African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5254

Full Length Research Paper

Development and application of an enzyme-linked immunosorbent assay (ELISA) using a soluble recombinant nucleoprotein for the detection of antibodies to avian influenza virus

Mariana Monezi Borzi*
  • Mariana Monezi Borzi*
  • Laboratory of Immunology and Virology, Department of Veterinary Pathology, São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal, São Paulo, Brazil.
  • Google Scholar
Ketherson Rodrigues Silva
  • Ketherson Rodrigues Silva
  • Laboratory of Immunology and Virology, Department of Veterinary Pathology, São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal, São Paulo, Brazil.
  • Google Scholar
Maria de Fatima Silva Montassier
  • Maria de Fatima Silva Montassier
  • Laboratory of Immunology and Virology, Department of Veterinary Pathology, São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal, São Paulo, Brazil.
  • Google Scholar
Filipe Santos Fernando
  • Filipe Santos Fernando
  • Laboratory of Immunology and Virology, Department of Veterinary Pathology, São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal, São Paulo, Brazil.
  • Google Scholar
Maria de Lourdes Feres Tamanine
  • Maria de Lourdes Feres Tamanine
  • Laboratory of Immunology and Virology, Department of Veterinary Pathology, São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal, São Paulo, Brazil.
  • Google Scholar
Romeu Moreira dos Santos
  • Romeu Moreira dos Santos
  • Laboratory of Immunology and Virology, Department of Veterinary Pathology, São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal, São Paulo, Brazil.
  • Google Scholar
Elisabete Schirato de Oliveira
  • Elisabete Schirato de Oliveira
  • Laboratory of Immunology and Virology, Department of Veterinary Pathology, São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal, São Paulo, Brazil.
  • Google Scholar
Viviane Casagrande Mariguela
  • Viviane Casagrande Mariguela
  • Laboratory of Immunology and Virology, Department of Veterinary Pathology, São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal, São Paulo, Brazil.
  • Google Scholar
Priscila Diniz Lopes
  • Priscila Diniz Lopes
  • Laboratory of Immunology and Virology, Department of Veterinary Pathology, São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal, São Paulo, Brazil.
  • Google Scholar
Dilmara Reischak
  • Dilmara Reischak
  • National Agricultural Laboratory (LANAGRO), Campinas, São Paulo, Brazil.
  • Google Scholar
Andre Oliveira Mendonca
  • Andre Oliveira Mendonca
  • National Agricultural Laboratory (LANAGRO), Campinas, São Paulo, Brazil.
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Renato Luis Luciano
  • Renato Luis Luciano
  • Advanced Center for Technological Research of Poultry Agribusiness /Biological Institute, Descalvado, São Paulo, Brazil.
  • Google Scholar
Helio Jose Montassier
  • Helio Jose Montassier
  • Laboratory of Immunology and Virology, Department of Veterinary Pathology, São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal, São Paulo, Brazil.
  • Google Scholar


  •  Received: 06 December 2016
  •  Accepted: 23 March 2017
  •  Published: 14 May 2017

References

Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein of protein-dye binding. Anal. Biochem. 72(1-2):248-254.
Crossref

 

Deng G, Tan D, Shi J, Cui P, Jiang Y, Liu L, Tian G, Kawaoka Y, Li C, Chen H (2013). Complex Reassortment of Multiple Subtypes of Avian Influenza Viruses in Domestic Ducks at the Dongting Lake Region of China. J. Virol. 87(17):9452-9462.
Crossref

 

Gibertoni AM, Montassier MF, Sena JA, Givisiez PE, Furuyama CR, Montassier HJ (2005). Development and application of a Saccharomyces cerevisiae-expressed nucleocapsid protein-based enzyme-linked immunosorbent assay for detection of antibodies against Infectious Bronchitis virus. J. Clin. Microbiol. 43(4):1982-1984.
Crossref

 

Gopal GJ, Kumar A (2013). Strategies for the Production of Recombinant Protein in Escherichia coli. Protein J. 32:419-425
Crossref

 

