African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5189

Table of Content: 18 July; 4(14)

July 2010

Screening and fermentation optimization of microbial lipid-producing molds from forest soils

  Sixty-one isolates including molds, yeasts and bacteria from the forest soils collected from Hunan Province, China were tested for their potentiality to accumulate microbial lipid as alternative sources for biodiesel production. The results showed that sixteen mold isolates were potential oleaginous microorganisms, among which, strain SCIM 3.009 was the best lipid producers, which may accumulate up to 50.4%...

Author(s): Gao-Qiang Liu, Qin-Lu Lin, Xian-Chun Jin, Xiao-Ling Wangand Yan Zhao

July 2010

Cloning and expression of C-terminal of Clostridium perfringens type A enterotoxin and its biological activity

Pathogenic clostridia produce exocellular toxins that resemble lipoteichoic acid and are described as super antigens. These toxins stimulate T-cell receptor-carrying lymphocytes in peripheral blood and have been used to study immunodeficiency diseases and cancers. The CPE C-terminal region from one of the local type A strain was cloned in the pET32a vector its expression induced with IPTG. The expressed protein was...

Author(s): Atieyeh Taherian Fard, Fariha Hasan, Mojgan Bandehpour, Nariman Mosaffa, Fatemeh Mashhadi Abbas, Abdul Hameed, Aamer Ali Shah and Bahram Kazemi

July 2010

Bacteria associated with bovine dermatophilosis in Zaria, Nigeria

  A study was carried out to determine the type of bacteria associated with bovine dermatophilosis in Zaria, Nigeria. Skin samples obtained from two hundred and eleven cattle with skin lesions suspected to be dermatophilosis were processed for bacteriology. One hundred and sixty-seven (79.1%) samples were positive forDermatophilus congolensis, while 44 (20.9%) were negative. Both D....

Author(s): J. S. Dalis, H. M. Kazeem, A. A. Makinde and M. Y. Fatihu

July 2010

Comparison of the effect of particulate materials and some osmoregulators on lactic fermentation of new local white cassava variety “Bianbasse” using both spontaneous and starter cultures

  The effects of particulate materials and osmoregulatators on lactic fermentation of cassava were determined on total dissolved loads of all the samples; the total reducing sugars of all samples, the microbial loads in all the samples, the percentage crude protein contents, crude fibres, crude fat/ether, ash, phytic acid and tannin. Sample A1 inoculated with varied concentrations of particulate materials...

Author(s): L. A. Adetunde and A. A. Onilude

July 2010

Prevalence of Mycobacterium tuberculosis and human immunodeficiency virus (HIV) infections in Umuahia, Abia State, Nigeria

The prevalence of Mycobacterium tuberculosis (TB) and human immunodeficiency virus (HIV) infections was investigated among individuals from age 16 years and above attending out patient clinic in federal medical centre (FMC) and other hospitals in Umuahia. A total of two hundred and fifty individuals were examined. The examinations of the samples from the patients were done according to bacteriological and...

Author(s): Ejikeme Nwachukwu and Godwin Aguziendu Peter

July 2010

Plant growth promoting activities of fluorescent pseudomonads associated with some crop plants

  Fluorescent pseudomonads (FP), a major component of rhizobacteria, promote the plant growth through their multifarious activities. In the present investigations, 10 strains of fluorescent pseudomonads isolated from the rhizosphere soils of bajra (Pennisetum glaucum), jowar (Sorghum vulgare), rice (Oryza sativa) and maize (Zea mays) were screened for their plant growth promoting activity based on their ability...

Author(s): A. Suresh, P. Pallavi, P. Srinivas, V. Praveen Kumar, S. Jeevan Chandra and S. Ram Reddy

July 2010

Infection pathways of soft rot pathogens on Amorphophallus konjac

  With the rapid expansion of cultivated land, bacterial soft rot of konjac is more and more serious. The main pathogen is Erwinia carotovora subsp. carotovora. Some researchers think the natural openings and wounds are the main infection pathways on konjac. In this study, the infection pathways are investigated. From macro-observation, the soft-rot pathogens could not directly invade the intact...

