition. Mosby: Inc pp. 327-529.

 
 

Boyce JM (2007). Environmental contamination makes an important contribution to hospital infection. Journal Hospital Infection 65(2):50-54.
Crossref

 
 

Boyce JM, Potter-Bynoe G, Chenevert C, King T (1997). Environmental contamination due to methicillin- resistant Staphylococcus aureus: Possible infection control implications. Journal of Infections Control and Hospital Epidemiology 18(9):622-627.
Crossref

 
 

Buchanan RE, Gibbons NE (1974). Bergey's manual of determinative bacteriology. 8th ed. Baltimore: William and Wilken P 1042.

 
 

Carvalho KS, Melo MC, Melo GB, Gontijo-Filho PP (2007). Hospital surface contamination in wards occupied by patients infected with MRSA or MSSA in a Brazilian university hospital. Journal of Basic and Applied Pharmaceutical Science 28(2):159-163.

 
 

Dudley DJ, Guentzel NM, Ibarra MJ, Moore BE, Sagik BP (1980). Enumeration of potentially pathogenic bacteria from sewage sludges. Applied Environmental Microbiology pp. 118-126.

 
 

Ekhaise FO, Omavwoya BP (2008). Influence of hospital wastewater discharged from university of Benin teaching hospital (UBTH), Benin City on its receiving environment. American-Eurasian Journal of Agricultural Environmental Science 4(4):484-488.

 
 

Ekrami A, Kayedani A, Jahangir M, Kalantar E, Jalali M (2011). Isolation of common aerobic bacterial pathogens from the environment of seven hospitals. Journal of Microbiology 4(2):75-82.

 
 

Frost M, Sullivan K (2010). Hospital acquired infections – Trends across Europe M56E-54.

 
 

Gastmeier P, Schwab F, Barwolff S, Ruden H, Grundmann H.(2006). Correlation between the genetic diversity of nosocomial pathogens and their survival time in intensive care units. Journal of Clinical Microbiology 35:1394-1397.
Crossref

 
 

Giske CG, Monnet DL, Cars O, Carmelli Y (2008). Clinical and economic impact of common multidrug- resistant Gram negative bacilli. Antimicrobial. Agents Chemotherapy 52:813-821.
Crossref

 
 

Hirai Y (1991). Survival of bacteria under dry conditions; from a viewpoint of nosocomial infection. Journal of Hospital Infections 19:191-200.
Crossref

 
 

Hospital records KNH and KMH (2014-2016).

 
 

Husein JR, Villar RN, Gray AJ, Farrington M (2001). Use of light handles in the laminar flow operating theatre-is it a cause of bacterial concern. Annals of the Royal College of Surgeons of England 83:353-354.

 
 

Kim TE, Jeong YW, Cho SH, Kim SJ, Kwon HJ (2007). Chronological study of antibiotic resistances and their relevant genes in Korean avian pathogenic E. coli isolates Journal of Clinical Microbiology 45(10):3309-3315.
Crossref

 
 

Kirby WM, Bauer AM, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by a standard simple disk method. Journal Clinical Pathology 45:493-496.
Crossref

 
 

Liberto, M.C, Matera R, Piccis, T, Lo Russo E, Colosima, E. Foca (2009), Six cases of sepsis caused by Pantoea agglomelans in a teaching hospital. New Microbial 32:119-123.

 
 

Medubi SA, Akande TM, Osagbemi GK. (2006). Awareness and pattern of needle stick injuries among health workers at university of Ilorin teaching hospital, Ilorin, Nigeria. African Journal of Clinical and Experimental Microbiology 7(3):183-187.
Crossref

 
 

Młynarczyk A1, Młynarczyk G, Pupek J, Bilewska A, Kawecki D, Łuczak M, Gozdowska J, Durlik M, Paczek L, Chmura A, Rowińnski W (2007). Serratia marscens isolated in 2005 from clinical specimens from patients with diminished immunity. Transplantation Proceedings 39(9):2879-2882.
Crossref

 
 

Moges F, Mengistu E, Yeshambel B, Walelegn W (2014). Isolation and characterization of multiple drug resistance bacterial pathogens from waste water in hospital and non-hospital environments, Northwest Ethiopia. BioMed Central Research Notes 7:215.
Crossref

 
 

Murray PR, Baron EJ Jorgesen JH, Faller PM, Yolken RH (2003). Manual of Clinical Microbiology, 8th edition. Washington, D.C; ASM Press.

 
 

Nagoba BS, Deshmukh SR, Husain RA (1997). Bacteriological analysis of various environmental sources in a rural hospital. Indian Journal Medical Sciences 51:465-469.

 
 

Nworie A, Ayeni JA, Eze UA, Azi SO (2012). Bacterial contamination of door handles/knobs in selected public conveniences in Abuja Metropolis, Nigeria: A public health threat. Continental Journal of Medical Research 6(1):7-11.