Guerrero F, Ciragan A, Iwaï H (2015). Tandem SUMO fusion vectors for improving soluble protein expression and purification. Protein Expr. Purif.116:42-49.
Crossref

 

Hanson BA, Stallknecht DE, Swayne DE, Lewis LA, Senne DA (2003). Avian Influenza Viruses in Minnesota Ducks During 1998-2000. Avian Dis. 47(s3):867-871.
Crossref

 

Henriques AM, Fagulha T, Barros SC, Ramos F, Duarte M, Luís T, Fevereiro M (2011). Multiyear surveillance of influenza A virus in wild birds in Portugal. Avian Pathol. 40(6):597-602.
Crossref

 

Hinshaw VS, Webster RG, Rodriguez RJ (1981). Influenza A viruses: Combinations of hemagglutinin and neuraminidase subtypes isolated from animals and other sources. Arch. Virol. 67(3):191-201.
Crossref

 

Hopp TP, Woods KR (1981). Prediction protein antigenic determinants from amino acid sequences. Proc. Natl. Acad. Sci. USA. 78(6):3824-3828.
Crossref

 

Jensen TH, Ajjouri G, Handberg KJ, Slomka MJ, Coward VJ, Cherbonnel M, Jestin V, Lind P, Jogensen PH (2013). An enzyme-linked immunosorbent assay for detection of Avian Influenza Virus subtypes H5 and H7 antibodies. Acta Vet. Scand. 55(1):84.
Crossref

 

Jin M, Wang G, Zhang R, Zhao S, Li H, Tan Y, Chen H (2004). Development of enzyme-linked immunosorbent assay with nucleoprotein as antigen for detection of antibodies to Avian Influenza Virus. Avian. Dis. 48(4):870-878.
Crossref

 

Kang HM, Choi JG, Kim KI, Park HY, Parkt CK, Lee YJ (2013). Genetic and antigenic characteristics of H4 subtype avian influenza viruses in Korea and their pathogenicity in quails, domestic ducks and mice. J. Gen. Virol. 94(Pt 1):30-39.
Crossref

 

Karasin AI, Brown IH, Carman S, Olsen CW (2000). Isolation and Characterization of H4N6 Avian Influenza Viruses from Pigs with Pneumonia in Canada. J. Virol. 4(19):9322-9327.
Crossref

 

Koppel Z, Vrtiak J,Vasil M, Spiesz St (1956). Mass illness of ducklings in Eastern Slovakia with clinical picture of infectious sinusitis. Veterinarstvi 6:267-268.

 

Landis JR, Koch GG (1977). An application of hierarchical kappa-type statistics in the assessment of majority agreement among multiple observers. Biometrics 33(2):363-374.
Crossref

 

Larsen JE, Lund O, Nielsen M (2006). Improved method for predicting linear B-cell epitopes. Immunome Res. 2:2.
Crossref

 

Latorre-Margalef N, Tolf C, Grosbois V, Avril A, Bengtsson D, Wille M, Osterhaus ADME, Fouchier RAM, Olsen B, Waldeström J (2014). Long-term variation in influenza A virus prevalence and subtype diversity in migratory mallards in northern Europe. Proc. R. Soc. B 281:20140098.
Crossref

 

Liu M, He S, Walker D, Zhou N, Perez DR, Mo B, Li F, Huang X, Webster RG, Webby R (2003). The influenza virus gene pool in a poultry market in South Central China. Virology 305:267-275
Crossref

 

Mahmood T, Yang PC (2012). Western blot: technique, theory, and trouble shooting. N. Am. J. Med. Sci. 4(9):429-434.
Crossref

 

Mohan CM, Dey S, Rai A, Kataria JM (2006). Recombinant haemagglutinin-neuraminidase antigen-based single serum dilution ELISA for rapid serological profiling of Newcastle disease virus. J. Virol. Methods 138(1-2):117-122.
Crossref

 

Okamatsu M, Nishi T, Nomura N, Yamamoto N, Sakoda Y, Sakurai K, Chu HD, Thanh LP, Nguyen LV, Hoang NV, Tien TN, Yoshida R, Takada A, Kida H (2013). The genetic and antigenic diversity of avian influenza viruses isolated from domestic ducks, muscovy ducks, and chickens in northern and southern Vietnam, 2010–2012. Virus Genes 47:317-329.
Crossref