Author(s): Jinping Wu, Ying Diao, Yucheng Gu and Zhongli Hu

July 2010

Epidemiological and immunological findings on human hydatidosis

  30 patients infected with hydatidosis from different hospitals in Baghdad were included in this study. All these patients were diagnosed by imaging techniques and surgically proven, immunological study were performed on blood samples. The study showed that the incidence of hydatidosis in females was higher than that of the males. The highest age distribution of hydatidosis patients was between 12 and 40...

Author(s): Ihsan E. Al-Saimary, Maani N. Al-Shemari and Mohammed M. A. Al-Fayadh

July 2010

Use of Scopulariopsis acremonium for the production of cellulase and xylanase through submerged fermentation

  Production of cellulase and xylanase by Scopulariopsis acremonium through submerged fermentation was studied using shake flask cultivation media. The effect of process parameters such as effect of temperature, pH and inoculum size was investigated. The enzymes were partially purified using isopropanol and used for enzyme assays. The results indicated that the maximum cellulase and xylanase having an...

Author(s): Loveleen Kaur Sarao, Maninder Arora and V. K. Sehgal

July 2010

Use of RT-PCR in diagnosis of infectious hematopoietic necrosis in rainbow trout hatcheries, Iran

  Distribution of Infectious Hematopoietic Necrosis (IHN) was studied in rainbow trout fish farms in Chaharmahal and Bakhtyari Province which is a major trout culture region in Iran. Overall 150 suspected rainbow trout fry with less than 3 g body weight from 30 fish farms in Chaharmahal and Bakhtyari Province, Iran, were studied and fishes of 10 hatcheries (33.3% of all studied farms) found to be infected with...

Author(s): Mehdi Raissy, Hasan Momtaz, Mahsa Ansari and Manouchehr Moumenia

July 2010

Protease and amylase enzymes for biofilm removal and degradation of extracellular polymeric substances (EPS) produced by Pseudomonas fluorescens bacteria

  Removal of biofilms is difficult. In industrial settings, both the inactivation and removal of biofilms are of huge concern. If only disinfection without the removal of attached biofilms occurs, the inactivated biofilm cells may provide an ideal environment for further adhesion and growth, resulting in a complex matrix. Microbial resistance to biocides and their negative environmental impact are the main...

Author(s): I. Phyllis Molobela, T. Eugene Cloete and Mervyn Beukes

July 2010

Antibacterial effect of chlorine dioxide and hyaluronate on dental biofilm

  The objective of this study is to investigate antimicrobial action of chlorine dioxide (ClO2) gel and hyaluronate gel (Gengigel®) on dental biofilm. Pooled supra and subgingival dental biofilm were obtained from healthy individuals and incubated aerobically and anaerobically. Plaque bacteria investigated...

Author(s): F. Al-bayaty, T. Taiyeb-ali, M. A. Abdulla and F. Hashim

July 2010

The prevalence of group A rotavirus infection and some risk factors in pediatric diarrhea in Zaria, North central Nigeria

  Group A rotavirus cause infectious, severe and dehydrating diarrhea which have great impact on childhood morbidity and mortality worldwide. This study was designed to investigate the disease burden and risk factors of rotavirus infection among children 1 – 5 years in Zaria, Nigeria. Stool samples were collected from 666 diarrheic children and 170 matched non-diarrheic controls and screened for rotavirus...

Author(s): Pennap Grace and Umoh Jerald

July 2010

Isolation and identification of polystyrene biodegrading bacteria from soil

  With the increased production of municipal solid waste by the disposal of plastic materials, there is a need to develop new biodegradable materials and biodegrade existing plastic materials in daily use. Polystyrene and expanded polystyrene are commodity plastics that are extensively used in packaging and other applications. Six bacterial isolates were isolated from soil buried expanded polystyrene films...

