African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5215

Full Length Research Paper

Cholesterol reduction in vitro by novel probiotic lactic acid bacterial strains of Enterococcus isolated from healthy infants’ stool

Akram A. Aboseidah
  • Akram A. Aboseidah
  • Department of Botany and Microbiology, Faculty of Science, Suez University, Suez, Egypt.
  • Google Scholar
Abdel-Hamied M. Rasmey
  • Abdel-Hamied M. Rasmey
  • Department of Botany and Microbiology, Faculty of Science, Suez University, Suez, Egypt.
  • Google Scholar
Magdy M. Osman
  • Magdy M. Osman
  • Department of Dairy, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt.
  • Google Scholar
Salha G. Desouky
  • Salha G. Desouky
  • Department of Botany and Microbiology, Faculty of Science, Suez University, Suez, Egypt.
  • Google Scholar
Nehal Kamal
  • Nehal Kamal
  • Department of Botany and Microbiology, Faculty of Science, Suez University, Suez, Egypt.
  • Google Scholar


  •  Received: 04 May 2017
  •  Accepted: 07 August 2017
  •  Published: 14 October 2017

References

Aboseidah AA, Rasmey AM, Osman MM, Kamal N, Desouky SG (2017). Optimization of lactic acid production by a novel strain, Enterococcus faecalis KY072975 isolated from infants stool in Egypt. Eur. J. Biol. Res. 7:22-30.

 

Ahn YT, Kim GB, Lim KS, Baek Y J, Kim HU (2003). Deconjugation of bile salts by Lactobacillus acidophilus isolates. Int. Dairy J. 13:303-311.
Crossref

 
 

Annuk H, Shchepetova J, Kullisaar T, Songisepp E, Zilmer M, Mikelsaar M (2003). Characterization of intestinal lactobacilli as putative probiotic candidates. J. Appl. Microbiol. 94:403-412.
Crossref

 
 

Axelsson L (1998). Lactic Acid Bacteria: Classification and Physiology, In: S. Salminen, Von Wright, A., Ouwehand, A. (Ed.), Lactic Acid Bacteria:Microbiological and Functional Aspects. New York: Marcel Dekker Inc. pp. 1-72.

 
 

Casas IA, Dobrogosz WJ (2000). Validation of the Probiotic Concept: Lactobacillus reuteri confers broad-spectrum protection against disease in humans and animals. Microb. Ecol. Health. Dis. 12:247-285.
Crossref

 
 

Collins CH, Lyne PM (1985). Microbiological methods. 5th edn. Butterworths, London. https://books.google.com.eg/books?isbn=0121361101.

 
 

Davidson MH, Dillon MA, Gordon B, Jones P, Samuels J, Weiss S, Isaacsohn J, Toth P, Burke SK (1999). Colesevelam hydrochloride (Cholestagel): a new, potent bile acid sequestrant associated with a low incidence of gastrointestinal side effects. Arch. Int. Med. 159:1893-1900.
Crossref

 
 

De Man J, Rogosa M, Sharpe ME (1960). A medium for the cultivation of lactobacilli. J. Appl. Bacteriol. 23:130-135.
Crossref

 
 

Doukyu N (2009). Characteristics and biotechnological applications of microbial cholesterol oxidase. Appl. Microbiol. Biotechnol. 83:825-837.
Crossref

 
 

Dunn-Emke S, Weidner G, Ornish D (2001). Benefits of a low fat plant-based diet. Obesity 9:731.
Crossref

 
 

Ezendam J, Van Loveren H (2006). Probiotics: immunomodulation and evaluation of safety and efficacy. Nutr. Rev. 64:1-14.
Crossref

 
 

Gill HS (2003). Probiotics to enhance anti-infective defences in the gastrointestinal tract. Best Pract. Res. Clin. Gastroenterol. 17(5):755-773.
CrossrefX

 
 

Gilliland SE, Walker DK (1990). Factors to consider when selecting a culture of Lactobacillus acidophilus as a dietary adjunct to produce a hypocholesterolemic effect in humans. J. Dairy Sci. 73:905-911.
Crossref

 
 

Guo L, Yang L, Huo G (2011). Cholesterol removal by Lactobacillus plantarum isolated from homemade fermented cream in inner Mongolia of China. Czech J. Food. Sci. 29:219-225.