 

Olsen B, Munster VJ, Wallensten A, Waldenstrom J, Osterhaus AD, Fouchier RA (2006). Global patterns of Influenza A virus in wild birds. Science 312(5772):384-388.
Crossref

 

Rosano GL, Ceccarelli EA (2014). Recombinant protein expression in Escherichia coli: advances and challenges. Front. Microbiol. 5:172
Crossref

 

Sambrook J, Russel DW (2001). Molecular Cloning: a laboratory manual (3Volume Set), 3ed. Cold Spring Harbor Laboratory Press, 1531p.

 

Scotch M, Lam TTY, Pabilonia KL, Anderson T, Baroch J, Kohler D, DeLiberto TJ (2014). Diffusion of influenza viruses among migratory birds with a focus on the Southwest United States. Infect. Genet. Evol. 26:185-193.
Crossref

 

Shafer AL, Katz JB, Ernisse KA (1998). Development and validation of a competitive enzyme-linked immunosorbent assay for detection of type A Influenza antibodies in avian sera. Avian Dis. 42(1):28-34.
Crossref

 

Shi Y, Cui H, Wang J, Chi Q, Li X, Teng Q, Chen H, Yang J, Liu Q, Li Z (2016). Characterizations of H4 avian influenza viruses isolated from ducks in live poultry markets and farm in Shanghai. Sci. Rep. 6:37843.
Crossref

 

Silva KR, Gonçalves MCM, Oliveira ES, Fernando FS, Montassier MFS, Fernandes CC, Tamanine MLF, Borzi MM, Santos RM, Mendonça AO, Reischack D, Paulillo AC, Montassier HJ (2014). Cloning and expression of the nucleoprotein gene (NP) of Newcastle Disease Virus (NDV) in Escherichia coli for Immunodiagnosis application. Int. J. Poult. Sci. 13(8):473-479.
Crossref

 

Swayne DE, Halvorson DA (2003). Influenza, In. Saif YM (Ed.), Diseases of Poultry, 11th Ed. Iowa State Press, Ames, Iowa. Pp. 135-160.

 

Upadhyay C, Ammayappan A, Vakharia VN (2009). Detection of NP, N3 and N7 antibodies to Avian Influenza Virus by indirect ELISA using yeast expressed antigens. J. Virol. 6:158-167.
Crossref

 

Varich NL, Kaverin NV (2004). Antigenically relevant amino acid positions as revealed in reactions of monoclonal antibodies with the nucleoproteins of closely related Influenza A Virus strains. Arch. Virol. 149(11):2271-2276.
Crossref

 

Wilcox BR, Knutsen GA, Berdeen J, Goekjian V, Poulson R, Goyal S, Sreevatsan S, Cardona C, Berghaus RD, Swayne DE, Yabsley MJ, Stallknecht DE (2011). Influenza-A Viruses in Ducks in Northwestern Minnesota: Fine Scale Spatial and Temporal Variation in Prevalence and Subtype Diversity. PLoS One 6(9):e24010.

 

Wu R, Hu S, Xiao Y, Li Z, Shi D, Bi D (2007). Development of indirect enzyme-linked immunosorbent assay with nucleoprotein as antigen for detection and quantification of antibodies against Avian Influenza Virus. Vet. Res. Commun. 31(5):631-641.
Crossref

 

Yang M, Berhane Y, Salo T, Li M, Hole K, Clavijo A (2008). Development and application of monoclonal antibodies against Avian Influenza Virus nucleoprotein. J. Virol. Methods 147(2):265-274.
Crossref

 

Zuo X, Mattern MR, Tan R, Li S, Hall J, Sterner DE, Shoo J, Tran H, Lim P, Sarafianos SG, Kazi L, Navas-Martin S, Weiss SR, Butt TR (2005). Expression and purification of SARS coronavirus proteins using SUMO-fusions. Protein. Expr. Purif. 42(1):100-110.
Crossref