May 2015
Dermatophytes: Diagnosis of dermatophytosis and its treatment
The enzymatic ability of fungi to decompose keratin has long been interpreted as a key innovation in the evolution of animal dermatology. Dermatophytes are keratinophilic fungi which were originally saprophytic, but have adapted themselves to animal and human parasitism during the course of evolution. Dermatophytes are pathogens, which cause superficial mycosis. The dermatophytes have the capacity to invade keratinized...
May 2015
Alternative technique for culturing sputum for mycobacteria isolation: Feasibility, performance and effect on laboratory quality assessment - A technical note
Mycobacteria culture remains the cornerstone of tuberculosis diagnosis. Naturally contaminated samples need pre-inoculation processing but some economically challenged medical facilities may benefit from a simpler and cheaper sputum decontamination procedure. The aim of this study was to test a simple decontamination method lacking a centrifugation step to be used in conjunction with the culture on...
May 2015
Effect of different temperature levels and time intervals on germination of uredospores of Puccinia sorghi
In plant disease development, temperature is a critical factor. To determine how specific environmental variables affect corn rust, we determined temperature effects on urediniospore germination. Spore germination was maximum within 24 h of incubation, in the temperature range of 20-30°C. The mean maximum spore germination was recorded at 25°C (66.16% germination) which is statistically on par at 30°C...
May 2015
Occurrence and antibiogram profile of Staphylococcus aureus isolated from some hospital environment in Zaria, Nigeria
Staphylococcus aureus is an opportunistic pathogen and is the most frequent cause of hospital acquired infection. A total sample of 310 was collected for this work; the pathogen was isolated from hands of some healthcare workers/nurses, operation tables, door knobs/door handles, nurses’ table tops, bedrails, stretchers, floors, toilets seats, cupboards, and sinks. The total percentage prevalence of the pathogen in...
May 2015
Identification, antibiotic resistance and distribution of different classes of integrons among proteus species isolated from different sources in Dakahleia and Damietta Egyptian Governorates
The aim of the present investigation is to trace the epidemiological relatedness between different isolates of proteus sp. obtained from Dakahleia governorate as compared with similar sources from the adjacent Damietta governorate in Egypt. A total of 500 bacterial isolates were collected from human and animal sources including urine samples, sputum, wound, throat and ear swabs, endotracheal aspirate, breast swab, bile...
May 2015
Colletotrichum gloeosporioides lipase: Characterization and use in hydrolysis and esterifications
Colletotrichum gloeosporioides was evaluated for its capacity to produce extracellular lipase. A crude enzyme preparation obtained after 48 h of fermentation reached 742 U/L of lipolytic activity. Estimated molecular weight of proteins responsible for this activity was about 18 kDa as determined by SDS-PAGE and zymogram analysis. Enzyme preparation showed optimum pH at 10 and stability at optimum temperature (37°C)...
May 2015
Pseudomonas fluorescens (Pf1) mediated chitinolytic activity in tomato plants against Fusarium oxysporum f. sp. lycopersici
Liquid formulation of Pseudomonas fluorescens strain Pf1 was found to protect tomato plants from wilt disease caused by Fusarium oxysporum f. sp. lycopersici (FOL). The lytic enzyme and chitinase activity were assayed in the tomato plants treated with liquid as well as talc based formulations of Pf1 and challenged with the FOL. The chitinase activity was significantly increased in tomato plants treated with sequential...
May 2015
Seroprevalence and risk factors of small ruminant brucellosis in selected districts of Arsi and East Shoa zones, Oromia region, Ethiopia
A cross-sectional study was conducted in two districts in Arsi zone and one district in East Shoa zone, Ethiopia, to determine seroprevalence and assess the possible risk factors associated with small ruminant brucellosis. A total of 840 blood samples (409 sheep and 431goats) were collected. All sera samples were screened by modified Rose Bengal Test (mRBT) and all positive reactors were further tested by indirect...
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