February 2014
Morphological and molecular characterization of pathogenic isolates of Fusarium spp. obtained from gladiolus corms and their sensitivity to Jatropha curcas L. oil
The State of Morelos is the third biggest gladiolus producer in Mexico. However, this ornamental is affected by the disease named corm rot or fusarium yellows, characterized by leaf yellowing, epinasty and wilting, and caused by fungi of the genus Fusarium. The first objective was to corroborate the pathogenicity of the 45 isolates obtained. The second objective was to identify and characterize morphologically and...
February 2014
Screening phosphate solubilizing actinobacteria isolated from the rhizosphere of wild plants from the Eastern Cordillera of the Colombian Andes
Colombia is a tropical country with high diversity and an agricultural economy, yet their soils are characterized by low pHs and poor phosphorus concentrations. Soil supplementation with chemical fertilizers containing soluble phosphorus is a costly and contaminating practice and for this reason, the aim of this study was to isolate actinobacteria that are able to release soluble phosphate from wild plants of the...
February 2014
Establishment of EvaGreen qPCR for detecting bovine rotavirus based on VP7 gene
The aim of the present study was to establish a method of EvaGreen real time fluorescence quantitative polymerase chain reaction (qPCR) for detecting quickly bovine rotavirus (BRV) in fecal samples from calves with diarrhea. The specific primers were designed and synthesized according to BRV VP7 gene in Genbank. VP7 gene was cloned into the pMD18-T vector. The bovine viral diarrhea virus, bovine coronavirus, porcine...
February 2014
Nutritional requirements for the production of antimicrobial metabolites from Streptomyces
The objective of this study was to optimize the nutritional and cultural conditions of Streptomyces strain ERI-1, ERI-3 and ERI-26 for the production of antimicrobial metabolites under shake-flask conditions. Effect of eight fermentation medium, different temperature, pH, incubation time, different carbon and nitrogen sources and different concentration of sodium chloride on production of antimicrobial metabolites were...
February 2014
Incidence of Aspergillus contamination of groundnut (Arachis hypogaea L.) in Eastern Ethiopia
The production of groundnut is constrained by several factors, among which is Aspergillus spp. In addition to causing quantitative losses, Aspergillus spp. produce highly toxic and carcinogenic chemical substances known as aflatoxins. This study was conducted with the objectives to (i) identify Aspergillus species associated with groundnuts, (ii) determine the frequency of seed contamination, and (iii) survey...
February 2014
Microencapsulation, survival and adherence studies of indigenous probiotics
The aim of the study was to evaluate the adherence potential of indigenous probiotic bacteria and to improve the gastrointestinal survival of these cultures by adopting the double microencapsulation technique. The mean with standard deviation of triplicate experiments for the cell surface hydrophobicity, aggregation, and Cell adhesion evaluation of indigenous probiotics revealed that there was no significant difference...
February 2014
Screening novel diagnostic marker of Mycobacterium tuberculosis
The aim of present study was to screen and evaluate the serodiagnostic value of the purified fusion antigens of Mycobacterium tuberculosis (Mtb) by enzyme-linked immunosorbent assay (ELISA). A group of vector system which was constructed by cloning Rv1908c, Rv0733, Rv0899, Rv1411c and Rv3914 gene of Mtb into the prokaryotic expression plasmid pET-32b was transformed into E. coli for induction and expression fusion...
February 2014
An outbreak of ringworm caused by Trichophyton verrucosum in a group of calves in Vom, Nigeria
An outbreak of ringworm in young calves is reported from Vom in Nigeria. Twelve out of fourteen calves were observed to have skin lesions consistent with dermatophytosis. Lesions were seen mostly around the eyes and neck. Skin scrapings were collected from the affected areas and processed for mycology. Trichophyton verrucosum was isolated from all the affected calves. This study has shown that T. verrucosum can be a...
February 2014
Characteristics of nodule bacteria from Mimosa spp grown in soils of the Brazilian semiarid region
The Brazilian Northeastern dry forest (Caatinga) is one of the diversification centers of Mimosa species. We determined the characteristics of native rhizobia isolates from nodules of Mimosa tenuiflora and Mimosa paraibana grown in pots with soils collected under Caatinga vegetation and compared the restriction ribosomal DNA profiles of the isolates with those of 16 reference strains. All plants formed abundant...
February 2014
Microbiological assessment of dentists’ hands in clinical performance
This study verified the presence of opportunist pathogenic bacteria in the hands of Surgeons Dentists. Biological materials were collected from the hands of 41 professionals, randomly selected. The professional washed his/her hands for around 30 s in a sterile plastic bag, filled with 250 mL of physiologic serum, in three distinct times: Timing 1 (T1), hands without wearing gloves, before attending to the patient;...
February 2014
Evaluation of marine macro alga, Ulva fasciata against bio-luminescent causing Vibrio harveyi during Penaeus monodon larviculture
Vibrio harveyi is one of the major disease causing bacterium in shrimp larviculture and grow-out practices. V. harveyi produces many virulence cum pathogenic factors. Application of antibiotics against luminescence causes development of antibiotic resistance among V. harveyi. Therefore, it is obligatory to develop bio-inhibitory agents as substitute in lieu of antibiotics. Under this study, Ulva fasciata was collected...
February 2014
Characterization and determination of antibiotic susceptibility pattern of bacteria isolated from some fomites in a teaching hospital in northern Nigeria
Fomites are inanimate objects that can serve as vehicles for pathogens transfer. This study was conducted to determine the antibiogram of bacteria isolated from fomites in a teaching hospital in Nigeria. Exactly 35 samples were used for the study. Twenty three (65.7%) isolates were obtained; the ratio of Gram positive to Gram negative organisms was 12 to 11. The bacteria isolated were Staphylococcus aureus...
February 2014
Prevalence of different enterococcal species isolated from blood and their susceptibility to antimicrobial drugs in Vojvodina, Serbia, 2011-2013
Enterococci are one of the leading causes of nosocomial infections worldwide. Enterococcus faecalis and Enterococcus faecium are the most commonly isolated. The aim of this study was to determine prevalence of these species isolated from blood samples of hospitalized patients and their susceptibility to antibiotics particularly to vancomycin and high concentrations of aminoglycosides. A total of 89 enterococcal strains...
February 2014
Prevalence of anti-Anaplasma phagocytophilum antibodies among dogs from Monterrey, Mexico
Infection with Anaplasma phagocytophilum is of importance in dogs. It is called canine anaplasmosmosis and causes persistent clinical signs for long periods of time. Its main vector is a tick of the genus Ixodes. Diagnosis of anaplasmosis can be achieved either by serological or molecular methods. Presence of the disease has been reported in the U.S.A., Europe and Asia, but little is known about its prevalence in...
February 2014
Novel simple diagnostic methods compared to advanced ones for the diganosis of Chlamydia trachomatis, Ureaplasma urealyticum and Mycoplasmas hominis in patients with complicated urinary tract infections
Chlamydia trachomatis is responsible for the most common sexually transmitted bacterial infection worldwide. Infections with Ureaplasma urealyticum and genital Mycoplasma hominis have been recognized for about a decade as common sexually transmitted infections (STIs) in developed countries. The aim of this study was to assess the diagnostic value of novel simple diagnostic kits used in the detection of C. trachomatis,...
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