 
 

Guo L, Li T, Tang Y, Yang L, Huo G (2016). Probiotic properties of Enterococcus strains isolated from traditional naturally fermented cream in China. Microb. Biotechnol. 9: 737-745.
Crossref

 
 

Hu X, Wang T, Li W, Jin F, Wang L (2013). Effects of NS Lactobacillus strains on lipid metabolism of rats fed a high-cholesterol diet. Lipids Health Dis. 12:67.
Crossref

 
 

Huang Y, Wang X, Wang J, Wu F, Sui Y, Yang L, Wang Z (2013). Lactobacillus plantarum strains as potential probiotic cultures with cholesterol-lowering activity. J. Dairy Sci. 96:2746-2753.
Crossref

 
 

Kimoto H, Ohmomo S, Okamoto T (2002). Cholesterol removal from media by lactococci. J. Dairy Sci. 85:3182-3188.
Crossref

 
 

Kovalenko NK, Kasumova SA, Muchnik FV (2004). Screening of the strains of lactic acid bacteria possessing hypocholesterinemic activity and their practical use. J. Mikrobiol. 66:33-42.

 
 

Kozaki M, Uchimura T, Okada S (1992). Experimental manual for lactic acid bacteria. Asakurasyoten, Tokyo, Japan pp. 29-72.

 
 

Kumar A, Kumar M, Ghosh M, Ganguli A (2013). Modeling in vitro cholesterol reduction in relation to growth of probiotic Lactobacillus casei. Microbiol. Immunol. 57:100-110.
Crossref

 
 

Lekha PK, Lonsane BK (1997). Production and application of tannin acyl hydrolase: State of the art. Adv. Appl. Microbiol. 44:215-260.
Crossref

 
 

Liu ZQ, Shan HY (2006). Cholesterol, not polyunsaturated fatty acids, is target molecule in oxidation induced by reactive oxygen species in membrane of human erythrocytes. J. Cell. Biochem. Biophys. 45:185-193.
Crossref

 
 

Lye HS, Rusul G, Liong MT (2010). Removal of cholesterol by lactobacilli via incorporation and conversion to coprostanol. J. Dairy Sci. 93:1383-1392.
Crossref

 
 

Maassen CB, van-Holten-Neelen C, Balk F, den Bak-Glashouwer MJ, Leer RJ, Laman JD, Boersma WJ, Claassen E (2000). Strain-dependent induction of cytokine profiles in the gut by orally administered Lactobacillus strains. Vaccine 18:2613-2623.
Crossref

 
 

MacLachlan J, Wotherspoon ATL, Ansell RO, Brooks CJW (2000). Cholesterol oxidase: sources, physical properties and analytical applications. J. Steroid Biochem. Mol. Biol. 72:169-195.
Crossref

 
 

Motteran L, Pilone MS, Molla G, Ghisla S, Pollegioni L (2001). Cholesterol oxidase from Brevibacterium sterolicum the Relationship between covalent flavinylation and redox properties. J. Biol. Chem. 276:18024-18030.
Crossref

 
 

Naidu AS, Bidlack WR, Clemens RA (1999). Probiotic spectra of lactic acid bacteria (LAB). Crit. Rev. Food. Sci. Nutr. 39:13-126.
Crossref

 
 

Ngongang EFT, Tiencheu B, Achidi AU, Fossi BT, Shiynyuy DM, Womeni HM, François ZN (2016). Effects of Probiotic Bacteria from Yogurt on Enzyme and Serum Cholesterol Levels of Experimentally Induced Hyperlipidemic Wistar Albino Rats. Am. J. Biol. Life Sci. 4:48-55.

 
 

Niv E, Naftali T, Hallak R, Vaisman N (2005). The efficacy of Lactobacillus reuteri ATCC 55730 in the treatment of patients with irritable bowel syndrome- a double blind, placebo-controlled, randomized study. Clin. Nutr. 24:925-931.
Crossref

 
 

Ouwehand AC, Salminen SJ, Isolauri E (2002). Probiotics: an overview of beneficial effects. Antonie. van Leeuwenhoek 82:279-289.
Crossref

 
 

Ouwehand AC, Vesterlund S (2004). Antimicrobial components from lactic acid bacteria. In: S. Salminen, von Wright A, Ouwehand A. (Ed.), Lactic Acid Bacteria: Microbiological and Functional Aspects. New York: Marcel Dekker, Inc. 139:375-396.
Crossref

 
 

Pan DD, Zeng XQ, Yan YT (2011). Characterisation of Lactobacillus fermentum SM-7 isolated from koumiss, a potential probiotic bacterium with cholesterol lowering effects. J. Sci. Food. Agric. 91:512-518.
Crossref

 
 

Pereira DI, Gibson GR (2002). Effects of consumption of probiotics and prebiotics on serum lipid levels in humans. Crit. Rev. Biochem. Mol. Biol. 37:259-281.
Crossref

 
 

Pridham TG, Gottlieb D (1948). The utilization of carbon compounds by some actinomycetales as an aid for species determination. J. Bacteriol. 56:107-114.

