African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4962

Full Length Research Paper

Purification and characterisation of a novel broad spectrum anti-tumor L-glutaminase enzyme from marine Bacillus subtilis strain JK-79

Jambulingam Kiruthika
  • Jambulingam Kiruthika
  • Department of Industrial Biotechnology, Government College of Technology, Coimbatore, Tamil Nadu, India.
  • Google Scholar
Sudhakar Swathi
  • Sudhakar Swathi
  • Department of Industrial Biotechnology, Government College of Technology, Coimbatore, Tamil Nadu, India.
  • Google Scholar


  •  Received: 03 July 2017
  •  Accepted: 22 March 2019
  •  Published: 28 March 2019

References

Botre F, Botre C, Lorenti G, Mazzei F, Porcelli F, Scibona G (1993). Determination of L-glutamate and L-glutamine in pharmaceutical formulation by amperometric L-glutamate oxidase-based enzyme sensors. Journal of Pharmaceutical and Biomedical Analysis 11:679-686.
Crossref

 

Brosnan JT, Ewart HS, Squires SA (1995). Hormonal control of hepatic glutaminase, Advances in Enzyme Regulation 35:131-146.
Crossref

 
 

El-Ghonemy HD (2014). Microbial amidases and their industrial applications: A review. Journal of Medical Microbilogy and Diagnosis 4(173):1-6.

 
 

Holcenberg JS (1982). Enzyme Therapy: Problems and Solutions. Annual review of Biochemistry 51:795-812.
Crossref

 
 

Imada A, Igarasi S, Nakahama K, Isono M (1973). Asparaginase and glutaminase activities of microorganisms. Journal of General Microbiology 76:85-99.
Crossref

 
 

Iwasa T, Fujii M, Yokotsuka T (1987). Glutaminase produced by Cryptococcus albidus ATCC20293. Purification and some properties of the enzyme. Nippon Shoyu Kenkyusho Zasshi 13:205-210.

 
 

Iyer P, Singhal RS (2009). Screening and selection of marine isolate for L-glutaminase production and media optimization using response surface methodology. Applied Biochemistry and Biotechnology 159:233-250.
Crossref

 
 

Jeon J, Lee HI, Han SH, Chang CS, So JS (2010). Partial Purification and Characterisation of Glutaminase from Lactobacillus reuteri KCTC3594. Applied Biochemistry and Biotechnology 162:146-154.
Crossref

 
 

Kiruthika J, Saraswathy N (2014). Isolation and characterization of a novel L-glutaminase producing marine Bacillus subtilis JK-79. Asian Journal of Microbiology Biotechnology and Environmental Science 16(3):601-610.

 
 

Klien M, Kaltwassar H, Jahns T (2002). Isolation of a novel, phosphate-activated glutaminase from Bacillus pasteurii. FEMS Microbiology Letters 206:63-67.
Crossref

 
 

Laemmli UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
Crossref

 
 

Levintow L (1954). The glutamyltransferase activity of normal and neoplastic tissues. Journal of National Cancer Institute 15:347-352.

 
 

Lineweaver-Burk DH (1932). The determination of enzyme diassociation constants. Journal of the American Chemical Society 56:658-666.
Crossref

 
 

Lokendra K, Balvinder S, Dilip KA, Joydeep M, Debashish G (2012). A Temperature and salt- tolerant L-glutaminase from Gangotri Region of Uttarakhand Himalaya: Enzyme Purification and Characterization. Applied Biochemistry and Biotechnology 166:1723-1735.
Crossref

 
 

Lowry OH, Rosebrough, NN, Farr AL, Randall RY (1951). Protein measurement with the Folin Phenol reagent. The Journal of Biological Chemistry 193:265-275.

 
 

Moriguchi M, Sakai K, Tateyama R, Furuta Y, Wakayama M (1994). Isolation and characterization of salt-tolerant glutaminases from marine Micrococcus luteus K-3. Journal of fermentation and Bioengineering 77:621-625.
Crossref

 
 

Mosmann T (1983). Rapid colorimetric assay for cellular growth and survival: application for proliferation and cytotoxity assay. Journal of Immunological Methods 65:55-63.
Crossref

 
 

Mulchandani A, Bassi AS (1996). Determination of glutamine and glutamic acid in mammalian cell cultures using tetrathiafulvalene modified enzyme electrodes. Biosensor Bioelectronics 11:271-280.
Crossref

 
 

Nakadai T, Nasuno S (1989). Use of glutaminase for soy sauce made by Koji or a preparation of proteases from Aspergillus oryzae. Journal of Fermentation and Bioengineering 67:158-162.
Crossref

 
 

Nandakumar R, Yoshimune K, Wakayama M, Moriguchi M (2003). Microbial glutaminase: biochemistry, molecular approaches and applications in the food industry. Journal of Molecular Catalysis: B Enzymatic 23:87-100.
Crossref