 
 

Olalekan AW, Asekun-Olarinmoye EO, Bamidele, JO, Abodunrin, OL, Olowu A (2011). A comparative study of awareness and attitude to nosocomial infections among levels of health care workers in Southwestern Nigeria. Continental Journal of Tropical Medicine 5(2):5-10.

 
 

Omoni VT, Makinde OA, Abutu SO (2015). Prevalence of multidrug resistant bacteria isolatedfrom biomedical waste generatedin Markudi Metropolis, Benue State, Nigeria. British Microbiology Research Journal 10(3): Article no. BMRJ.17384 

View
Crossref

 
 

Onweremadu EU, Chukwuma MC, Ogbonna FC (2009). Spatial Variability of Mercury in Soils Affected by Automobile Wastes at Port-harcourt, Nigeria. Advances in Natural and Applied Sciences 3(2):260-264.

 
 

Oyeleke SB, Istafanus N (2009). The microbiological effects of hospital wastes on the environment. Journal of Biotechnology 8(220):6253-6257.
Crossref

 
 

Oyiasogie FE, Ajuzie CU, Ighodaro UG (2010). Bacterial analysis of soil from waste dumpsite. Archives of Applied Science Research 2(5):161-167.

 
 

Pal RB, Rodrigues M, Datta S (2010). Role of Pseudomonas in nosocomial infections and biological characterization of local strains. Journal of Bioscience Technology 1(4):170-179.

 
 

Perwaiz S, Barakzi Q, Farooqi BJ, Khursheed N, Sabir N (2007). Antimicrobial susceptibility pattern of clinical isolates of methicillin resistant Staphylococcus aureus. Journal of Pakistan Medical Association 57: 2-7

 
 

Rajat R, Ninama G, Mistry K, Parmar R, Patel K, Vegad, M (2012). Antibiotic resistance pattern in Pseudomonas species isolated at a tertiary care hospital, Ahmadabad, National Journal of Medicine 2(2):156-159.

 
 

Rupp ME, Fitzgerald T, Puumala S, Anderson JR, Craig R (2008). Prospective, controlled, crossover trial of alcohol-based hand gel in critical care units. Infection Control Hospital Epidemiology 29:8-15.
Crossref

 
 

Rutala WA, Weber DJ (2014). Selection of the ideal disinfectant infect control Hospital Epidemiology 35:855-865.

 
 

Sridhar MK, Olajumoke B, Ayeni J (2003). Infection potential of wastes from selected healthcare facilities In Ibadan, Nigeria, in Martin J. Bunch, V. Madha Suresh and T. Vasantha Kumaran, eds., Proceedings of the Third International Conference on Environment and Health, Chennai, India, 15-17 December, 2003. Chennai: Department of Geography, University of Madras and Faculty of Environmental Studies, York University pp. 512-519.

 
 

Srinivas B, Aruna SB, Nayak SK, Rao, BN (2015). A study of drug resistance of methicillin resistance Staphylococcus aureus (MRSA) in rural and urban tertiary care centers. World Journal of Pharmacy and Pharmaceuticals Sciences 4(06):1144-1155.

 
 

Toralo, P.K (2005) Foundation in Microbiology, Bacteriology. 5th edition, The McGraw Hill Companies, New York.

 
 

Thurston J, Cysewsk ST (1979). The pathogenic fungi and the pathogenic actinomycetis in J.W Rippon (editon), Medical Mycology pp. 697-699.

 
 

Udeze AO, Adeyemi AT, Adeniji FO, Nwanze JC, Onoh C, Okerentubga, P.O. Okonko I.O. (2012). Plasmid mediated ampicillin resistant bacteria isolates from University of Ilorin Health Centre. New York Science Journal 5(4):56-63.

 
 

Vichal R, Pooja R, Shalini B (2011). Bacteriological profile of bio-hospital waste: Management guidelines. Journal of Indian Academy of Forensic Medicine 33(2):145-147.

 
 

Wendt C, Dietze B, Dietz E, Ruden H (1997). Survival of Acinetobacter baumanni on dry surfaces. Journal of Clinical Microbiology 35:1394-1397.

 
 

World Health Organization (2002). Antibiotic resistance. WHO Media center WHO/Geneva.

View

 
 

www.knh.or.ke/wikipedia/about Kenyatta National Hospital (KNH) retrieved on 23rd October 2016 at 03.20 GMT. October Issue (3)2014 pp. 1-16 at 01.30 GMT.

 
 

Yagoub SO, Amani EL Agbah (2010). Isolation of potential pathogenic bacteria from the air of hospital. Journal of Applied Science 10:1011- 1014.
Crossref

 
 

Yismaw G, Abay S, Asrat D, Yifru S, Kassu A (2010). Bacteriological profiles and drug resistance pattern of clinical isolates from pediatric patients, Gondar University Teaching Hospital, Gondar Northwest Ethiopia. Ethiopian Medical Journal 18:293-300.