January 2015
Physico-chemical surface characterization of Bacillus cereus spores isolated from an Algerian dairy plant
Bacillus cereus is an endospore-forming bacterium frequently found in dairy products and dairy environment. In this study, the hydrophobicity and surface electrical charge of spores from fourteen (14) Bacillus cereus strains isolated from a dairy plant located in north-western Algeria (were studied using microbial adhesion to hydrocarbon (MATH) method, and zeta potential measurements, respectively. Spores of eleven (11)...
January 2015
Prevalence and distribution of methicillin resistant Staphylococcus aureus (MRSA) among laboratory science students and laboratory staff from a single hospital in North Saudi Arabia
Staphylococcus aureus causes morbidity and mortality in both community and hospital settings. Methicillin resistant S. aureus (MRSA) is being increasingly reported globally. This study aimed at finding out the prevalence and the distribution of the community-associated MRSA (CA-MRSA) or healthcare-associated MRSA (HA-MRSA) among 150 subjects. Of the 150 subjects, 125 were students of laboratory science and 25 were...
January 2015
Characterization and identification of lactic acid bacteria isolated from traditional cheese (Klila) prepared from cow's milk
Various types of fermented dairy products exist worldwide. Their nature depends on the type of milk used, pretreatment, fermentation conditions and subsequent treatment. The fermentation of milk primarily involves lactic acid bacteria (LAB). Among these, the Klila is a hard variety cheese made by using the traditional procedures in the home, without using a starter culture. The different samples of traditional cheese...
January 2015
β-Lactam - β-lactamase inhibitor combinations as the choice therapy for multidrug resistant Acinetobacter
Acinetobacter, an important nosocomial pathogen, is capable of causing infectious outbreaks in critically ill patients which results into high morbidity and mortality worldwide. It is rated among top seven pathogens that disturb the health care delivery system. The situation has become complicated due to the organism’s capability to acquire diverse resistance mechanisms. This has resulted in the emergence of...
January 2015
Identification of volatile compounds, antimicrobial properties and antioxidant activity from leaves, cones and stems of Cupressus sempervirens from Algeria
Cupressus sempervirens L. (Cupressaceae) leaves, cones and young branches have been used in traditional medicine and aromatherapy. The composition of the isolates obtained by hydrodistillation from the aerial parts (plant material collected at Bainam forest, in Northwest of Alger, Algiers), were analyzed by GC and GC-MS. The leaves isolate (yield 0.22% w/w), was mainly composed of monoterpene hydrocarbons (60.8%),...
January 2015
Obtaining DNA from Staphylococcus aureus: A study on DNA extraction methods for food matrices without bacterial isolation
Polymerase chain reaction (PCR) is a tool widely used. However, for the effective use of PCR, DNA quality is necessary. Thereby, the objective of this study was to evaluate DNA extraction methods of Staphylococcus aureus, for direct application in food. Five methods were tested using reference and food strains. The methods show different characteristics with steps of thermal lysis, enzymatic lysis, detergents and...
January 2015
Induction of defense-related proteins and growth promotion in tomato by mixture of Trichoderma harzianum OTPB3 and Bacillus subtilis OTPB1 and Pseudomonas putida OPf1 against Phytophthora infestans
Late blight incited by Phytophthora infestans is a destructive disease of tomato worldwide. The plant growth-promoting antagonists, which elicit induced systemic resistance (ISR) and enhance plant growth, are being used as safe alternatives to synthetic fungicides for the management of plant diseases. In this study, a combination of Trichoderma harzianum OTPB3 and Bacillus subtilis OTPB1 and Pseudomonas putida OPf1...
January 2015
Microbiological assessment of traditional smoked silver catfish (Chrysichthys nigrodigitatus)
Smoked fish is relished food item in many dishes in Nigeria. Traditional smoked silver catfish (Chrysichthys nigrodigitatus) that floods the smoked fish market of the Lagos State of Nigeria are not microbiologically shelf-stable; hence, the need for a study on their microbiological quality and safety. ‘Fresh silver catfish’ (100 samples) and smoked silver catfish (100 samples) were collected from 20...
January 2015
Inducing salinity tolerance in chickpea (Cicer arietinum L.) by inoculation of 1-aminocyclopropane-1-carboxylic acid deaminase-containing Mesorhizobium strains
Salinity stress severely affects growth, nodulation and yield of chickpea (Cicer arietinum L.). However, inoculation with Mesorhizobium strains containing 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase improves the plant growth by reducing the level of ethylene induced by salt stress. Fifty (50) Mesorhizobium isolates were obtained from nodules of chickpea plants on yeast extract mannitol agar (YEMA) medium....
January 2015
Effects of gelatin coating glass coverslips on fungal attachment and their morphological demonstrations
Gelatin obtained from collagen, an extracellular matrix (ECM) protein, plays a crucial role in facilitating cell attachment. This study aimed to determine the effects of gelatin on fungal attachment and morphology. Samples of Aspergillus niger were grown on gelatin-coated coverslips and non-gelatin-coated coverslips, and prepared for examination under both light and scanning electron microscopes. The results show...
January 2015
Antimicrobial activity of extracts from an endemic Salvia cilicica Boiss. and Kotschy
The antimicrobial activities of the different aerial and root extracts of Salvia cilicica were determined aiming to evaluate whether, it can be used in phytotherapy as an antimicrobial agent. In this study, the antimicrobial activity of roots and aerial parts of S. cilicica extracts was evaluated using micro dilution and disc diffusion methods against Gram positive and Gram negative reference standard microorganisms and...
January 2015
Detection of Leptospira interrogans in plasma and urine samples by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)
Acute phase of leptospirosis has high mortality. A diagnosis can be designed with high sensitivity by testing blood and urine samples by polymerase chain reaction (PCR) for early diagnosis of this phase of disease. The aim of this research was to detect Leptospira interrogans by PCR technique in plasma and urine samples which were taken from symptomatic patients. Clinical isolates were obtained in hospitals of north...
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