 
 

Narta UK, Kanwar SS, Azmi W (2007). Pharmacological and clinical evaluation of L-asparginase in the treatment of Leukemia. Critical Reviews in Oncology/Hematology 61:208-221.
Crossref

 
 

Gurung N, Ray S, Bose S, Rai V (2013). A Broader View: Microbial Enzymes and Their Relevance in Industries, Medicine, and Beyond. BioMed Research International 18 p.
Crossref

 
 

Noura E-A, El-Naggar E, El-Ewasy S, Nancy M, El-Shweihy M (2014). Microbial L-asparginase as a potential therapeutic agent for the treatment of Acute Lymphoblastic Leukemia: The Pros and Cons. International Journal of Pharmacology 10(4):182-199.
Crossref

 
 

Peng C, Chen S, Lin F, Lin Z (2000). Detection of anti-oxidative capacity in plants by scavenging organic free radical DPPH. Progress in Biochemistry and Biophysics 27(6):57-61.

 
 

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999). Anti-oxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine 26:1231-1237.
Crossref

 
 

Riberg B, Torgner IA, Kvamme E (1995). The orientation of phosphate activated glutaminase in the inner mitochondrial membrane of synaptic and non-synaptic rat brain mitochondria. Neurochemistry International 27:367-376.
Crossref

 
 

Roberts J, Mac Allister TW, Sethuraman N, Freeman AG (2001). Genetically engineered glutaminase and its use in anti-viral and anti-cancer therapy. US Patent 6312939.

 
 

Sabu A (2003). Sources, properties and applications of microbial therapeutic enzymes. Indian Journal of Biotechnology 2:334-335.

 
 

Sabu A, Chandrasekaran M, Pandey A (2000b). Biopotential of microbial glutaminases. Chemistry Today 18:21-25.

 
 

Sabu A, Keerthi TR, Kumar SR, Chandrasekaran M (2000a). L-glutaminase production by marine Beauveria sp. under solid state fermentation. Process Biochemistry 35:705-710.
Crossref

 
 

Sathish T, Prakasham RS (2010). Enrichment of glutaminase production by Bacillus subtilis RSP-GLU in submerged cultivation based on neural network–genetic algorithm approach. Journal of Chemical Technology and Biotechnology 85:50-58
Crossref

 
 

Schmid FA, Roberts J (1974). Anti-neoplastic and toxic effects of Acinetobacter and Pseudomonas glutaminase-asparaginases, Cancer Chemotherapy Reports 58:829-840.

 
 

Singh P, Banik RM (2013). Biochemical Characterization and Anti-tumor Study of L- Glutaminase from Bacillus cereus MTCC 1305. Applied Biochemistry and Biotechnology 171:522-531.
Crossref

 
 

Sinsuwan S, Yongsawatdigul J, Chumseng S, Yamabhai M (2012). Efficient expression and purification of recombinant glutaminase from Bacillus licheniformis (GlsA) in Escherichia coli. Protein expression and Purification. 83:52-58.
Crossref

 
 

Tachiki T, Yamada T, Mizuno K, Ueda M, Shiode J, Fukami H (1998). gamma-Glutamyl transfer reactions by glutaminase from Pseudomonas nitroreducens IFO 12694 and their application for the syntheses of theanine and glutamylmethyl amide. Bioscience Biotechnology Biochemistry 62:1279-1283.
Crossref

 
 

Wakayama M, Yamagata T, Kamemura A, Bootim N, Yano S, Tachiki T, Yoshimune K, Moriguchi M (2005). Characterisation of salt-tolerant glutaminase from Stenotrophomonas maltophilia NYW-81 and its application in Japanese soy sauce fermentation. Journal of Industrial Microbiology and Biotechnology 32:383-390.
Crossref

 
 

World Health Statistics (2018). World Health Organization 2018 Geneva: WHO Press, 2018.

 
 

Yano T, Ito M, Tomito K, Kumagai H (1988). Purification and properties of glutaminase from Aspergillus oryzae. Journal of Fermentation Technology 66:137-143.
Crossref

 
 

Yogendra S, Ravi KG, Medicherla VJ, Srivastava SK (2013). Extracellular L-asparginase from a protease-deficient Bacillus aryabhattai ITBHU02: Purification, biochemical charcterisation and evaluation of anti-neoplastic activity in vitro. Applied Biochemistry and Biotechnology 171:1759-1774.
Crossref

 
 

Zhao J, Lopez AL, Erichsen D, Herek S, Cotter RL, Curthoys NP, Zheng J (2004). Mitochondrial glutaminase enhances extracellular glutamate production in HIV-1-infected macrophages: Linkage to HIV-1 associated dementia. Journal of Neurochemistry 88:169-180.
Crossref