African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5254

Full Length Research Paper

Colorimetric gold nanoparticles-based assay for direct detection of Clostridium difficile in clinical isolates from Qatar

Tahani M. Al-Saadi
  • Tahani M. Al-Saadi
  • College of Arts and Sciences, Qatar University, Doha, Qatar.
  • Google Scholar
Nahla O. Eltai*
  • Nahla O. Eltai*
  • Biomedical Research Center, Qatar University, Doha, Qatar.
  • Google Scholar
Wedad S. Hamdi
  • Wedad S. Hamdi
  • Biomedical Research Center, Qatar University, Doha, Qatar.
  • Google Scholar
Fadheela Dad Bakhsh
  • Fadheela Dad Bakhsh
  • Biomedical Research Center, Qatar University, Doha, Qatar.
  • Google Scholar
Hassan Azzazy
  • Hassan Azzazy
  • Department of Chemistry, American University of Cairo, Egypt.
  • Google Scholar
Nasser Al-Ansari
  • Nasser Al-Ansari
  • Hamad Medical Corporation, Al-Khor Hospital, Doha, Qatar.
  • Google Scholar
Asmaa Al-Thani
  • Asmaa Al-Thani
  • College of Arts and Sciences, Qatar University, Doha, Qatar.
  • Google Scholar


  •  Received: 09 March 2017
  •  Accepted: 10 May 2017
  •  Published: 21 May 2017

References

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Burnham CAD, Carroll KC (2013). Diagnosis of clostridium difficile infection: An ongoing conundrum for clinicians and for clinical laboratories. Clin. Microbiol. Rev. 26(3):604-630.
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Hussain MM, Samir, TM, Azzazy HME (2013). Unmodified gold nanoparticles for direct and rapid detection of Mycobacterium tuberculosis complex. Clin. Biochem. 46(7-8):633-637.
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Jennings T, Strouse G (2007). Past, present, and future of gold nanoparticles. Adv. Exp. Med. Biol. 620:34-47.
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Persson S, Jensen JN., Olsen KEP (2011). Multiplex PCR method for detection of Clostridium difficile tcdA, tcdB, cdtA, and cdtB and internal in-frame deletion of tcdC. J. Clin. Microbiol. 49(12):4299-300.
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Shawky SM, Bald D, Azzazy HME (2010). Direct detection of unamplified hepatitis C virus RNA using unmodified gold nanoparticles. Clin. Biochem. 43:1163-1168.
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Syed MA (2014). Advances in nanodiagnostic techniques for microbial agents. Biosen. Bioelectron. 51:391-400.
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Ticehurst JR, Aird DZ, Dam LM, Borek AP, Hargrove JT, Carroll KC (2006). Effective detection of toxigenic Clostridium difficile by a two-step algorithm including tests for antigen and cytotoxin. J. Clin. Microbiol. 44:1145-1149.
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West JL, Halas NJ (2003). Engineered nanomaterials for biophotonics applications: improving sensing, imaging, and therapeutics. Annu. Rev. Biomed. Eng. 5:285-292.
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Zhu Z, Shi L, Feng H, Zhou HS (2015). Single domain antibody coated gold nanoparticles as enhancer for Colstridium difficile toxin detection by electrochemical impedance immunosensors. Bioelectrochemistry 101:153-158.
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