Author(s): Naima Atiq, Safia Ahmed, M. Ishtiaq Ali, Saadia Andleeb, Bashir Ahmad and Geoffery Robson

July 2010

Investigation of antibacterial activity of Streptomycetes isolates from soil samples, West of Iran

  In this research our goal is the determination of Streptomyces species with antimicrobial activity from some regions of northwest of Iran. The future studies will be performed to investigate the type of antimicrobial agents. In order to achieve to this aim, following the collection of the soil samples, the samples were diluted and cultured in SCA medium. The Actinomycetes were...

Author(s): Alireza Dehnad, Lalle Parsa, Rouhollah Bakhshi, Samad abdi soofiani and Ahad Mokhtarzadeh

July 2010

Isolation, identification and characterization of multiple enzyme producing actinobacteria from sediment samples of Kodiyakarai coast, the Bay of Bengal

  A total of twenty nine strains were isolated from the Kodiyakarai coastal sediments (Bay of Bengal) and ten of them exhibited multiple enzyme activity. Of these, the best (GK-22) was selected based on zone formation (amylase, cellulase and protease) and its growth conditions were standardized for better production of multiple enzymes. Present study on production of multiple enzymes (amylase, cellulase and...

Author(s): P. Manivasagan, S. Gnanam, K. Sivakumar, T. Thangaradjou, S. Vijayalakshmi and T. Balasubramanian

July 2010

Studies on major respiratory diseases of Camel (Camelus dromedarius) in Northeastern Ethiopia

  A comparative serological test was carried out to identify the primary causative agent involved in the camel respiratory disease outbreak that occurred in Ethiopia. The samples were collected during and post outbreak time and analyzed forParainfulenza-3 and pasteurellosis. According to the result, Parainfulenza-3 was found as a primary causative agent as 70.5% in outbreak and 6.8% in surveyed...

Author(s): Fekadu Kebede and Esayas Gelaye

July 2010

Distribution of wild poliovirus genotypes in India

  In order to study the genetic relationships and epidemiological links between strains circulating in the Vellore region of India, 70 wild type poliovirus strains isolated from paralytic poliomyelitis cases were sequenced at the VP1-2A junction region of the viral genome. This showed that three genotypes of types 1 and 2 and four genotypes of type 3 poliovirus were circulating in India at least since 1985....

Author(s): George Dahourou, Benjamin Samuel, and Radu Crainica

July 2010

Association among the Rv2629 gene, rpoB gene, RFP resistance and Beijing genotype in Mycobacterium tuberculosis clinical isolates

  Rifampin (RFP) is a major first-line anti-tuberculosis drug. The molecular assay currently used is the detection of rpoB gene mutations in Mycobacteriumtuberculosis. Recently, the Rv2629 191C allele was found to have a correlation with RFP resistance, and might become a valuable marker for the detection of RFP resistance or the Beijing genotype. We studied the association among the Rv2629gene, rpoB gene,...

Author(s): Junxian Zhang, Xueqiong Wu, Yourong Yang, Yan Zhuang, Bo Wang, Jianqin Liang, Yan Liang, Hongmin Li and Chuihuan Li

July 2010

Frequency and anti-biogram pattern of coagulase negative Staphylococcus in clinical specimens of Shahid Mohammadi Hospital in patients, Bandar-Abbas, Iran

  Coagulase negative staphylococci (CoNS), commonly known as a part of normal-biota of human body, have become predominant pathogens over the last decades. Due to the vast use of antibiotics for therapy or prophylaxis, those CoNS strains acquired in the hospitals have become resistant to various antimicrobial agents. This descriptive cross-sectional study was carried out from December 2007 to June 2008 on 5063...

Author(s): Sedigheh Javadpour,  Ehsan Karimi and Afsaneh Karmostaji