 
 

Rigotti A, Miettinen HE, Krieger M (2003). The role of the high-density lipoprotein receptor sr-bi in the lipid metabolism of endocrine and other tissues. Endocr. Rev. 24:357-387.
Crossref

 
 

Roukas T (1993). Ethanol production from carbopods by Saccharomyces cerevisiae. Food Biotechnol. 41:296-301.

 
 

Rudel ll, Morris Md (1973). Determination of cholesterol using ophthalaldehyde. J. Lipid Res. 14:364-366.

 
 

Russell DW (2003). The enzymes, regulation and genetics of bile acids synthesis. Annu. Rev. Biochem. 72:137-174.
Crossref

 
 

Saarela M, Mogensen G, Fonden R, Ma¨tto¨ J, Mattila-Sandholm T (2000). Probiotic bacteria: safety, functional and technological properties. J. Biotechnol. 84:197-215.
Crossref

 
 

Said-AL Ahl HAH, Omer EA (2016). Essential oil content and chemical composition of eight dill (Anethum Geraveolens L) cultivars cultivated under Egyptian conditions. Int. J. Pharm. Pharm. Sci. 8:227-231.

 
 

Saranya S, Shekinah S, Rajagopal T, Vijayakumar J, Ponmanickam P (2014). Isolation and characterization of cholesterol degrading bacteria from soap and vegetable oil industrial waste. Indan. J. Biotechnol. 13:508-513.

 
 

Saxelin M, Tynkkynen S, Mattila-Sandholm T, de Vos WM (2005). Probiotic and other functional microbes: from markets to mechanisms. Curr. Opin. Biotechnol. 16:201-211.
Crossref

 
 

Shen Z, Corbin DR, Greenplate JT, Grebenok RJ, Galbraith DW, Purcell JP (1997). Studies on the mode of action of cholesterol oxidase on insect midgut membranes. Arch. Insect. Biochem. Physiol. 34:429-442.
Crossref

 
 

Sieladie DV, Zambou NF, Kaktcham PM, Cresci A, Fonteh F (2011). Probiotic properties of lactobacilli strains isolated from raw cow milk in the western highlands of Cameroon. Innov. Rom. Food. Biotechnol. 9:12-28.

 
 

Sih CJ, Tai HH, Tsong Y (1967). The Mechanism of Microbial Conversion of cholesterol into 17-Keto Steroids. J. Am. Chem. Soc. 89:1957-1958.
Crossref

 
 

Sojo MM, Bru RR, Garcia FF (2002). Rhodococcus erythropolis ATCC 25544 as a suitable source of cholesterol oxidase: cell-linked and extracellular enzyme synthesis, purification and concentration. BMC Biotechnol. 2:1-9
Crossref

 
 

Suzuki K, Bruce WR, Baptista J, Furrer R, Vaughan DJ, Krepinsky JJ (1986). Formation of cholest-4-en-3-one by intestinal bacteria and its biological effect. Cancer Lett. 33:307-316.
Crossref

 
 

Trautwein EA, Rieckhoff D, Erbersdobler HF (1998). Dietary inulin lowers plasma cholesterol and triacylglycerol and alters biliary bile acid profile in hamsters. J. Nutr. 128:1937-1943.

 
 

Tsai CC, Chou LC, Lai SE, Huang CC (2016). Effect of cholesterol lowering multiplex lactic acid bacteria on lipid metabolism in a hamster model. Afr. J. Microbiol. Res. 10(20):708-716.
Crossref

 
 

Vanderhoof JA, Young RJ (1998). Use of probiotics in childhood gastrointestinal disorders. J. Pediatr. Gastroenterol. Nutr. 27:323-332.
Crossref

 
 

Wang J, Zhang H, Chen X, Chen Y, Menghebilige Bao Q (2012). Selection of potential probiotic lactobacilli for cholesterol-lowering properties and their effect on cholesterol metabolism in rats fed a high-lipid diet. J. Dairy Sci. 95:1645-1654.
Crossref

 
 

WHO (2013). Cardiovascular Disease fact sheet No. 317, http://www.who.int/mediacentre/factsheets/fs317/en/.

 
 

Wittenberg R (1964). Hydrogen peroxide formation and catalase activity in the lactic acid bacteria. J. Gen. Microbiol. 35:13-14.
Crossref

 
 

Yazdi MT, Zahraei M, Aghaepour K, Kamranpour N (2001). Purification and partial characterization of a cholesterol oxidase from Streptomyces fradiae. Enzyme Microb. Technol. 28:410-414.
Crossref

 
 

Yehia HM, Hassanein WA, Ibraheim SM (2015). Purification and characterization of the extracellular cholesterol oxidase enzyme from Enterococcus hirae. BMC Microbiol. 15:178-190.
Crossref

 
 

Young DS (2001). Effects of diseases on clinical lab. Tests. 4th ed. Washington, D.C: AACC Press.